STUDY ON C. D. SPECTRA OF SUBSTITUTED-C-PHENYL-D-GLUCOPYRANOSIDES

Ⅰ. INTRODUCTION C. D. curves can be applied to determination of the configuration of sugar and glycosides. Our study reports the C.D. data of several substituted-C-phenyl glucopyranosides, synthesized by the previously described method. The crude product of Cglycosides was submitted to silica gel column chromatography or preparative thin layer chromatography to separate the anomers, whose configuration was determined by ~1HNMR.

Phenolic glucopyranosides from the Chinese mangrove plant Excoecaria agallocha L.

Chemical examination of a Chinese mangrove plant Excoecaria agallocha L.led to the isolations of a new glucoside named l-（3,5-dimethoxy-4-hydroxybenzyl）-6-O-galloyl-l-O-β-D-glucopyranoside（1）,together with 10 known glycosides including 3,5-dimethoxy-4-hydroxybenzyl-l-O-β-D-glucopyranoside（2）,hydrangeifolin I（3）,（2-methoxy-5-hydroxymethyl-phenyl） -l-O-β-D-（6-Ogalloyl） glucopyranoside（4）,（3,5-dimethoxy-4-hydroxyphenyl）-l-O-β-D-（6-O-galloyl） glucopyranoside （5）,3-methoxy-4-hydroxyphenyl l-O-β-D-（6-O-galloyl） glucopyranoside（6）,3,4,5-trimethoxyphenyl-（6-O-galloyl）-O-β-D-glucopyranoside （7）,koaburaside（8）,koaburaside monomethyl ether（9）,cuneatasides D（10）,and（？）-isosyringin（11）.Their structures were determined through the extensive spectroscopic analysis and comparison with the reported data in literature. Compounds 2-6,8-11 were isolated from the genus Excoecaria for the first time.

New coumarin glucopyranosides from roots of Angelica dahurica

Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.

无梗五加果化学成分的研究(英文)

从无梗五加(Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem.)果70%乙醇提取物中分离鉴定了12个化合物。经波谱鉴定为东莨菪内酯(1),原儿茶酸甲酯(2),槲皮素(3),无梗五加苷B(4),金丝桃苷(5),(-)-pinoresinol-4,4′-di-O-β-D-glucopyranoside(6),chiisanoside(7),22α-hydroxychiisanoside(8),niduloic acid(9),胡萝卜苷(10),东莨菪苷(11)和无梗五加苷D(12)。其中化合物9为新天然产物,化合物1,2,6和11是首次从五加科植物中分离得到,化合物3是首次从五加属植物中分离得到。

竹叶兰的化学成分研究

目的:研究竹叶兰的化学成分。方法:利用各种色谱技术进行分离纯化,根据各种波谱数据和文献数据对照进行结构鉴定。结果:分离鉴定两个化合物,分别为化合物3’,3’-二羟基-5’-甲氧基联苄(batatasinⅢ)(Ⅰ)、山奈酚-3-0-β-D-葡萄糖苷(kaempferol-3-0-β-D-glucopyranoside)(Ⅱ),化合物。结论:两个化合物均为首次从竹叶兰中分离得到。

Two new glycosides from the seeds of Cassia obtusifolia

Two new glycosides, nor-rubrofusadn-6-O-β-D-（6＇-O-acetyl）glucopyranoside （1） and 1-desmethylaurantio-obtusin-2-O-β-D- glucopyranoside （2） were isolated from the seeds of Cassia obtusifolia and their structures were established by chemical and spectral evidences. 2009 Yong Qing Xiao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats

Delonix elata L.is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic problems.However,systematic evaluation of its wound healing activity is lacking.Thus,in the present study,we aimed to assess the wound healing activity of D.elata stem bark extract(DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside(QRPG) in rats.The formulations effects on wound healing were assessed by the wound contraction rate,epithelialization period,tensile strength,content of the hydroxyproline,hexosamine and uronic acid in granulation tissue,histopathological studies and Col 1 α(I) expression level in wound tissue by reverse transcription polymerase chain reaction(RT-PCR) study.The topical application of DSE ointment caused faster epithelialization,significant wound contraction(100%),and better tensile strength(710.5 ± 10.5 g/cm^2),while QRPG showed wound epithelialization with 98.2%contraction,better than that of the control group(78.18%).The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline,hexosamine and uronic acid content.A significant increase in the expression of Col 1 α(I) was observed in the wound tissue of DSE and QRPG treated rats.DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulation of Col 1 α(I),thus validating ethnomedicinal uses.

A New Flavonol Glycoside from Anaphalis sinica Hance

A new flavonol glycoside, 5,7,3,4 tetrahydroxy 3 methoxyflavonol 3 O β D glucopyranoside(1) was isolated along with quercetin(2), quercetin 3 O α L rhamnopyranoside(3), quercetin 3 O β D glucopyranoside(4) and kaempferol 3 O β D (6' O p coumaroyl) glucopyranoside(5) from Anaphalis sinica Hance. Their structures were determined on the basis of spectral data(MS, UV, IR, 1H and 13 C NMR). The structure of compound 1 was confirmed by X ray crystallographic analysis.

Two New Sterols from Bolbostemma paniculatum

Two new sterols were isolated from bulbs of Bolbostemma paniculatum (Maxim.) Franquet. Their structures were elucidated as stigmasta-7, 22, 25-triene-3-O-nonadecanoic acid ester (1) and stigmasta-7, 22, 25-triene-3-O-B-D- (6'-palmitoyl) glucopyranoside (2) by chemical and spectroscopic methods.

Bioguided Fractionation and Purification of Natural Bioactives Obtained from<i>Alpinia conchigera</i>Water Extract with Melanin Inhibition Activity

Crude extracts of Alpinia conchigera a species from the Malaysian Ginger (Zingiberaceae) family and its fractions obtained from various extraction methods were assayed for melanogenesis inhibition activity and cell viability. The crude extract obtained from the ethanolic extraction and the super critical fluid extraction did not exhibit significant melanin inhibition activity and was shown to be toxic to the melanocyte cells in comparison to the water extract. The crude aqueous extracts displayed melanin inhibition of 96.38 ± 1.60% and cell viability 109.90 ± 8.32% at a concentration of 500 μg/mL. Bioassay guided fractionation was performed on the water extracts to isolate the active compounds. The actives were identified as trans-cinnamaldehyde and chavicol glucopyranoside with both compound showing potent anti-melanogenesis activity. At 4.9 μg/ml, both trans-cinnamaldehyde and chavicol glucopyranoside gave 85% inhibition of melanin formation in vitro with 77% and 97% cell viability respectively. In comparison, kojic acid, a known skin lightening agent showed 90.0% inhibition at 100 μg/mL. The bioactive composition comprising the extract, active fraction, purified compounds or mixture thereof of Alpinia conchigera may be used for cosmetic and pharmaceutical applications, particularly for the purposes of reducing skin pigmentation.

红花水提取物的化学成分研究

目的对红花水提取物中的化学成分进行研究。方法通过大孔吸附树脂、Sephadex LH-20、反相HPLC等多种色谱进行分离纯化，根据理化性质和波谱数据进行鉴定。结果从红花水提物中分离得到了12个化合物，分别为（2E，8E）-12R—tetradeeadiene-4，6-diyne-1，12，14-triol（1），（8Z）-decaene-4，6-diyn-1—0-B-D—glucopyranoside（2），（8E）-decaene-4，6-diyn-1-0胡-D—glucopyranoside（3），（2S）-4’，5，6，7-四羟基二氢黄酮6-01B—D-吡喃葡萄糖苷（4），对羟基苯甲酸（5），异香草酸（6），dihydropha—seicacidmethylester．3-D移-D—glucoside（7），dihydrophaseicacidmethylester（8），对羟基苯甲醛（9），N，N＇-dicoumaroyl-putrescine（10），4-（4＇-羟基苯基）-丁-3-烯-2-酮（11），对羟基苯乙酮（12）。结论化合物1，3～4，6，8，10为首次从红花中分离得到，并首次报道化合物1的绝对构型及碳谱数据。

HPLC同时测定决明子中4种主要成分的含量

目的：建立HPLC同时测定决明子药材中蒽醌类和萘并吡喃酮类4种主要成分含量的方法,为决明子药材的质量控制提供依据。方法：采用Kromasil 100-5 C18色谱柱（4.6 mm×250 mm,5μm）,流动相水-乙腈-四氢呋喃-冰醋酸（100∶23∶5∶1）等度洗脱。检测波长278 nm,柱温30℃,流速1.0 mL.min-1。结果：4种成分达到了基线分离。红链霉素龙胆二糖苷,决明子苷,aurantio-obtusin-6-O-β-D-glucopyranoside以及决明子苷B线性范围为0.2741.37,0.1530.765,0.3021.51,0.0520.26μg,相关系数r均为0.999 9。平均加样回收率分别为100.9%（RSD 1.8%）,101.2%（RSD 1.2%）,99.40%（RSD 2.2%）,100.5%（RSD 1.6%）。结论：建立了高效液相测定决明子中4种主要成分含量的方法,该方法精确,简便,可靠,重现性好,可用于决明子药材的质量控制。

披针叶茴香果实的化学成分研究

目的研究八角科（Illiciaceae）植物披针叶茴香（Illicium lanceolatum）果实的化学成分。方法应用硅胶、Sephadex LH-20、MCI等各种色谱技术进行分离纯化，根据化合物的理化常数和波谱数据鉴定其结构。结果从披针叶茴香果实的体积分数95％乙醇提取物中分离得到12个化合物，通过渡谱数据和理化常数分别鉴定为2，4-dihydroxy—allylbenzene-2-O-β-D—glucopyranoside（1），（＋）-儿茶素（2），槲皮素-3-O-β-L-鼠李糖苷（3），芦丁（4），异鼠李素-3-D-β-D-芸香糖苷（5），6-deoxy-1-hy—droxy neoanisatin（6），莽草毒素（7），icarisideE3（8），（75，8R）-urolignoside（9），对羟基苯甲酸（10），莽草酸（11），β-谷甾醇（12）。结论化合物1，4～6，8～10为首次从该植物中分离得到。