On the basis of analyzing both domestic and foreign evaluation systems for the e-government and also taking into account of the current situation of government portals in urban centers,the paper is aimed to construct ...On the basis of analyzing both domestic and foreign evaluation systems for the e-government and also taking into account of the current situation of government portals in urban centers,the paper is aimed to construct an evaluation system primarily for the measurement of the service capability of business-oriented G2B portals of local governments in general and those G2B portals of the new development zones in particular.This system is composed of 6 first tier indicators,18 second tier indicators and 90 third tier indicators.And the weight of each indicator is determined by Delphi Method and Analytic Hierarchy Process(AHP) Method.Subsequently,we used this system to evaluate the government portals of Tianjin Binhai New Area and Shanghai Pudong New Area and proved to have the desired practicability.展开更多
BACKGROUND Phospholipase A2(PLA2)enzymes are pivotal in various biological processes,such as lipid mediator production,membrane remodeling,bioenergetics,and maintaining the body surface barrier.Notably,these enzymes p...BACKGROUND Phospholipase A2(PLA2)enzymes are pivotal in various biological processes,such as lipid mediator production,membrane remodeling,bioenergetics,and maintaining the body surface barrier.Notably,these enzymes play a significant role in the development of diverse tumors.AIM To systematically and comprehensively explore the expression of the PLA2 family genes and their potential implications in cholangiocarcinoma(CCA).METHODS We conducted an analysis of five CCA datasets from The Cancer Genome Atlas and the Gene Expression Omnibus.The study identified differentially expressed genes between tumor tissues and adjacent normal tissues,with a focus on PLA2G2A and PLA2G12B.Gene Set Enrichment Analysis was utilized to pinpoint associated pathways.Moreover,relevant hub genes and microRNAs for PLA2G2A and PLA2G12B were predicted,and their correlation with the prognosis of CCA was evaluated.RESULTS PLA2G2A and PLA2G12B were discerned as differentially expressed in CCA,manifesting significant variations in expression levels in urine and serum between CCA patients and healthy individuals.Elevated expression of PLA2G2A was correlated with poorer overall survival in CCA patients.Additionally,the study delineated pathways and miRNAs associated with these genes.CONCLUSION Our findings suggest that PLA2G2A and PLA2G12B may serve as novel potential diagnostic and prognostic markers for CCA.The increased levels of these genes in biological fluids could be employed as non-invasive markers for CCA,and their expression levels are indicative of prognosis,underscoring their potential utility in clinical settings.展开更多
基金supported by the Korea Foundation for Advanced Studies and Asia Research Center of Nankai University(Grant No.AS0719)
文摘On the basis of analyzing both domestic and foreign evaluation systems for the e-government and also taking into account of the current situation of government portals in urban centers,the paper is aimed to construct an evaluation system primarily for the measurement of the service capability of business-oriented G2B portals of local governments in general and those G2B portals of the new development zones in particular.This system is composed of 6 first tier indicators,18 second tier indicators and 90 third tier indicators.And the weight of each indicator is determined by Delphi Method and Analytic Hierarchy Process(AHP) Method.Subsequently,we used this system to evaluate the government portals of Tianjin Binhai New Area and Shanghai Pudong New Area and proved to have the desired practicability.
基金Supported by the Key Specialty Construction Project of Shanghai Pudong New Area Health Commission,No.PWZzk2022-17Shanghai East Hospital Clinical Research Project,No.DFLC2022019and the Featured Clinical Discipline Project of Shanghai Pudong District,No.PWYts2021-06.
文摘BACKGROUND Phospholipase A2(PLA2)enzymes are pivotal in various biological processes,such as lipid mediator production,membrane remodeling,bioenergetics,and maintaining the body surface barrier.Notably,these enzymes play a significant role in the development of diverse tumors.AIM To systematically and comprehensively explore the expression of the PLA2 family genes and their potential implications in cholangiocarcinoma(CCA).METHODS We conducted an analysis of five CCA datasets from The Cancer Genome Atlas and the Gene Expression Omnibus.The study identified differentially expressed genes between tumor tissues and adjacent normal tissues,with a focus on PLA2G2A and PLA2G12B.Gene Set Enrichment Analysis was utilized to pinpoint associated pathways.Moreover,relevant hub genes and microRNAs for PLA2G2A and PLA2G12B were predicted,and their correlation with the prognosis of CCA was evaluated.RESULTS PLA2G2A and PLA2G12B were discerned as differentially expressed in CCA,manifesting significant variations in expression levels in urine and serum between CCA patients and healthy individuals.Elevated expression of PLA2G2A was correlated with poorer overall survival in CCA patients.Additionally,the study delineated pathways and miRNAs associated with these genes.CONCLUSION Our findings suggest that PLA2G2A and PLA2G12B may serve as novel potential diagnostic and prognostic markers for CCA.The increased levels of these genes in biological fluids could be employed as non-invasive markers for CCA,and their expression levels are indicative of prognosis,underscoring their potential utility in clinical settings.
