背景与目的 肺癌是全球最常见的恶性肿瘤,大多数患者初次诊断时已发生转移,因此寻找肺癌新的诊断标志物并探索其在肺癌发生中的作用具有重要价值。基于生物信息数据探讨分析肺癌中GINS1基因与肺癌预后的相关性具有重要意义。方法 检索On...背景与目的 肺癌是全球最常见的恶性肿瘤,大多数患者初次诊断时已发生转移,因此寻找肺癌新的诊断标志物并探索其在肺癌发生中的作用具有重要价值。基于生物信息数据探讨分析肺癌中GINS1基因与肺癌预后的相关性具有重要意义。方法 检索Oncomine、EGPIA、TCGA、Human protein atlas等基因数据库,分析GINS1基因在肺癌中的表达差异。应用蛋白质免疫印记法检测肺癌及癌旁组织中GINS1蛋白的表达水平,应用Kaplan-Meier进行患者生存分析,并利用String-DB数据库分析GINS1蛋白相互作用网络。结果 对Oncomine、GEPIA和The Human Protein Atlas数据库中肺癌与癌旁组织蛋白表达数据进行差异性分析,发现GINS1蛋白在肺癌组织中显著高表达,特别以肺腺癌、肺鳞癌中表达明显增加;通过生存分析发现高表达GINS1肺腺癌患者生存期明显低于低表达者,高表达患者预后更差;利用String-DB数据库发现与GINS1蛋白关联最为密切的为GINS蛋白家族(GINS2、GINS3、GINS4),其次为CDC45、MCM蛋白家族(MCM2、MCM3、MCM4、MCM5、MCM6、MCM7),富集分析发现互作蛋白主要参与DNA复制和细胞周期。结论GINS1基因在肺癌组织中显著高表达,与患者预后相关,其有可能成为肺癌诊断及药物治疗的新靶点。展开更多
OBJECTIVE： Previous reports have demonstrated that X-ray repair cross-complementing gene 1 （XRCCl） Arg399GIn polymorphism is a possible risk factor for several cancers. Published data on the association of XRCCl Ar...OBJECTIVE： Previous reports have demonstrated that X-ray repair cross-complementing gene 1 （XRCCl） Arg399GIn polymorphism is a possible risk factor for several cancers. Published data on the association of XRCCl Arg399GIn polymorphism with glioma susceptibility have generated conflicting results. This study is designed to precisely estimate the relationship. DATA RETRIEVAL： A computer-based online retrieval of Medline, EMBASE, OVID, Sciencedirect, and Chinese National Knowledge Infrastructure was performed to search papers regarding association of XRCC1 Arg399GIn polymorphisms with glioma published up to April 2012. SELECTION CRITERIA： Two investigators selected data independently. Meta analysis was then performed for the selected studies using STATA 11.0 software after strict selection. Heterogeneity test, sensitivity analysis and publication bias assessments were then conducted. MAIN OUTCOME MEASURES： Association of XRCCl Arg399GIn polymorphism with glioma risk. RESULTS： A total of nine case-controlled studies comprising 2 326 cases and 3 610 controls were selected for final analysis. The overall data failed to indicate a significant association of XRCCl Arg399GIn polymorphism with glioma risk （Gin/Gin vs. Arg/Arg： odds ratio （OR） = 1.11; 95% confidence interval （Cl） = 0.94-1.31; dominant model： OR = 1.06; 95%C/= 0.95-1.18; recessive model： OR = 1.04; 95%C/= 0.81-1.34）. However, subgroup analysis regarding ethnicity showed an increased risk among Asians （Gin/Gin vs. Arg/Arg OR = 1.40; 95%C/= 1.10-1.78; recessive model： Caucasians or mixed ethnicities. OR = 1.70; 95%Cl = 1.17-2.46; dominant mode OR = 1.46; 95%C/= 1.04-2.05） but not CONCLUSION： XRCCl Arg399GIn polymorphism might modify the susceptibility to glioma among Asians but not Caucasians. Further large and well-designed studies are needed to confirm this conclusion.展开更多
文摘背景与目的 肺癌是全球最常见的恶性肿瘤,大多数患者初次诊断时已发生转移,因此寻找肺癌新的诊断标志物并探索其在肺癌发生中的作用具有重要价值。基于生物信息数据探讨分析肺癌中GINS1基因与肺癌预后的相关性具有重要意义。方法 检索Oncomine、EGPIA、TCGA、Human protein atlas等基因数据库,分析GINS1基因在肺癌中的表达差异。应用蛋白质免疫印记法检测肺癌及癌旁组织中GINS1蛋白的表达水平,应用Kaplan-Meier进行患者生存分析,并利用String-DB数据库分析GINS1蛋白相互作用网络。结果 对Oncomine、GEPIA和The Human Protein Atlas数据库中肺癌与癌旁组织蛋白表达数据进行差异性分析,发现GINS1蛋白在肺癌组织中显著高表达,特别以肺腺癌、肺鳞癌中表达明显增加;通过生存分析发现高表达GINS1肺腺癌患者生存期明显低于低表达者,高表达患者预后更差;利用String-DB数据库发现与GINS1蛋白关联最为密切的为GINS蛋白家族(GINS2、GINS3、GINS4),其次为CDC45、MCM蛋白家族(MCM2、MCM3、MCM4、MCM5、MCM6、MCM7),富集分析发现互作蛋白主要参与DNA复制和细胞周期。结论GINS1基因在肺癌组织中显著高表达,与患者预后相关,其有可能成为肺癌诊断及药物治疗的新靶点。
文摘目的 筛选卵巢癌预后不良的分子生物学标志。方法 从GEO数据库获得卵巢癌GSE14001、GSE14407数据集,用在线分析工具GEO2R、Venn筛选得到卵巢癌和正常卵巢组织差异表达基因(DEGs),对DEGs进行富集分析,构建蛋白互作网络(PPI),以及构建网络模块得到关键基因,利用Kaplan Meier plotter网站分析关键基因与卵巢癌患者总生存期之间关系,应用GEPIA数据库分析DEGs在卵巢和卵巢癌组织中表达,应用The Human Protein Atlas数据库获取筛选基因的免疫组化结果,对比它们在卵巢和卵巢癌组织间的表达差异。结果 得到211个DEGs,92个上调和119个下调。差异基因生物学过程主要涉及侧枝发芽的正向调控、乏氧反应、间充质-上皮细胞信号传导;细胞组分主要集中在质膜顶、细胞表面、细胞外外泌体等部位;分子功能主要涉及丝氨酸型内肽酶活性、金属内肽酶活性、受体活性等;信号通路富集于白细胞跨内皮细胞迁移、细胞黏附通路、癌症通路等信号通路。得到35个候选基因,其中14个基因高表达、8个基因低表达与患者总生存期相关(P<0.05)。其中ATP6V1F、GINS1、GINS4基因在卵巢癌组织中在RNA水平和蛋白质水平表达均高于正常组织(P<0.05)。GNB3在卵巢癌组织中RNA水平表达低于正常组织,而在蛋白质水平恰好相反(P<0.05)。结论 ATP6V1F、GINS1、GINS4有可能是卵巢癌预后不良的新分子标志物。
文摘OBJECTIVE： Previous reports have demonstrated that X-ray repair cross-complementing gene 1 （XRCCl） Arg399GIn polymorphism is a possible risk factor for several cancers. Published data on the association of XRCCl Arg399GIn polymorphism with glioma susceptibility have generated conflicting results. This study is designed to precisely estimate the relationship. DATA RETRIEVAL： A computer-based online retrieval of Medline, EMBASE, OVID, Sciencedirect, and Chinese National Knowledge Infrastructure was performed to search papers regarding association of XRCC1 Arg399GIn polymorphisms with glioma published up to April 2012. SELECTION CRITERIA： Two investigators selected data independently. Meta analysis was then performed for the selected studies using STATA 11.0 software after strict selection. Heterogeneity test, sensitivity analysis and publication bias assessments were then conducted. MAIN OUTCOME MEASURES： Association of XRCCl Arg399GIn polymorphism with glioma risk. RESULTS： A total of nine case-controlled studies comprising 2 326 cases and 3 610 controls were selected for final analysis. The overall data failed to indicate a significant association of XRCCl Arg399GIn polymorphism with glioma risk （Gin/Gin vs. Arg/Arg： odds ratio （OR） = 1.11; 95% confidence interval （Cl） = 0.94-1.31; dominant model： OR = 1.06; 95%C/= 0.95-1.18; recessive model： OR = 1.04; 95%C/= 0.81-1.34）. However, subgroup analysis regarding ethnicity showed an increased risk among Asians （Gin/Gin vs. Arg/Arg OR = 1.40; 95%C/= 1.10-1.78; recessive model： Caucasians or mixed ethnicities. OR = 1.70; 95%Cl = 1.17-2.46; dominant mode OR = 1.46; 95%C/= 1.04-2.05） but not CONCLUSION： XRCCl Arg399GIn polymorphism might modify the susceptibility to glioma among Asians but not Caucasians. Further large and well-designed studies are needed to confirm this conclusion.
