The aim of this study was to investigate the effect of 30 min forebrain ischemia, followed by 120 min reperfusion on extracellular fluid (ECF) levels of dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their m...The aim of this study was to investigate the effect of 30 min forebrain ischemia, followed by 120 min reperfusion on extracellular fluid (ECF) levels of dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites, homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the striatum of gerbils, so as to obtain further information on the mechanism of Radix Salviae Miltiorrhizae (RSM)-induced neuroprotection. Microdialysis was used to sample the extracellular space. Dialysate was measured by high performance liquid chromatography with electrochemical detector (HPLC-ED). ECF DA, NE levels increased from basal levels by 282, 227 and 221 folds, by 9.14, 8.51 and 8.25 folds, respectively for the three ischemic duration (0-10; 11-20; 21-30 min). ECF DA, NE, 5-HT levels in the RSM-treated group were significantly decreased as compared with those in the control group during ischemia (P展开更多
Objective\ Exploring the alteration of glucocorticoid receptor (GCR) following forebrain ischemia in gerbils. Methods\ Establish a brain ischemia model by occluding bilateral common carotid. The GCR concentrations in ...Objective\ Exploring the alteration of glucocorticoid receptor (GCR) following forebrain ischemia in gerbils. Methods\ Establish a brain ischemia model by occluding bilateral common carotid. The GCR concentrations in forebrain tissue cytosol were detected by radioligand binding assay. The plasma concentrations of cortisol were determined by the method of competitive protein binding analysis. Results\ GCR concentrations had no significant change after 10 min ischemia (P>0 05), while decreased significantly in 1 h ischemia (P<0.01), and reduced more severely in 1 h ischemia followed by 3 h reperfusion (P<0.01). Plasma cortisol increased markedly in 10 min ischemia and 1 h ischemia (P<0.001), while decreased in 1 h ischemia followed by 3 h reperfusion. Conclusion\ GCR concentrations in brain decreased following forebrain ischemia in gerbil.展开更多
OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices fr...OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 micro mol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion. RESULTS: Caffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P展开更多
文摘The aim of this study was to investigate the effect of 30 min forebrain ischemia, followed by 120 min reperfusion on extracellular fluid (ECF) levels of dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites, homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the striatum of gerbils, so as to obtain further information on the mechanism of Radix Salviae Miltiorrhizae (RSM)-induced neuroprotection. Microdialysis was used to sample the extracellular space. Dialysate was measured by high performance liquid chromatography with electrochemical detector (HPLC-ED). ECF DA, NE levels increased from basal levels by 282, 227 and 221 folds, by 9.14, 8.51 and 8.25 folds, respectively for the three ischemic duration (0-10; 11-20; 21-30 min). ECF DA, NE, 5-HT levels in the RSM-treated group were significantly decreased as compared with those in the control group during ischemia (P
文摘Objective\ Exploring the alteration of glucocorticoid receptor (GCR) following forebrain ischemia in gerbils. Methods\ Establish a brain ischemia model by occluding bilateral common carotid. The GCR concentrations in forebrain tissue cytosol were detected by radioligand binding assay. The plasma concentrations of cortisol were determined by the method of competitive protein binding analysis. Results\ GCR concentrations had no significant change after 10 min ischemia (P>0 05), while decreased significantly in 1 h ischemia (P<0.01), and reduced more severely in 1 h ischemia followed by 3 h reperfusion (P<0.01). Plasma cortisol increased markedly in 10 min ischemia and 1 h ischemia (P<0.001), while decreased in 1 h ischemia followed by 3 h reperfusion. Conclusion\ GCR concentrations in brain decreased following forebrain ischemia in gerbil.
文摘OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 micro mol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion. RESULTS: Caffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P
