Mapmaker3.0和作图软件使用

构建各种生物体的遗传连锁图谱成为分子遗传学和作物育种领域QTL定位的热点。Mapmaker3.0是当前普遍用于遗传连锁距离计算的软件。Mapmaker3.0的使用首先要准备标记数据文件,然后用Sequence命令输入欲分析的标记座位,接着键入Group命令指导程序将Sequence中的标记分为不同的连锁群,接下来,程序在一个连锁群内部决定最有可能的标记排列次序,计算一个连锁群中所有的标记每一个可能的次序最大似然图谱,比较这些图谱的可能性并找出最合适图谱,这部分工作用Compare命令完成,再用sequence和map命令得到标记之间的连锁数据。之后,将这些数据按Genemap或MapDraw分析软件所要求的数据格式输入,通过对这2个软件的正确使用,就可得到需要的遗传连锁图谱图形文件。

Morphological Analysis and Gene Mapping of a Rice Dwarf Mutant

[Objective] This study aimed to conduct morphological analysis and gene mapping of a rice dwarf mutant. [Method] A dwarf mutant （Xiaoxiang＇ai） was used as test material for morphological observation. Xiaoxiang＇ai was used as female parent and semi-dwarf material Xiangzao143 was used as male parent to construct populations for genetic analysis. Gene mapping was conducted by using micro- satellite markers. [Result] The average height of Xiaoxiang＇ai was 55 cm; genetic analysis results showed that plant height of F1 individuals was similar with the male parent, while semi-dwarf plants and dwarf plants were observed in F2 populations and the segregation ratio was nearly 3：1, indicating that plant height trait of Xiaoxi- ang＇ai is mainly controlled by one pair of recessive gene located on rice chromo- some 5 and in the upstream of RM249, with a genetic distance of 8.4 cM. [Conclu- sion] rRH is a new dwarf gene in rice.

基于GEOWAY GeneMap模块的地图缩编方法研究

通过缩编来形成中小比例尺数据相对于其他数据生产方式具有周期短、成本低的特点,是未来地理信息数据库建设、维护和更新的主要技术手段。以1∶2000数字化地形图缩编1∶5000 GIS数据为例探讨利用GEOWAY GeneMap地图缩编模块进行地图缩编的方法。

Auto CAD与GENEMAP图形数据转换

本文就GENEMAP对数字化地形图的处理方法加以分析、探讨,提出用Auto CAD12.0进行数字化成图,然后再转换到GENEMAP中去,在数字化操作和软件编制时应注意的几个事项。