The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolat...The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.展开更多
The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolat...The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.展开更多
[Objectives] This study aimed to determine the infection pathway and target organs of Streptococcus agalactiae in GIFT strain of Nile tilapia, thus providing theoretical basis for the breeding of disease-resistant til...[Objectives] This study aimed to determine the infection pathway and target organs of Streptococcus agalactiae in GIFT strain of Nile tilapia, thus providing theoretical basis for the breeding of disease-resistant tilapia and development of S. agalactiae vaccines.[Methods] GIFT strain of Nile tilapia was inoculated by S. agalactiae through intraperitoneal injection, oral gavage and in vitro immersion. The gill, spleen, liver and small intestine tissues of infected tilapia were collected for pathomorphological observation. Immunohistochemical localization was conducted using rabbit anti- S. agalactiae serum to identify the distribution pattern of S. agalactiae in various tissues of tilapia and its target organs via different infection pathways.[Results] GIFT strain of Nile tilapia could be infected by S. agalactiae via three artificial inoculation modes. Specifically, pathological changes occurred at 2 h post-inoculation in intraperitoneal injection and oral gavage groups, whereas tilapia in in vitro immersion group showed pathological changes at 5 h post-inoculation, and the lesion intensity in in vitro immersion group was slighter than that in intraperitoneal injection and oral gavage groups. Immunohistochemical localization indicated that the appearance time of positive signals in intraperitoneal injection group demonstrated an order of spleen→liver and gill→small intestine; positive signals in oral gavage group appeared in the order of small intestine→gill and spleen→liver; the appearance time of positive signals in in vitro immersion group showed an order of gill→spleen→liver and small intestine.[Conclusions] GIFT strain of Nile tilapia could be infected by S. agalactiae via intraperitoneal injection, oral gavage and in vitro immersion. The corresponding positive signals for pathogen infection were preferentially present in the spleen, intestine and gill tissues. Thus, preventing S. agalactiae contamination in aquaculture water and food sources is an effective measure to control the outbreak of S. agalactiae infections in tilapia under natural aquaculture conditions.展开更多
研究所用60个家系的吉富品系尼罗罗非鱼(GIFT strain Nile tilapia,Oreochromis niloticus;简称吉富罗非鱼)样本均为由世界渔业中心所在地马来西亚引进的原种。采用传统形态学测量方法,测量每尾鱼的体质量、体长、体高、体宽、头长、尾...研究所用60个家系的吉富品系尼罗罗非鱼(GIFT strain Nile tilapia,Oreochromis niloticus;简称吉富罗非鱼)样本均为由世界渔业中心所在地马来西亚引进的原种。采用传统形态学测量方法,测量每尾鱼的体质量、体长、体高、体宽、头长、尾柄长和尾柄高后进行主成分分析和相关分析,研究吉富品系尼罗罗非鱼群体内的形态差异。结果显示,吉富罗非鱼体高与体质量的相关性最大(R2=0.9002)。在体长与体质量对应的点状分布中,总群体、雌雄群体和每个家系群体中均呈现出2条带状,分布于2条带的个体间质量差异不显著(P>0.05),对应的个体数量之比接近常数0.7;体高、体宽、头长、尾柄长和尾柄高相应的分布则呈现出变异幅度迥异的条带。本研究表明,吉富罗非鱼群体内部在形态上存在差异。展开更多
用投喂添加色素的膨化颗粒饲料和观测排便相结合的方法,研究吉富品系尼罗罗非鱼(Genetic Improvement of Farmed Tilapias;GIFT)的摄食节律。结果发现,6个家系的吉富品系尼罗罗非鱼摄食具有明显的节律性,家系之间的摄食周期存在非常显...用投喂添加色素的膨化颗粒饲料和观测排便相结合的方法,研究吉富品系尼罗罗非鱼(Genetic Improvement of Farmed Tilapias;GIFT)的摄食节律。结果发现,6个家系的吉富品系尼罗罗非鱼摄食具有明显的节律性,家系之间的摄食周期存在非常显著差异(P<0.0001);t检验发现,各家系内摄食周期和排便周期的差异均不显著(P≥0.1097)。回归分析发现,摄食周期对生长无显著的影响(P=0.8988),但与摄食量之间存在显著的线性关系(R2=0.9679,P=0.0004)。对摄食节律聚类分析,6个家系可分为(1、2、6)、(3、4)、5共3个家系类别。该项研究能为GIFT的养殖投饵和进一步选育提供理论依据,为鱼类摄食节律研究提供方法上的参考。展开更多
基金the Natural Science Foundation of Shanxi Province,China (2009021038)the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003)+2 种基金the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005)the Nonprofit Research Institutes,Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01)the Scientific Research and Technological Development Projects of Nanning City(200801016B)
文摘The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.
基金the Natural Science Foundation of Shanxi Province,China(2009021038)the Doctor Initial Foundation of Shanxi Agricultural University(XB2009003)+2 种基金the Science and Technology Innovational Foundation of Shanxi Agricultural University(2009005)the Nonprofit Research Institutes,Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01)the Scientific Research and Technological Development Projects of Nanning City(200801016B)。
文摘The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.
基金Supported by Science and Technology Major Project of Guangxi(GK AA17204080-2)Guangxi Key Research and Development Program(GK AB16380077)+1 种基金Freshwater Fishery Industry Technology System of China(CARS-46)Aquathe Disease Resistance Breeding Project of Natinal Blue Granary plan(2019-2023)
文摘[Objectives] This study aimed to determine the infection pathway and target organs of Streptococcus agalactiae in GIFT strain of Nile tilapia, thus providing theoretical basis for the breeding of disease-resistant tilapia and development of S. agalactiae vaccines.[Methods] GIFT strain of Nile tilapia was inoculated by S. agalactiae through intraperitoneal injection, oral gavage and in vitro immersion. The gill, spleen, liver and small intestine tissues of infected tilapia were collected for pathomorphological observation. Immunohistochemical localization was conducted using rabbit anti- S. agalactiae serum to identify the distribution pattern of S. agalactiae in various tissues of tilapia and its target organs via different infection pathways.[Results] GIFT strain of Nile tilapia could be infected by S. agalactiae via three artificial inoculation modes. Specifically, pathological changes occurred at 2 h post-inoculation in intraperitoneal injection and oral gavage groups, whereas tilapia in in vitro immersion group showed pathological changes at 5 h post-inoculation, and the lesion intensity in in vitro immersion group was slighter than that in intraperitoneal injection and oral gavage groups. Immunohistochemical localization indicated that the appearance time of positive signals in intraperitoneal injection group demonstrated an order of spleen→liver and gill→small intestine; positive signals in oral gavage group appeared in the order of small intestine→gill and spleen→liver; the appearance time of positive signals in in vitro immersion group showed an order of gill→spleen→liver and small intestine.[Conclusions] GIFT strain of Nile tilapia could be infected by S. agalactiae via intraperitoneal injection, oral gavage and in vitro immersion. The corresponding positive signals for pathogen infection were preferentially present in the spleen, intestine and gill tissues. Thus, preventing S. agalactiae contamination in aquaculture water and food sources is an effective measure to control the outbreak of S. agalactiae infections in tilapia under natural aquaculture conditions.
文摘研究所用60个家系的吉富品系尼罗罗非鱼(GIFT strain Nile tilapia,Oreochromis niloticus;简称吉富罗非鱼)样本均为由世界渔业中心所在地马来西亚引进的原种。采用传统形态学测量方法,测量每尾鱼的体质量、体长、体高、体宽、头长、尾柄长和尾柄高后进行主成分分析和相关分析,研究吉富品系尼罗罗非鱼群体内的形态差异。结果显示,吉富罗非鱼体高与体质量的相关性最大(R2=0.9002)。在体长与体质量对应的点状分布中,总群体、雌雄群体和每个家系群体中均呈现出2条带状,分布于2条带的个体间质量差异不显著(P>0.05),对应的个体数量之比接近常数0.7;体高、体宽、头长、尾柄长和尾柄高相应的分布则呈现出变异幅度迥异的条带。本研究表明,吉富罗非鱼群体内部在形态上存在差异。
文摘用投喂添加色素的膨化颗粒饲料和观测排便相结合的方法,研究吉富品系尼罗罗非鱼(Genetic Improvement of Farmed Tilapias;GIFT)的摄食节律。结果发现,6个家系的吉富品系尼罗罗非鱼摄食具有明显的节律性,家系之间的摄食周期存在非常显著差异(P<0.0001);t检验发现,各家系内摄食周期和排便周期的差异均不显著(P≥0.1097)。回归分析发现,摄食周期对生长无显著的影响(P=0.8988),但与摄食量之间存在显著的线性关系(R2=0.9679,P=0.0004)。对摄食节律聚类分析,6个家系可分为(1、2、6)、(3、4)、5共3个家系类别。该项研究能为GIFT的养殖投饵和进一步选育提供理论依据,为鱼类摄食节律研究提供方法上的参考。
