[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expr...[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.展开更多
Holttumochloa has previously only been recorded from Malaysia. Here we describe and illustrate a new species, Holttumochloa hainanensis sp. nov., from the lowland montane forests of Diaoluo Mountain on the Island of H...Holttumochloa has previously only been recorded from Malaysia. Here we describe and illustrate a new species, Holttumochloa hainanensis sp. nov., from the lowland montane forests of Diaoluo Mountain on the Island of Hainan, South China. Morphologically, H. hainanensis is similar to Holttumochloa korbuensis,but can be clearly distinguished from it in having larger culms covered by white wax, longer leaf blades,larger pseudospikelets and anthers. Furthermore, molecular phylogeny based on the nuclear gene GBSSI corroborates the identification of the new species and its affinity. The biogeographical significance of the new record of Holttumochloa in South China is also highlighted in this study.展开更多
马铃薯是世界上主要粮食作物之一,其块茎中的贮藏淀粉是马铃薯的主要利用产物。PROTEIN TARGETING TO STARCH 1(PTST1)基因在直链淀粉合成中具有重要作用。然而,它在马铃薯中的功能尚不清楚。本文以马铃薯PTST1为目的基因,从基因特征、...马铃薯是世界上主要粮食作物之一,其块茎中的贮藏淀粉是马铃薯的主要利用产物。PROTEIN TARGETING TO STARCH 1(PTST1)基因在直链淀粉合成中具有重要作用。然而,它在马铃薯中的功能尚不清楚。本文以马铃薯PTST1为目的基因,从基因特征、表达模式及与GBSSI的互作关系方面进行了研究。PTST1蛋白由252个氨基酸残基组成,羧基端含有碳水化合物结合结构域。PTST1在多个组织的表达均低于GBSSI,在块茎发育时期,其表达没有明显的升高趋势。GBSSI在块茎中高表达,且随着块茎发育,其表达升高,说明PTST1与GBSSI的表达模式不同。酵母双杂交实验、烟草荧光素酶互补实验和双分子荧光互补实验证明PTST1与GBSSI蛋白互作,且互作发生于叶绿体基质。上述结果表明PTST1可能与叶片和块茎中直链淀粉的合成有密切的关系。本研究为调控马铃薯直链淀粉合成提供了新的基因资源。展开更多
基金Supported by the National Natural Science Foundation Program(30160010)~~
文摘[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.
基金supported by the National Natural Science Foundation of China (grants 31430011 and 31670396)
文摘Holttumochloa has previously only been recorded from Malaysia. Here we describe and illustrate a new species, Holttumochloa hainanensis sp. nov., from the lowland montane forests of Diaoluo Mountain on the Island of Hainan, South China. Morphologically, H. hainanensis is similar to Holttumochloa korbuensis,but can be clearly distinguished from it in having larger culms covered by white wax, longer leaf blades,larger pseudospikelets and anthers. Furthermore, molecular phylogeny based on the nuclear gene GBSSI corroborates the identification of the new species and its affinity. The biogeographical significance of the new record of Holttumochloa in South China is also highlighted in this study.
文摘马铃薯是世界上主要粮食作物之一,其块茎中的贮藏淀粉是马铃薯的主要利用产物。PROTEIN TARGETING TO STARCH 1(PTST1)基因在直链淀粉合成中具有重要作用。然而,它在马铃薯中的功能尚不清楚。本文以马铃薯PTST1为目的基因,从基因特征、表达模式及与GBSSI的互作关系方面进行了研究。PTST1蛋白由252个氨基酸残基组成,羧基端含有碳水化合物结合结构域。PTST1在多个组织的表达均低于GBSSI,在块茎发育时期,其表达没有明显的升高趋势。GBSSI在块茎中高表达,且随着块茎发育,其表达升高,说明PTST1与GBSSI的表达模式不同。酵母双杂交实验、烟草荧光素酶互补实验和双分子荧光互补实验证明PTST1与GBSSI蛋白互作,且互作发生于叶绿体基质。上述结果表明PTST1可能与叶片和块茎中直链淀粉的合成有密切的关系。本研究为调控马铃薯直链淀粉合成提供了新的基因资源。
