A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the ...A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the new compound against Peronophythora litchii was assessed by microplate Alamar blue assay (MABA).展开更多
This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by cultu...This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by culture of dialysis fluid. The purpose of this report is to provide data on a fungal peritonitis due to a non-common agent.展开更多
The coevolution and coexistence of bacterial–fungal consortium have been widely reported in various natural ecosystems. The transboundary communication mediated by bacterial acyl–homoserine lactone signals probably ...The coevolution and coexistence of bacterial–fungal consortium have been widely reported in various natural ecosystems. The transboundary communication mediated by bacterial acyl–homoserine lactone signals probably is the driving force of fungal spore germination. This study aimed to report a functional bacterial signal molecule, C10-acyl homoserine lactone, which could be sensed by Galactomyces geotrichum. The spore germination rates of G. geotrichum increased by 22%. Meanwhile, carbohydrate production improved by 1.0-to 2.5-fold. G. geotrichum signaled to C10-HSL through receptor gene Rho1and made a response in cell wall assembly and carbohydrate biosynthesis by the upregulated expression(above 1-fold) of functional genes, such as Smi1, Utr2, and Chs2. It contributed to spore germination and morphology transformation together. This study provides a novel perspective for understating the transboundary cooperation between fungi and bacteria by cell-to-cell communication.展开更多
Bioreduction of some β-carbonyl phenyl sulfides, sulfoxides and sulfones (1, 3, 5, 8, 11 and 13) by Geotrichum sp. was studied. Reduction of β-carbonyl phenyl sulfoxides (5 and 8) gave anti-Prelog sulfoxide alcohols...Bioreduction of some β-carbonyl phenyl sulfides, sulfoxides and sulfones (1, 3, 5, 8, 11 and 13) by Geotrichum sp. was studied. Reduction of β-carbonyl phenyl sulfoxides (5 and 8) gave anti-Prelog sulfoxide alcohols. (S,Ss)-3-Chloro-1-phenylsulfinylpropan-2-ol (9) WBP obtained in high yield with 95% e. e. after recrystallization from methylene chloride petroleum ether.展开更多
从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%...从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%、99.50%;平均核酸一致性分析,相似度分别为98.66%、98.65%、98.68%。结合形态特征和26S rDNA序列同源性分析,K2、Z7-1和Z8-43株菌均鉴定为白地霉。利用基因本体论、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源簇等数据库对白地霉K2进行基因功能注释,注释基因中40.74%与细胞组成相关,47.54%与生物过程相关,11.73%与分子功能相关。KEGG代谢途径分析发现萜类合成的相关基因,白地霉K2具有合成挥发性香味成分的遗传基础。采用气相色谱-质谱联用仪对白地霉K2菌株的发酵液挥发性成分进行检测分析,共检出50种挥发性成分,其中3-蒈烯、α-蒎烯和对伞花烃呈花香,L-α-松油醇呈松木香,4-萜烯醇、(-)-4-萜品醇呈丁香味,乙酸α-松油酯呈清甜香。推测白地霉是开菲尔酸奶香味物质的重要贡献菌株。本研究为白地霉K2用于发酵乳制品及其他发酵食品提供了理论基础。展开更多
文摘A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the new compound against Peronophythora litchii was assessed by microplate Alamar blue assay (MABA).
文摘This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by culture of dialysis fluid. The purpose of this report is to provide data on a fungal peritonitis due to a non-common agent.
基金the National Natural Science Foundation of China (Nos. 52070036, U20A20322)the Fundamental Research Funds for the Central Universities (No. 2412018ZD042) for their financial support。
文摘The coevolution and coexistence of bacterial–fungal consortium have been widely reported in various natural ecosystems. The transboundary communication mediated by bacterial acyl–homoserine lactone signals probably is the driving force of fungal spore germination. This study aimed to report a functional bacterial signal molecule, C10-acyl homoserine lactone, which could be sensed by Galactomyces geotrichum. The spore germination rates of G. geotrichum increased by 22%. Meanwhile, carbohydrate production improved by 1.0-to 2.5-fold. G. geotrichum signaled to C10-HSL through receptor gene Rho1and made a response in cell wall assembly and carbohydrate biosynthesis by the upregulated expression(above 1-fold) of functional genes, such as Smi1, Utr2, and Chs2. It contributed to spore germination and morphology transformation together. This study provides a novel perspective for understating the transboundary cooperation between fungi and bacteria by cell-to-cell communication.
基金Project supported by the National Natural Science Foundation of China (No. 29790125).
文摘Bioreduction of some β-carbonyl phenyl sulfides, sulfoxides and sulfones (1, 3, 5, 8, 11 and 13) by Geotrichum sp. was studied. Reduction of β-carbonyl phenyl sulfoxides (5 and 8) gave anti-Prelog sulfoxide alcohols. (S,Ss)-3-Chloro-1-phenylsulfinylpropan-2-ol (9) WBP obtained in high yield with 95% e. e. after recrystallization from methylene chloride petroleum ether.
文摘从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%、99.50%;平均核酸一致性分析,相似度分别为98.66%、98.65%、98.68%。结合形态特征和26S rDNA序列同源性分析,K2、Z7-1和Z8-43株菌均鉴定为白地霉。利用基因本体论、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源簇等数据库对白地霉K2进行基因功能注释,注释基因中40.74%与细胞组成相关,47.54%与生物过程相关,11.73%与分子功能相关。KEGG代谢途径分析发现萜类合成的相关基因,白地霉K2具有合成挥发性香味成分的遗传基础。采用气相色谱-质谱联用仪对白地霉K2菌株的发酵液挥发性成分进行检测分析,共检出50种挥发性成分,其中3-蒈烯、α-蒎烯和对伞花烃呈花香,L-α-松油醇呈松木香,4-萜烯醇、(-)-4-萜品醇呈丁香味,乙酸α-松油酯呈清甜香。推测白地霉是开菲尔酸奶香味物质的重要贡献菌株。本研究为白地霉K2用于发酵乳制品及其他发酵食品提供了理论基础。
