Growth and Differentiation Factor-8(GDF-8) is a new member of TGF-β super-family.It has been shown that GDF-8 is specifically expressed in skeleton muscle in mouse and its function is to inhibit the growth of muscle ...Growth and Differentiation Factor-8(GDF-8) is a new member of TGF-β super-family.It has been shown that GDF-8 is specifically expressed in skeleton muscle in mouse and its function is to inhibit the growth of muscle cell,so it is named as Myostatin.Here,we amplified 3′half-length GDF-8 cDNA from chicken skeleton muscle by RT-PCR,and cloned it into the prokaryotic expression vector pTrcHisB,which was then transformed into E.coli Top10 cells.The recombinant 6×His-GDF-8 fusion protein expressed in the Top10 cells was purified by Ni+-Affinity Chromatography for future study.展开更多
研究不同pH值下,口服靶向FOXO1与GDF-8基因反义RNA寡核苷酸药物对药效的影响。化学合成靶向FOXO1与GDF-8基因的有效反义RNA寡核苷酸片段,调节药物PH值,通过灌胃方式给药,给药20 d后处死小鼠,进行体重,腿部肌肉增长情况分析。提取腿部肌...研究不同pH值下,口服靶向FOXO1与GDF-8基因反义RNA寡核苷酸药物对药效的影响。化学合成靶向FOXO1与GDF-8基因的有效反义RNA寡核苷酸片段,调节药物PH值,通过灌胃方式给药,给药20 d后处死小鼠,进行体重,腿部肌肉增长情况分析。提取腿部肌肉组织总RNA,用real time PCR检测FOXO1与GDF-8基因的表达。结果表明,服用RNA oligos的试验组小鼠腿部肌肉重量均比对照组肌肉重量增长快。其中pH为7.0的RNA oligos的效果比pH为5.0的效果好,pH9.0的RNAoligos的作用效果最弱。与小鼠体重变化结果一致,real time PCR检测实验组FOXO1与GDF-8基因转录水平较对照组均有明显下降,其中服用pH为7.0的RNA oligos的下降最多。因此,口服靶向FOXO1与GDF-8基因的反义RNA寡核苷酸药物的最适pH值应为中性偏酸。展开更多
文摘Growth and Differentiation Factor-8(GDF-8) is a new member of TGF-β super-family.It has been shown that GDF-8 is specifically expressed in skeleton muscle in mouse and its function is to inhibit the growth of muscle cell,so it is named as Myostatin.Here,we amplified 3′half-length GDF-8 cDNA from chicken skeleton muscle by RT-PCR,and cloned it into the prokaryotic expression vector pTrcHisB,which was then transformed into E.coli Top10 cells.The recombinant 6×His-GDF-8 fusion protein expressed in the Top10 cells was purified by Ni+-Affinity Chromatography for future study.
文摘研究不同pH值下,口服靶向FOXO1与GDF-8基因反义RNA寡核苷酸药物对药效的影响。化学合成靶向FOXO1与GDF-8基因的有效反义RNA寡核苷酸片段,调节药物PH值,通过灌胃方式给药,给药20 d后处死小鼠,进行体重,腿部肌肉增长情况分析。提取腿部肌肉组织总RNA,用real time PCR检测FOXO1与GDF-8基因的表达。结果表明,服用RNA oligos的试验组小鼠腿部肌肉重量均比对照组肌肉重量增长快。其中pH为7.0的RNA oligos的效果比pH为5.0的效果好,pH9.0的RNAoligos的作用效果最弱。与小鼠体重变化结果一致,real time PCR检测实验组FOXO1与GDF-8基因转录水平较对照组均有明显下降,其中服用pH为7.0的RNA oligos的下降最多。因此,口服靶向FOXO1与GDF-8基因的反义RNA寡核苷酸药物的最适pH值应为中性偏酸。
