Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduc...Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.展开更多
为阐明GII.12型诺如病毒与组织血型抗原(Histo-blood group antigens,HBGAs)受体结合模式,本研究首先合成GII.12型诺如病毒毒株Pune株(GenBank登录号:EU921353)P区基因序列,并在原核系统中表达P蛋白,利用快速液相色谱分析鉴定P粒子的形...为阐明GII.12型诺如病毒与组织血型抗原(Histo-blood group antigens,HBGAs)受体结合模式,本研究首先合成GII.12型诺如病毒毒株Pune株(GenBank登录号:EU921353)P区基因序列,并在原核系统中表达P蛋白,利用快速液相色谱分析鉴定P粒子的形成,将P粒子免疫小鼠,应用HBGAs表型明确的唾液样本分析P粒子的HBGAs结合模式。通过唾液结合分析,GII.12型诺如病毒Pune株与B型、AB型唾液结合较高,与A型、O型分泌型及O型非分泌型唾液结合较低,表明GII.12型诺如病毒与B抗原亲和性更高,这与先前GII.12型诺如病毒晶体结构的研究一致。这些研究提示,B型、AB型个体相对于其他血型个体,可能对GII.12型诺如病毒更具易感性。GII.12型诺如病毒与B抗原有更高的亲和性,为GII.12型诺如病毒的预防和控制提供了科学基础。展开更多
基金funded by the Science and Technology Development Fund,Macao SAR(Nos.0065/2020/A2,SKLQRCM(MUST)-2020-2022)Shenzhen-Hong Kong-Macao Science and Technology Project(Grade c)(No.SGDX20210823104201010).
文摘Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.
文摘为阐明GII.12型诺如病毒与组织血型抗原(Histo-blood group antigens,HBGAs)受体结合模式,本研究首先合成GII.12型诺如病毒毒株Pune株(GenBank登录号:EU921353)P区基因序列,并在原核系统中表达P蛋白,利用快速液相色谱分析鉴定P粒子的形成,将P粒子免疫小鼠,应用HBGAs表型明确的唾液样本分析P粒子的HBGAs结合模式。通过唾液结合分析,GII.12型诺如病毒Pune株与B型、AB型唾液结合较高,与A型、O型分泌型及O型非分泌型唾液结合较低,表明GII.12型诺如病毒与B抗原亲和性更高,这与先前GII.12型诺如病毒晶体结构的研究一致。这些研究提示,B型、AB型个体相对于其他血型个体,可能对GII.12型诺如病毒更具易感性。GII.12型诺如病毒与B抗原有更高的亲和性,为GII.12型诺如病毒的预防和控制提供了科学基础。
