Crosstalk between xenobiotic metabolism and energy metabolism in the liver has provided a potential opportunity to target xenobiotic receptors to treat metabolic diseases.Activation of constitutive andro stane recepto...Crosstalk between xenobiotic metabolism and energy metabolism in the liver has provided a potential opportunity to target xenobiotic receptors to treat metabolic diseases.Activation of constitutive andro stane receptor(CAR),a xenobiotic-sensing nuclear receptor,has been shown to inhibit obesity,suppress hepatic gluconeogenesis,and ameliorate hyperglycemia in rodent models of obesity and type 2 diabetes.However,the underlying molecular mechanism remains to be defined.The growth arrest and DNA damage-inducible gene 45 b(Gadd45 b),a well-known anti-apoptotic factor,has been shown to be an inducible coactivator of CAR in promoting rapid liver growth.It is unknown whether the effect of CAR on energy metabolism depends on GADD45 B.In the present study and by using a high fat diet(HFD)-induced obesity model,we show that reduced body weight gain and improved insulin sensitivity by the CAR agonist 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene(TCPOBOP)were markedly blunted in Gadd45 b knockout mice.Mechanistically,the TCPOBOP-responsive inhibition of hepatic lipogenesis,gluconeogenesis,and adipose inflammation observed in wild type mice were largely abolished in Gadd45 b knockout mice.We conclude that Gadd45 b is required in part for the metabolic benefits of CAR activation.展开更多
基金supported in part by the Joseph Koslow Endowed Professorship from the University of Pittsburgh School of Pharmacy(Pittsburgh,PA,USA)
文摘Crosstalk between xenobiotic metabolism and energy metabolism in the liver has provided a potential opportunity to target xenobiotic receptors to treat metabolic diseases.Activation of constitutive andro stane receptor(CAR),a xenobiotic-sensing nuclear receptor,has been shown to inhibit obesity,suppress hepatic gluconeogenesis,and ameliorate hyperglycemia in rodent models of obesity and type 2 diabetes.However,the underlying molecular mechanism remains to be defined.The growth arrest and DNA damage-inducible gene 45 b(Gadd45 b),a well-known anti-apoptotic factor,has been shown to be an inducible coactivator of CAR in promoting rapid liver growth.It is unknown whether the effect of CAR on energy metabolism depends on GADD45 B.In the present study and by using a high fat diet(HFD)-induced obesity model,we show that reduced body weight gain and improved insulin sensitivity by the CAR agonist 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene(TCPOBOP)were markedly blunted in Gadd45 b knockout mice.Mechanistically,the TCPOBOP-responsive inhibition of hepatic lipogenesis,gluconeogenesis,and adipose inflammation observed in wild type mice were largely abolished in Gadd45 b knockout mice.We conclude that Gadd45 b is required in part for the metabolic benefits of CAR activation.
