The phytohormone auxin plays a central role in coordinating plant growth and development.GH3 is one of the three gene families that respond rapidly during auxin stimulation.Here,we report the identification and charac...The phytohormone auxin plays a central role in coordinating plant growth and development.GH3 is one of the three gene families that respond rapidly during auxin stimulation.Here,we report the identification and characterization of the GH3 gene family in maize.A total of 12 GH3 genes were identified,which are not evenly distributed over the 10 maize chromosomes.Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein.Diversified c/＇s-elements were found in promoters of maize GH3 genes.In this study,the 12 maize GH3 proteins were primarily classified into two phylogenetic groups,similar to the 13 rice GH3 proteins,while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group.Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated.Additionally,maize GH3 genes displayed variable changes at transcript level upon pathogen infection.Results presented here provide insight into the diversification and evolution of GH3 proteins,and lay a foundation for the functional characterization of these GH3 genes in future,especially for elucidating the mechanisms of GH3-mediated pathogenesis.展开更多
Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many...Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many biology processes. Studies have demonstrated GH3 gene family in various plants, but no ge-nome -wide analysis of the GH3 gene family has been conducted for a legume species. Here we performed a comprehensive genome mining and identified a total of 24 GH3 genes, located on 13 different chromo-somes in the soybean genome （ GmGH3. 1 to GmGH3. 24） . Most of the predicted GmGH3s clustered to-gether in pairs, reflecting the ancient genome duplication event. Analysis of gene structure revealed the presence of introns in protein - coding regions, and that their size, abundance and distribution varied within the gene family. Expression analysis of GmGH3 genes in soybean various tissues of different devel-opmental stages displayed their temporally and spatially expression patterns. We have identified 24 GH3 genes in the soybean genome. The expansion of GmGH3 genes and their distribution pattern on the chro-mosomes revealed genome - wide segmental duplications of soybean. Diverse expression of GmGH3s dur-ing soybean growth and development indicated their important function roles.展开更多
基金financially supported by the National Natural Science Foundation of China (31371625)
文摘The phytohormone auxin plays a central role in coordinating plant growth and development.GH3 is one of the three gene families that respond rapidly during auxin stimulation.Here,we report the identification and characterization of the GH3 gene family in maize.A total of 12 GH3 genes were identified,which are not evenly distributed over the 10 maize chromosomes.Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein.Diversified c/＇s-elements were found in promoters of maize GH3 genes.In this study,the 12 maize GH3 proteins were primarily classified into two phylogenetic groups,similar to the 13 rice GH3 proteins,while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group.Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated.Additionally,maize GH3 genes displayed variable changes at transcript level upon pathogen infection.Results presented here provide insight into the diversification and evolution of GH3 proteins,and lay a foundation for the functional characterization of these GH3 genes in future,especially for elucidating the mechanisms of GH3-mediated pathogenesis.
基金This work was supported by the National Natural Science Foundation of China [ grant number. 31371654, 31522042, 31501655 and 31501334]； National Trans-genic Project [ grant number. 2014ZX08004003, 2015ZX08004003 and 2016ZX08004003 ]；Agricultur-al Science and Technology Innovation Program, Breed-ing project [grant number. SQ2016ZY03002375 ] ； Wuhan Chenguang Plan [grant number. 2015070404-010193].
文摘Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many biology processes. Studies have demonstrated GH3 gene family in various plants, but no ge-nome -wide analysis of the GH3 gene family has been conducted for a legume species. Here we performed a comprehensive genome mining and identified a total of 24 GH3 genes, located on 13 different chromo-somes in the soybean genome （ GmGH3. 1 to GmGH3. 24） . Most of the predicted GmGH3s clustered to-gether in pairs, reflecting the ancient genome duplication event. Analysis of gene structure revealed the presence of introns in protein - coding regions, and that their size, abundance and distribution varied within the gene family. Expression analysis of GmGH3 genes in soybean various tissues of different devel-opmental stages displayed their temporally and spatially expression patterns. We have identified 24 GH3 genes in the soybean genome. The expansion of GmGH3 genes and their distribution pattern on the chro-mosomes revealed genome - wide segmental duplications of soybean. Diverse expression of GmGH3s dur-ing soybean growth and development indicated their important function roles.
