This study was conducted to valorize four sources of fiber (Gliricidia sepium, Leucaena leucocephala, Moringa oleifera and palm nut fibers) in animal production. The experiments were carried out on 128 rabbits divided...This study was conducted to valorize four sources of fiber (Gliricidia sepium, Leucaena leucocephala, Moringa oleifera and palm nut fibers) in animal production. The experiments were carried out on 128 rabbits divided into 4 batches of 32 animals, reared for 8 weeks under the same conditions. Each batch was subdivided into two subgroups, one fed with a complete diet (a diet containing one of the fiber sources and served all day) and the other with the same diet separated from the fiber source (served at 9 a.m. and supplemented with the fiber source at 4 p.m.). Eight (8) experimental rations were, respectively, tested on the subgroups: complete feed Gliricidia sepium (CFG);supplemented feed Gliricidia sepium (SFG);complete feed Leucaena leucocephala (CFL);supplemented feed Leucaena leucocephala (SFL);complete feed Moringa oleifera (CFM);supplemented feed Moringa oleifera (SFM);complete feed palm nut fiber (CFF);supplemented feed palm nut fiber (SFF). In each subgroup, 4 rabbits were slaughtered at 15 weeks of age for a total of 32 rabbits. Physico-chemical parameters were evaluated on the feed and the meat. Data were analyzed using SAS 2013 software. Fiber content was similar (p > 0.05) for complete feeds. Fat content was high (p < 0.001) for the palm nuts fiber (27.34%) and the CFF feed (11.36%). Feeding rabbits with G. sepium leaves or palm nut fiber continuously increased the fat content of the meat in contrast to sequential feeding. Meat quality was also better when the fiber source was used in the feed of the rabbits in the evening.展开更多
Fiber length is one of the most important quality parameters of cotton fibers.Transcriptomic analyses of developing cotton fibers have identified genes preferentially expressing in fiber elongation stage,but few have ...Fiber length is one of the most important quality parameters of cotton fibers.Transcriptomic analyses of developing cotton fibers have identified genes preferentially expressing in fiber elongation stage,but few have been functionally characterized.Here,on the basis of confirmation of the preferential expression profile of GhAlaRP(Gh_A09G1166 and Gh_D09G1172),an alanine rich protein gene,in the rapid elongating fibers,we investigated the role of GhAlaRP in fiber development by generating transgenic cottons with an increased or decreased expression level of GhAlaRP.Our results showed that the fiber length was consistently significantly shorter in both the GhAlaRP-RNAi lines and the alarp mutant generated by genome editing than in the control YZ-1.GhAlaRP was localized on plasma membrane,nucleus and endoplasmic reticulum.The yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that GhAlaRP co-expresses and interacts with GhAnnexin(Gh_D11G2184)and GhEXPA(Gh_A10G2323)that are involved in fiber elongation.Down-regulation of GhAlaRP co-suppressed the expression levels of GhAnnexin and GhEXPA.These results suggest a role of GhAlaRP in regulation of cotton fiber elongation,which could be achieved by regulating the functions of GhAnnexin and GhEXPA.展开更多
The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of ...The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of the coagulation bath and coagulating time on the absorptive ability of alginate fiber were discussed. The results show that the absorptive ability to different substances varies in such order： physiological saline 〉 blood 〉 tap water 〉distilled water. The absorptive ability of the alginate fiber increases with the increase of M/G value and the concentration of sodium-alginate. Gradually increasing the concntration of coagulation bath, it increases until the concentration is up to 5%. However, it reduces with lasting coagulating tine.展开更多
Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However...Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.展开更多
文摘This study was conducted to valorize four sources of fiber (Gliricidia sepium, Leucaena leucocephala, Moringa oleifera and palm nut fibers) in animal production. The experiments were carried out on 128 rabbits divided into 4 batches of 32 animals, reared for 8 weeks under the same conditions. Each batch was subdivided into two subgroups, one fed with a complete diet (a diet containing one of the fiber sources and served all day) and the other with the same diet separated from the fiber source (served at 9 a.m. and supplemented with the fiber source at 4 p.m.). Eight (8) experimental rations were, respectively, tested on the subgroups: complete feed Gliricidia sepium (CFG);supplemented feed Gliricidia sepium (SFG);complete feed Leucaena leucocephala (CFL);supplemented feed Leucaena leucocephala (SFL);complete feed Moringa oleifera (CFM);supplemented feed Moringa oleifera (SFM);complete feed palm nut fiber (CFF);supplemented feed palm nut fiber (SFF). In each subgroup, 4 rabbits were slaughtered at 15 weeks of age for a total of 32 rabbits. Physico-chemical parameters were evaluated on the feed and the meat. Data were analyzed using SAS 2013 software. Fiber content was similar (p > 0.05) for complete feeds. Fat content was high (p < 0.001) for the palm nuts fiber (27.34%) and the CFF feed (11.36%). Feeding rabbits with G. sepium leaves or palm nut fiber continuously increased the fat content of the meat in contrast to sequential feeding. Meat quality was also better when the fiber source was used in the feed of the rabbits in the evening.
基金This work was supported by the National Key Research and Development Program of China(2016YFD0101900,2017YFD0100200)the Genetically Modified Organisms Breeding Major Project of China(2016ZX08005-005).
文摘Fiber length is one of the most important quality parameters of cotton fibers.Transcriptomic analyses of developing cotton fibers have identified genes preferentially expressing in fiber elongation stage,but few have been functionally characterized.Here,on the basis of confirmation of the preferential expression profile of GhAlaRP(Gh_A09G1166 and Gh_D09G1172),an alanine rich protein gene,in the rapid elongating fibers,we investigated the role of GhAlaRP in fiber development by generating transgenic cottons with an increased or decreased expression level of GhAlaRP.Our results showed that the fiber length was consistently significantly shorter in both the GhAlaRP-RNAi lines and the alarp mutant generated by genome editing than in the control YZ-1.GhAlaRP was localized on plasma membrane,nucleus and endoplasmic reticulum.The yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that GhAlaRP co-expresses and interacts with GhAnnexin(Gh_D11G2184)and GhEXPA(Gh_A10G2323)that are involved in fiber elongation.Down-regulation of GhAlaRP co-suppressed the expression levels of GhAnnexin and GhEXPA.These results suggest a role of GhAlaRP in regulation of cotton fiber elongation,which could be achieved by regulating the functions of GhAnnexin and GhEXPA.
基金The Research Fund for the Doctoral Program of Higher Education(No. 20061065002)The Research on Science and Technology Item of Shandong Province (No. 2006GG3205005)
文摘The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of the coagulation bath and coagulating time on the absorptive ability of alginate fiber were discussed. The results show that the absorptive ability to different substances varies in such order： physiological saline 〉 blood 〉 tap water 〉distilled water. The absorptive ability of the alginate fiber increases with the increase of M/G value and the concentration of sodium-alginate. Gradually increasing the concntration of coagulation bath, it increases until the concentration is up to 5%. However, it reduces with lasting coagulating tine.
基金the State Key Laboratory of Cotton Biology Open Fund(CB2019A03,2019A09 and CB2020A12)the National Natural Science Foundation of China(31872175)+2 种基金the Fundamental Research Funds for the Central Universities(GK202002005 and GK202001004)Natural Science Basic Research Plan in Shaanxi Province of China(2018JZ3006 and 2019JQ-062),Shaanxi Youth Entrusted Talents Program(20190205),Shaanxi Postdoctoral Project(2018BSHYDZZ76)Young Elite Scientists Sponsorship Program by CAST(2019-2021QNRC001).
文摘Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.
