目的探讨GLI-2(GLI family zinc finger 2)和SFRP-1(Secreled frizzled-related protein 1)在髓母细胞瘤(Medulloblastoma,MB)组织中的表达及其意义。方法采用SP免疫组化法检测32份MB组织中GLI-2和SFRP-1的表达,分析两种蛋白之间及其与M...目的探讨GLI-2(GLI family zinc finger 2)和SFRP-1(Secreled frizzled-related protein 1)在髓母细胞瘤(Medulloblastoma,MB)组织中的表达及其意义。方法采用SP免疫组化法检测32份MB组织中GLI-2和SFRP-1的表达,分析两种蛋白之间及其与MB临床病理特征之间的相关性。结果 32例MB中,GLI-2和SFRP-1的阳性表达率分别为50%(16/32)和68.75%(22/32);MB中GLI-2和SFRP-1的表达在不同年龄、性别、肿瘤大小及部位中,差异均无统计学意义(P>0.05);SFRP-1在SHH-MB与NON-SHH-MB中的表达差异有统计学意义(P<0.001);GLI-2与SFRP-1在MB中的表达呈显著正相关(P<0.001)。结论 GLI-2和SFRP-1的异常表达可能对MB的发生、发展起一定的作用。GLI-2和SFRP-1可作为MB重要的生物学指标,有助于对MB患者危险度评估及患者术后放化疗方案的选择。展开更多
Objective: To investigate the changes and mechanism of angiogenesis in myocardium induced by transplantation of the sonic hedgehog (shh) gene transfected in bone marrow mesenchymal stem cells (BMMSC) after myocardial ...Objective: To investigate the changes and mechanism of angiogenesis in myocardium induced by transplantation of the sonic hedgehog (shh) gene transfected in bone marrow mesenchymal stem cells (BMMSC) after myocardial infarction. Methods: A rat model of acute myocardial infarction was made by coronary artery ligation. The rats were randomly divided into five groups of 40 rats each. These were further subdivided into groups of 10 rats. The peripheral regions of the infarcts were injected with either BMMSCSHH (transfection group), equivalent BMMSC (cell only group), BMMSC and pcDNA3.1-Shh DNA mixture (mixture group), pcDNA3.1-shh DNA alone (gene only group), or equal volumes of low-sugar DMEM medium (control group). One, two, four, and eight weeks after transplantation, specimens were harvested from the transplantation site to determine the expression of SHH signaling pathway downstream genes Ptc1, Gli-2, COUP-TF II, angiogenesis promoting factor VEGF, and Ang-1 using RT-PCR. Results: Seven days after transplantation, the expression of SHH signaling pathway downstream genes, Ptc1, Gli-2, and COUP-TF II was significantly more pronounced in the transfection group than in the control group, cell only group, gene only group, or mixture group (Ptc1: P P P < 0.05, and P COUP-TF II: P P P P Gli-2: P P P < 0.05, and P < 0.05, respectively). The expression of angiogenesis-promoting genes Vegf and Ang-1 was significantly more pronounced than in the control group, cell only group, or gene only group (Vegf: P P P Ang-1: P P P < 0.05, respectively). Conclusion: Transplantation of the shh-gene-transfected BMMSCs to the peripheral regions of myocardial infarcts promoted angiogenesis by upregulating downstream gene expression.展开更多
文摘目的探讨GLI-2(GLI family zinc finger 2)和SFRP-1(Secreled frizzled-related protein 1)在髓母细胞瘤(Medulloblastoma,MB)组织中的表达及其意义。方法采用SP免疫组化法检测32份MB组织中GLI-2和SFRP-1的表达,分析两种蛋白之间及其与MB临床病理特征之间的相关性。结果 32例MB中,GLI-2和SFRP-1的阳性表达率分别为50%(16/32)和68.75%(22/32);MB中GLI-2和SFRP-1的表达在不同年龄、性别、肿瘤大小及部位中,差异均无统计学意义(P>0.05);SFRP-1在SHH-MB与NON-SHH-MB中的表达差异有统计学意义(P<0.001);GLI-2与SFRP-1在MB中的表达呈显著正相关(P<0.001)。结论 GLI-2和SFRP-1的异常表达可能对MB的发生、发展起一定的作用。GLI-2和SFRP-1可作为MB重要的生物学指标,有助于对MB患者危险度评估及患者术后放化疗方案的选择。
文摘Objective: To investigate the changes and mechanism of angiogenesis in myocardium induced by transplantation of the sonic hedgehog (shh) gene transfected in bone marrow mesenchymal stem cells (BMMSC) after myocardial infarction. Methods: A rat model of acute myocardial infarction was made by coronary artery ligation. The rats were randomly divided into five groups of 40 rats each. These were further subdivided into groups of 10 rats. The peripheral regions of the infarcts were injected with either BMMSCSHH (transfection group), equivalent BMMSC (cell only group), BMMSC and pcDNA3.1-Shh DNA mixture (mixture group), pcDNA3.1-shh DNA alone (gene only group), or equal volumes of low-sugar DMEM medium (control group). One, two, four, and eight weeks after transplantation, specimens were harvested from the transplantation site to determine the expression of SHH signaling pathway downstream genes Ptc1, Gli-2, COUP-TF II, angiogenesis promoting factor VEGF, and Ang-1 using RT-PCR. Results: Seven days after transplantation, the expression of SHH signaling pathway downstream genes, Ptc1, Gli-2, and COUP-TF II was significantly more pronounced in the transfection group than in the control group, cell only group, gene only group, or mixture group (Ptc1: P P P < 0.05, and P COUP-TF II: P P P P Gli-2: P P P < 0.05, and P < 0.05, respectively). The expression of angiogenesis-promoting genes Vegf and Ang-1 was significantly more pronounced than in the control group, cell only group, or gene only group (Vegf: P P P Ang-1: P P P < 0.05, respectively). Conclusion: Transplantation of the shh-gene-transfected BMMSCs to the peripheral regions of myocardial infarcts promoted angiogenesis by upregulating downstream gene expression.
