In the recent decade,GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties,whereas similar research based on rGO was reported rarely.Herein,a nov...In the recent decade,GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties,whereas similar research based on rGO was reported rarely.Herein,a novel multi-pyrene functionalized G-rich DNA probe based on the screened rGO showed much higher fluorescence quenching efficiency and excimer emission than that of universal GO.Different from the universal thrombin detection of the G4-forming aptamer-TBA(GGTTGGTGTGGTTGG),the original telomeric sequence is used in this study.The excimer emission“ON-OFF”switch amplified the response of thrombin detection is as low as 50 units.Furthermore,for four pyrene moieties that are sited in a crowded steric circumstance,the melting temperature(T_(m))values and molecular dynamics simulations showed a positive effect on duplex G-quadruplex or _(G)DNA·_(c)DNA stability,without disturbing its helix structure.展开更多
基金supported by the Science and Technology Innovation Commission of Shenzhen,China(Nos.KQJSCX20180328095517269 and JCYJ20210324095607021)。
文摘In the recent decade,GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties,whereas similar research based on rGO was reported rarely.Herein,a novel multi-pyrene functionalized G-rich DNA probe based on the screened rGO showed much higher fluorescence quenching efficiency and excimer emission than that of universal GO.Different from the universal thrombin detection of the G4-forming aptamer-TBA(GGTTGGTGTGGTTGG),the original telomeric sequence is used in this study.The excimer emission“ON-OFF”switch amplified the response of thrombin detection is as low as 50 units.Furthermore,for four pyrene moieties that are sited in a crowded steric circumstance,the melting temperature(T_(m))values and molecular dynamics simulations showed a positive effect on duplex G-quadruplex or _(G)DNA·_(c)DNA stability,without disturbing its helix structure.
