2-[3,5-Di-O-β-D-glucosyl-4-(3-methylbut-2-enyl)phenyl]benzofuran-6-ol,a new prenylated arylbenzofuran derivative was isolated from Morus alba L.Its structure was elucidated by various spectroscopic methods including ...2-[3,5-Di-O-β-D-glucosyl-4-(3-methylbut-2-enyl)phenyl]benzofuran-6-ol,a new prenylated arylbenzofuran derivative was isolated from Morus alba L.Its structure was elucidated by various spectroscopic methods including MS,~1H NMR,^(13)C NMR,DEPT,~1H-~1HCOSY,HMQC and HMBC.展开更多
Thermodynamical data of rare earth complexes with amino acid are important for engineering chemistry and fundamental chemistry. However, they have rarely been reported. In this work, a series of crystalline complexes ...Thermodynamical data of rare earth complexes with amino acid are important for engineering chemistry and fundamental chemistry. However, they have rarely been reported. In this work, a series of crystalline complexes of rare earth perchlorate coordinated with glutamic acid have been synthesized in water medium, and their thermodynamical data, including the heat capacity in low temperature range and the standard enthalpy of formation, were determined. These complexes were identified to be [RE2(Glu)2(H2O)8](ClO4)4·H2O (RE = Nd, Eu, Dy) by using thermogravimetric analysis (TG), differential thermal analysis (DTA), and chemical and elementary analyses. Their purity has been determined. No melting points were observed for all the three complexes. The heat capacity of the complexes was measured by an adiabatic calorimeter from 79 to 370 K. Abnormal heat capacity values were detected for two of the complexes and the decomposition range of one complex was found. The temperature, enthalpy change and entropy change of the decomposition processes of the three complexes were calculated. The polynomial equations of heat capacity in the experimental temperature range have been obtained by least squares fitting. The standard enthalpy of formation was determined by an isoperibol reaction calorimeter at 298.15 K.展开更多
A coordination compound of erbium perchlorate with L-α-glutamic acid, [Er2（Glu）2（H2O）6]（ClO4）4·6H2O（s）, was synthesized. By chemical analysis, elemental analysis, FTIR, TG/DTG, and comparison with releva...A coordination compound of erbium perchlorate with L-α-glutamic acid, [Er2（Glu）2（H2O）6]（ClO4）4·6H2O（s）, was synthesized. By chemical analysis, elemental analysis, FTIR, TG/DTG, and comparison with relevant literatures, its chemical composition and structure were established. The mechanism of thermal decomposition of the complex was deduced on the basis of the TG/DTG analysis. Low-temperature heat capacities were measured by a precision automated adiabatic calorimeter from 78 to 318 K. An endothermic peak in the heat capacity curve was observed over the temperature region of 290-318 K, which was ascribed to a solid-to-solid phase transition. The temperature Ttrans, the enthalpy △transHm and the entropy △transSm of the phase transition for the compound were determined to be： （308.73±0.45） K, （10.49±0.05） kJ·mol^-1 and （33.9±0.2） J·K^-1·mol^-1. Polynomial equation of heat capacities as a function of the temperature in the region of 78-290 K was fitted by the least square method. Standard molar enthalpies of dissolution of the mixture [2ErCl3·6H2O（s）＋2L-Glu（s）＋6NaClO4·H2O（s）] and the mixture {[Er2（Glu）2（H2O）6]（ClO4）4·6H2O（s）＋6NaCl（s）} in 100 mL of 2 mol·dm^-3 HClO4 as calorimetric solvent, and {2HClO4（1）} in the solution A＇ at T=298.15 K were measured to be, △dHm,1=（31.552±0.026） kJ·mol^-1, △dHm,2 = （41.302±0.034） kJ·mol^-1, and △dHm,3 = （ 14.986 ± 0.064） kJ·mol^-1, respectively. In accordance with Hess law, the standard molar enthalpy of formation of the complex was determined as △fHm-=-（7551.0±2.4） kJ·mol^-1 by using an isoperibol solution-reaction calorimeter and designing a thermochemical cycle.展开更多
目的在妊娠期糖尿病(GDM)中探讨miR-372-3p表达及其与胰岛素抵抗(IR)的相互关系并初步探究其分子机制;方法选取2018年1月至2019年1月于兰州大学第二附属医院正规产检并住院分娩的56例GDM孕妇为GDM组,选取同期在本院分娩的60例正常孕妇...目的在妊娠期糖尿病(GDM)中探讨miR-372-3p表达及其与胰岛素抵抗(IR)的相互关系并初步探究其分子机制;方法选取2018年1月至2019年1月于兰州大学第二附属医院正规产检并住院分娩的56例GDM孕妇为GDM组,选取同期在本院分娩的60例正常孕妇作为正常对照组(NC组)。观察对比两组孕妇一般临床资料(如年龄、产前BMI、OGTT孕周等)和代谢相关生化指标(总胆固醇(TC)、三酰甘油(TG)空腹血糖值(FPG)、餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)等),并计算胰岛β细胞功能指数(HOMA-β)及胰岛素抵抗指数(HOMA-IR);RT-PCR检测两组受试者胎盘组织中的miR-372-3p的表达水平;Pearson相关系数分析GDM组中miR-372-3p表达量与代谢相关指标的相关性;生物信息学选取GLUT-4作为miR-372-3p的目标靶基因,并采用双荧光素酶基因报告实验进行验证两者的靶向关系;在HTR-8细胞中转染miR-372-3p mimics或miR-372-3p inhibitor进行上调或抑制miR-372-3p表达,RT-PCR检测转染效率后,Western blot实验检测miR-372-3p对GLUT-4蛋白表达的影响。结果与NC组相比,GDM组孕产妇仅产前BMI显著增加(P<0.01),其余临床资料差异均无统计学意义(P>0.05);代谢相关指标相比,GDM组的TC、TG、FPG、2 h PG、FINS及HOMA-IR均较NC组显著增加(P<0.01),而HOMA-β显著低于NC组(P<0.01),其余指标差异均无统计学意义(P>0.05);GDM组的胎盘miR-372-3p表达较NC组显著升高(P<0.05)且其表达水平与FPG、FINS、HOMA-IR均呈正相关关系(P<0.05);双荧光素酶基因报告实验证实miR-372-3p可靶向调控GLUT-4表达且在HTR-8细胞中miR-372-3p可负向调控GLU-4蛋白表达;结论在GDM中miR-372-3p可能通过负向调控GLUT-4表达参与胰岛素抵抗。展开更多
OBJECTIVE: To investigate the antiepileptic effects of Chaihushugan decoction(CHSGD) in rats with pentylenetetrazole(PTZ)-induced seizures and to discuss the impact of CHSGD on glutamate metabolism, a hypothesized und...OBJECTIVE: To investigate the antiepileptic effects of Chaihushugan decoction(CHSGD) in rats with pentylenetetrazole(PTZ)-induced seizures and to discuss the impact of CHSGD on glutamate metabolism, a hypothesized underlying mechanism of seizure reduction.METHODS: Fifty Wistar rats were divided randomly into either control(n = 10) or experimental(n = 40)groups. Rats in the control group were administered physiological saline intraperitoneally. A subconvulsive dose of PTZ(35 mg/kg) was administered intraperitoneally to rats in the experimental group to induce seizures. The fully PTZ-kindled rats were then randomly divided into five subgroups(n = 8 each) based on the following treatment categories: physiological saline, VPA(200 mg/kg), CHSGD(2.5 g/kg), CHSGD(5 g/kg), or CHSGD(10 g/kg),administered orally once per day, respectively. On day 28 following initiation of drug treatment, seizures were monitored. The rats were then sacrificed, and hippocampal dissections were performed for subsequent studies.RESULTS: CHSGD significantly prolonged the latency of myoclonic, clonic, and tonic seizures, while decreasing overall seizure rates in the kindled rats.The measured concentrations of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose(2-NBDG) and glutamate were significantly lower in the hippocampi of kindled rats in groups treated with CHSGD compared with those treated with PTZ alone. In addition, CHSGD was found to up-regulate both the expression of glutamate transporter-1(GLT-1) protein and the activity of glutamine synthetase(GS) in the hippocampi of kindled rats.CONCLUSION: These results suggest that CHSGD has antiepileptic effects on PTZ-induced seizures.The results further suggest an increase in glutamate metabolism at the synaptic cleft is a putative underlying mechanism of seizure reduction.展开更多
基金supported by the Great Research Project of National Major New Drug Development(No. 2009ZX09102-110)
文摘2-[3,5-Di-O-β-D-glucosyl-4-(3-methylbut-2-enyl)phenyl]benzofuran-6-ol,a new prenylated arylbenzofuran derivative was isolated from Morus alba L.Its structure was elucidated by various spectroscopic methods including MS,~1H NMR,^(13)C NMR,DEPT,~1H-~1HCOSY,HMQC and HMBC.
基金Supported by the Research Fund of Beijing Institute of Petro-Chemical Technology (N06-06)
文摘Thermodynamical data of rare earth complexes with amino acid are important for engineering chemistry and fundamental chemistry. However, they have rarely been reported. In this work, a series of crystalline complexes of rare earth perchlorate coordinated with glutamic acid have been synthesized in water medium, and their thermodynamical data, including the heat capacity in low temperature range and the standard enthalpy of formation, were determined. These complexes were identified to be [RE2(Glu)2(H2O)8](ClO4)4·H2O (RE = Nd, Eu, Dy) by using thermogravimetric analysis (TG), differential thermal analysis (DTA), and chemical and elementary analyses. Their purity has been determined. No melting points were observed for all the three complexes. The heat capacity of the complexes was measured by an adiabatic calorimeter from 79 to 370 K. Abnormal heat capacity values were detected for two of the complexes and the decomposition range of one complex was found. The temperature, enthalpy change and entropy change of the decomposition processes of the three complexes were calculated. The polynomial equations of heat capacity in the experimental temperature range have been obtained by least squares fitting. The standard enthalpy of formation was determined by an isoperibol reaction calorimeter at 298.15 K.
基金Project supported by the National Natural Science Foundation of China （No. 20673050）.
文摘A coordination compound of erbium perchlorate with L-α-glutamic acid, [Er2（Glu）2（H2O）6]（ClO4）4·6H2O（s）, was synthesized. By chemical analysis, elemental analysis, FTIR, TG/DTG, and comparison with relevant literatures, its chemical composition and structure were established. The mechanism of thermal decomposition of the complex was deduced on the basis of the TG/DTG analysis. Low-temperature heat capacities were measured by a precision automated adiabatic calorimeter from 78 to 318 K. An endothermic peak in the heat capacity curve was observed over the temperature region of 290-318 K, which was ascribed to a solid-to-solid phase transition. The temperature Ttrans, the enthalpy △transHm and the entropy △transSm of the phase transition for the compound were determined to be： （308.73±0.45） K, （10.49±0.05） kJ·mol^-1 and （33.9±0.2） J·K^-1·mol^-1. Polynomial equation of heat capacities as a function of the temperature in the region of 78-290 K was fitted by the least square method. Standard molar enthalpies of dissolution of the mixture [2ErCl3·6H2O（s）＋2L-Glu（s）＋6NaClO4·H2O（s）] and the mixture {[Er2（Glu）2（H2O）6]（ClO4）4·6H2O（s）＋6NaCl（s）} in 100 mL of 2 mol·dm^-3 HClO4 as calorimetric solvent, and {2HClO4（1）} in the solution A＇ at T=298.15 K were measured to be, △dHm,1=（31.552±0.026） kJ·mol^-1, △dHm,2 = （41.302±0.034） kJ·mol^-1, and △dHm,3 = （ 14.986 ± 0.064） kJ·mol^-1, respectively. In accordance with Hess law, the standard molar enthalpy of formation of the complex was determined as △fHm-=-（7551.0±2.4） kJ·mol^-1 by using an isoperibol solution-reaction calorimeter and designing a thermochemical cycle.
文摘目的在妊娠期糖尿病(GDM)中探讨miR-372-3p表达及其与胰岛素抵抗(IR)的相互关系并初步探究其分子机制;方法选取2018年1月至2019年1月于兰州大学第二附属医院正规产检并住院分娩的56例GDM孕妇为GDM组,选取同期在本院分娩的60例正常孕妇作为正常对照组(NC组)。观察对比两组孕妇一般临床资料(如年龄、产前BMI、OGTT孕周等)和代谢相关生化指标(总胆固醇(TC)、三酰甘油(TG)空腹血糖值(FPG)、餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)等),并计算胰岛β细胞功能指数(HOMA-β)及胰岛素抵抗指数(HOMA-IR);RT-PCR检测两组受试者胎盘组织中的miR-372-3p的表达水平;Pearson相关系数分析GDM组中miR-372-3p表达量与代谢相关指标的相关性;生物信息学选取GLUT-4作为miR-372-3p的目标靶基因,并采用双荧光素酶基因报告实验进行验证两者的靶向关系;在HTR-8细胞中转染miR-372-3p mimics或miR-372-3p inhibitor进行上调或抑制miR-372-3p表达,RT-PCR检测转染效率后,Western blot实验检测miR-372-3p对GLUT-4蛋白表达的影响。结果与NC组相比,GDM组孕产妇仅产前BMI显著增加(P<0.01),其余临床资料差异均无统计学意义(P>0.05);代谢相关指标相比,GDM组的TC、TG、FPG、2 h PG、FINS及HOMA-IR均较NC组显著增加(P<0.01),而HOMA-β显著低于NC组(P<0.01),其余指标差异均无统计学意义(P>0.05);GDM组的胎盘miR-372-3p表达较NC组显著升高(P<0.05)且其表达水平与FPG、FINS、HOMA-IR均呈正相关关系(P<0.05);双荧光素酶基因报告实验证实miR-372-3p可靶向调控GLUT-4表达且在HTR-8细胞中miR-372-3p可负向调控GLU-4蛋白表达;结论在GDM中miR-372-3p可能通过负向调控GLUT-4表达参与胰岛素抵抗。
基金Supported by Guangdong Natural Science Foundation(The effects of "Treatment from Gan"on Regulation of A-type Potassium Channels by KChIP/Kv4 in the pathomechanism of Refractory Epilepsy,No.2014A030310052)National Natural Science Foundation of China(Study on Regulation of A-type Potassium Channels by KChIP/Kv4 in the Pathomechanism of Refractory Epilepsy and the Effects of "Treatment from Gan",No.81503564)
文摘OBJECTIVE: To investigate the antiepileptic effects of Chaihushugan decoction(CHSGD) in rats with pentylenetetrazole(PTZ)-induced seizures and to discuss the impact of CHSGD on glutamate metabolism, a hypothesized underlying mechanism of seizure reduction.METHODS: Fifty Wistar rats were divided randomly into either control(n = 10) or experimental(n = 40)groups. Rats in the control group were administered physiological saline intraperitoneally. A subconvulsive dose of PTZ(35 mg/kg) was administered intraperitoneally to rats in the experimental group to induce seizures. The fully PTZ-kindled rats were then randomly divided into five subgroups(n = 8 each) based on the following treatment categories: physiological saline, VPA(200 mg/kg), CHSGD(2.5 g/kg), CHSGD(5 g/kg), or CHSGD(10 g/kg),administered orally once per day, respectively. On day 28 following initiation of drug treatment, seizures were monitored. The rats were then sacrificed, and hippocampal dissections were performed for subsequent studies.RESULTS: CHSGD significantly prolonged the latency of myoclonic, clonic, and tonic seizures, while decreasing overall seizure rates in the kindled rats.The measured concentrations of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose(2-NBDG) and glutamate were significantly lower in the hippocampi of kindled rats in groups treated with CHSGD compared with those treated with PTZ alone. In addition, CHSGD was found to up-regulate both the expression of glutamate transporter-1(GLT-1) protein and the activity of glutamine synthetase(GS) in the hippocampi of kindled rats.CONCLUSION: These results suggest that CHSGD has antiepileptic effects on PTZ-induced seizures.The results further suggest an increase in glutamate metabolism at the synaptic cleft is a putative underlying mechanism of seizure reduction.
