Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lili...Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.展开更多
Multiple genes and microRNAs(miRNAs)improve grain yield by promoting tillering.MiR319s are known to regulate several aspects of plant development;however,whether miR319s are essential for tillering regulation remains ...Multiple genes and microRNAs(miRNAs)improve grain yield by promoting tillering.MiR319s are known to regulate several aspects of plant development;however,whether miR319s are essential for tillering regulation remains unclear.Here,we report that miR319 is highly expressed in the basal part of rice plant at different development stages.The miR319 knockdown line Short Tandem Target Mimic 319(STTM319)showed higher tiller bud length in seedlings under low nitrogen(N)condition and higher tiller bud number under high N condition compared with the miR319a-overexpression line.Through targets prediction,we identified OsTCP21 and OsGAmyb as downstream targets of miR319.Moreover,OsTCP21 and OsGAmyb overexpression lines and STTM319 had increased tiller bud length and biomass,whereas both were decreased in OsTCP21 and OsGAmyb knockout lines and OE319a.These data suggest that miR319 regulates rice tiller bud development and tillering through targeting OsTCP21 and OsGAmyb.Notably,the tiller number and grain yield increased in STTM319 and overexpression lines of OsTCP21 and OsGAmyb but decreased in OE319a and knockout lines of OsTCP21 and OsGAmyb.Taken together,our findings indicate that miR319s negatively affect tiller number and grain yield by targeting OsTCP21 and OsGAmyb,revealing a novel function for miR319 in rice.展开更多
Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (9...Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up regulated by gibberellin (GA) and down regulated by abscisic acid (ABA). The tissue and developmental stage specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non detectable level soon after germination.展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200500)the Fundamental Research Funds for the Central Universities(KYZZ2022004)+1 种基金the Project for Crop Germplasm Resources Conservation of Jiangsu(2021-SJ-011)the High Level Talent Project of the Top Six Talents in Jiangsu(NY-077)。
文摘Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.
基金supported by the grant from the National Natural ScienceFoundation of China(31301250)the Talent Project from Guizhou Education Department(Qian jiao he KY zi(2021)024)+2 种基金the Wuhan Science and Technology Project(2020020601012259)the Key Cultivation Project of Guizhou University(201903)the Talent Project from Thousands of Innovative and Entrepreneurial in Guizhou Province。
文摘Multiple genes and microRNAs(miRNAs)improve grain yield by promoting tillering.MiR319s are known to regulate several aspects of plant development;however,whether miR319s are essential for tillering regulation remains unclear.Here,we report that miR319 is highly expressed in the basal part of rice plant at different development stages.The miR319 knockdown line Short Tandem Target Mimic 319(STTM319)showed higher tiller bud length in seedlings under low nitrogen(N)condition and higher tiller bud number under high N condition compared with the miR319a-overexpression line.Through targets prediction,we identified OsTCP21 and OsGAmyb as downstream targets of miR319.Moreover,OsTCP21 and OsGAmyb overexpression lines and STTM319 had increased tiller bud length and biomass,whereas both were decreased in OsTCP21 and OsGAmyb knockout lines and OE319a.These data suggest that miR319 regulates rice tiller bud development and tillering through targeting OsTCP21 and OsGAmyb.Notably,the tiller number and grain yield increased in STTM319 and overexpression lines of OsTCP21 and OsGAmyb but decreased in OE319a and knockout lines of OsTCP21 and OsGAmyb.Taken together,our findings indicate that miR319s negatively affect tiller number and grain yield by targeting OsTCP21 and OsGAmyb,revealing a novel function for miR319 in rice.
文摘Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up regulated by gibberellin (GA) and down regulated by abscisic acid (ABA). The tissue and developmental stage specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non detectable level soon after germination.
