In order to investigate the relationship between abnormal intracellular signal transduction and tumorgenesis of human pituitary somatotrophinomas, the effects of protein kinase A (PKA) dependent growth hormone (GH) r...In order to investigate the relationship between abnormal intracellular signal transduction and tumorgenesis of human pituitary somatotrophinomas, the effects of protein kinase A (PKA) dependent growth hormone (GH) releasing hormone (GHRH) and protein kinase C (PKC) dependent GH releasing peptide (GHRP 6) on cAMP production were observed by using cell culture and biochemical methods, and the expression of the gsp oncogene was detected by using PCR and direct sequence assay methods in 11 patients with human pituitary somatotrophinomas. It was found that GHRP 6 exerted significant stimulatory effect on cAMP production by 2 gsp positive tumors and no effect on the gsp negative tumors. GHRP 6 could enhance the stimulation of cAMP production induced by GHRH in tumor without gsp oncogenes. It was suggested that both GHRH and GHRP 6 exert identical effects on human pituitary soamtotrophinomas, which was contributed to the cross talk between the two intracellular signal transduction pathways in pituitary cells.展开更多
基金This project was supported by the grants from National Natural Science Foundation of China (No. 396 70 736 ) Deutsche Gesel
文摘In order to investigate the relationship between abnormal intracellular signal transduction and tumorgenesis of human pituitary somatotrophinomas, the effects of protein kinase A (PKA) dependent growth hormone (GH) releasing hormone (GHRH) and protein kinase C (PKC) dependent GH releasing peptide (GHRP 6) on cAMP production were observed by using cell culture and biochemical methods, and the expression of the gsp oncogene was detected by using PCR and direct sequence assay methods in 11 patients with human pituitary somatotrophinomas. It was found that GHRP 6 exerted significant stimulatory effect on cAMP production by 2 gsp positive tumors and no effect on the gsp negative tumors. GHRP 6 could enhance the stimulation of cAMP production induced by GHRH in tumor without gsp oncogenes. It was suggested that both GHRH and GHRP 6 exert identical effects on human pituitary soamtotrophinomas, which was contributed to the cross talk between the two intracellular signal transduction pathways in pituitary cells.
