Objective To evaluate the effect of neuropathic pain on the expression of GABAB receptor subtypes in spinal cord of rats with chronic constriction injury.Methods 36 rats were divided into Sham group and CCI group resp...Objective To evaluate the effect of neuropathic pain on the expression of GABAB receptor subtypes in spinal cord of rats with chronic constriction injury.Methods 36 rats were divided into Sham group and CCI group respectively.In CCI group,the left common sciatic nerve of anesthetized rats was exposed,four silk ligatures（30） were tied loosely proximal to the sciatica’s trifurcation.In Sham group,Sham surgery was undergone by exposing the left sciatic nerve without ligation.Hind paw thermal withdrawl latency（TWL） to thermal stimulus and mechanical withdrawl threshold（MWT） of mechanical stimulus were recorded before ligation or on 1 d,3 d,5 d,7 d,10 d,14 d,21 d,28 d after ligation respectively（n=8）.Expression of GABAB receptor subtypes were detected before ligation and 1 d,3 d,7 d,14 d,28 d after ligation respectively using bothe immunohistochemical assay（n=6） and Western blot technique（n=4）.Results There were no significant differences on the values of MWT and TWL before ligation between Sham group and CCI group.Compared with Sham group,the MWT and TWL of rats in CCI group significantly decreased after ligation（P<0.01,P<0.05） and continued to be decreased until 28 d after ligation.Both immunoreactive neurons and protein content of GABAB1a or GABAB2 subtypes increased on the 3rd day after ligation,then both decreased on the 7th day after ligation,and lasted for 28 dafter ligation.（P<0.01,P<0.05）.There were no changes of GABAB1b after ligation（P>0.05）.Conclusion There existed differences among expression of spinal GABAB receptor subtypes on rats with chronic constriction injury induced neuropathic pain.The expression of spinal GABAB1a and GABAB2 decreased while there were no changes of GABAB1b on neuropathic rats.展开更多
Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body w...Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.展开更多
文摘Objective To evaluate the effect of neuropathic pain on the expression of GABAB receptor subtypes in spinal cord of rats with chronic constriction injury.Methods 36 rats were divided into Sham group and CCI group respectively.In CCI group,the left common sciatic nerve of anesthetized rats was exposed,four silk ligatures（30） were tied loosely proximal to the sciatica’s trifurcation.In Sham group,Sham surgery was undergone by exposing the left sciatic nerve without ligation.Hind paw thermal withdrawl latency（TWL） to thermal stimulus and mechanical withdrawl threshold（MWT） of mechanical stimulus were recorded before ligation or on 1 d,3 d,5 d,7 d,10 d,14 d,21 d,28 d after ligation respectively（n=8）.Expression of GABAB receptor subtypes were detected before ligation and 1 d,3 d,7 d,14 d,28 d after ligation respectively using bothe immunohistochemical assay（n=6） and Western blot technique（n=4）.Results There were no significant differences on the values of MWT and TWL before ligation between Sham group and CCI group.Compared with Sham group,the MWT and TWL of rats in CCI group significantly decreased after ligation（P<0.01,P<0.05） and continued to be decreased until 28 d after ligation.Both immunoreactive neurons and protein content of GABAB1a or GABAB2 subtypes increased on the 3rd day after ligation,then both decreased on the 7th day after ligation,and lasted for 28 dafter ligation.（P<0.01,P<0.05）.There were no changes of GABAB1b after ligation（P>0.05）.Conclusion There existed differences among expression of spinal GABAB receptor subtypes on rats with chronic constriction injury induced neuropathic pain.The expression of spinal GABAB1a and GABAB2 decreased while there were no changes of GABAB1b on neuropathic rats.
文摘Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.
