Latent membrane protein 1 （LMP1）, an important protein encoded by Epstein Barr virus （EBV）, has been implied to link with the pathogenesis of nasopharyngeal carcinoma （NPC）. Its dual effects of increasing cell p...Latent membrane protein 1 （LMP1）, an important protein encoded by Epstein Barr virus （EBV）, has been implied to link with the pathogenesis of nasopharyngeal carcinoma （NPC）. Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMPI. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC.展开更多
目的：探讨G-蛋白信号转导调节子4（regulator of G-protein signaling-4，RGS4）基因与精神分裂症及临床症状的遗传关联。方法：应用病例对照关联研究设计，采用聚合酶链式反应-限制性片断长度多态性（PCR-RFLP）和DNA测序方法，分析38...目的：探讨G-蛋白信号转导调节子4（regulator of G-protein signaling-4，RGS4）基因与精神分裂症及临床症状的遗传关联。方法：应用病例对照关联研究设计，采用聚合酶链式反应-限制性片断长度多态性（PCR-RFLP）和DNA测序方法，分析386例精神分裂症患者和390例正常对照者中RGS4基因4个单核苷酸多态性（SNP）位点与精神分裂症的关联。并采用阳性和阴性症状量表（PANSS）评估患者的临床症状，进一步分析PANSS因子分与RGS4多态性的关联。结果：RGS4基因的两个多态性位点rs12753561（T〉G，χ^2=8．970，P=0．002）和rs10759（C〉A，χ^2=13．773，F=0．002，P=0．002）与精神分裂症关联，由上述4个SNPs组成的多个单体型如AAGA（χ^2=11．120，P=0．0008，OR=0．52，95％CI=0．36—0．77）和GGGC（χ^2=10．096，P=0．001，OR=1．43，95％CI=1．15—1．79）均与精神分裂症关联。PANSS量表的阴性症状因子分与rs12753561（t=2．216，P=0．029）和rs10759（t=2．543，P=0．012）关联。结论：RGS4基因多态性与精神分裂症及阴性和一般精神病理症状显著关联。展开更多
目的探讨G蛋白偶联受体48(G protein coupled receptor 48,GPR48)和Toll样受体4(toll like receptor 4,TLR4)在大鼠激素性股骨头坏死组织中的表达变化及其相关性,阐明GPR48与TLR4在激素性股骨头坏死进程中的调控作用。方法健康SD大鼠68...目的探讨G蛋白偶联受体48(G protein coupled receptor 48,GPR48)和Toll样受体4(toll like receptor 4,TLR4)在大鼠激素性股骨头坏死组织中的表达变化及其相关性,阐明GPR48与TLR4在激素性股骨头坏死进程中的调控作用。方法健康SD大鼠68只,按随机数字表法分为实验组(44只,肌注地塞米松10 mg/kg,每周1次)和对照组(24只,注射等体积生理盐水代替激素),于2、6、8、10周时分别获取实验组11只和对照组6只大鼠的双侧股骨头,进行组织学观察(HE、Masson三色染色)、免疫组化及RT-PCR检测。结果①HE染色显示,实验组2周可见骨小梁微小断裂,10周骨小梁断裂范围扩大,形成大量空骨陷窝;Masson三色染色及胶原含量分析显示,与对照组相比,实验组单位面积内胶原含量从第6周开始下降,第10周明显下降,差异有统计学意义(P<0.05),实验组组内各个时间点的比较差异有统计学意义(P<0.05),胶原含量呈逐渐下降趋势;②免疫组织化学染色分析:实验组6、8、10周各个时间点GPR48表达的累积光密度(5.87±0.53)、(4.82±0.52)、(3.94±0.49)均低于对照组(P<0.05),并呈逐渐降低趋势;实验组6、8、10周各个时间点TLR4表达的累积光密度(6.93±0.58)、(8.10±0.46)、(8.70±0.95)均高于对照组(P<0.05),并呈逐渐上升趋势;实验组不同时间点GPR48与TLR4表达呈负相关(r=-0.821,P<0.05);③RT-PCR检测:GPR48 mRNA表达在实验组内各个时间点之间呈逐渐降低趋势,而TLR4 mRNA表达则呈逐渐上升趋势;实验组不同时间点GPR48与TLR4 mRNA表达呈负相关(r=-0.791,P<0.05)。结论大鼠激素性股骨头坏死发展进程中GPR48和TLR4的表达呈负相关性,共同介导坏死过程中的炎症免疫反应。展开更多
基金National Nature Science Foundation for Distinguished Young Scholar of China (No.39525022)National Basic Research Program(No.2004CB518703) National Nature Science Foundation of China (No.30570085).
文摘Latent membrane protein 1 （LMP1）, an important protein encoded by Epstein Barr virus （EBV）, has been implied to link with the pathogenesis of nasopharyngeal carcinoma （NPC）. Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMPI. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC.
文摘目的：探讨G-蛋白信号转导调节子4（regulator of G-protein signaling-4，RGS4）基因与精神分裂症及临床症状的遗传关联。方法：应用病例对照关联研究设计，采用聚合酶链式反应-限制性片断长度多态性（PCR-RFLP）和DNA测序方法，分析386例精神分裂症患者和390例正常对照者中RGS4基因4个单核苷酸多态性（SNP）位点与精神分裂症的关联。并采用阳性和阴性症状量表（PANSS）评估患者的临床症状，进一步分析PANSS因子分与RGS4多态性的关联。结果：RGS4基因的两个多态性位点rs12753561（T〉G，χ^2=8．970，P=0．002）和rs10759（C〉A，χ^2=13．773，F=0．002，P=0．002）与精神分裂症关联，由上述4个SNPs组成的多个单体型如AAGA（χ^2=11．120，P=0．0008，OR=0．52，95％CI=0．36—0．77）和GGGC（χ^2=10．096，P=0．001，OR=1．43，95％CI=1．15—1．79）均与精神分裂症关联。PANSS量表的阴性症状因子分与rs12753561（t=2．216，P=0．029）和rs10759（t=2．543，P=0．012）关联。结论：RGS4基因多态性与精神分裂症及阴性和一般精神病理症状显著关联。
