AIM To assess the direct effects of ischemia onintestinal epithelial integrity. Furthermore, clinicalefforts at mitigating the effect of hypoperfusion on gutpermeability have focused on restoring gut vascularfunction....AIM To assess the direct effects of ischemia onintestinal epithelial integrity. Furthermore, clinicalefforts at mitigating the effect of hypoperfusion on gutpermeability have focused on restoring gut vascularfunction.METHODS： we report that, in the Caco-2 cell modelof transepithelial transport, calcium glycerophosphate（CGP）, an inhibitor of intestinal alkaline phosphataseF3, has a significant effect to preserve transepithelialelectrical resistance （TEER） and to attenuate increasesin mannitol flux rates during hypoxia or cytokinestimulation.RESULTS： The effect was observable even at concentrationsas low as 1 μmol/L. As celiac disease is alsomarked by a loss of gut epithelial integrity, the effectof CGP to attenuate the effect of the α-gliadin peptide31-55 was also examined. In this instance, CGP exertedlittle effect of preservation of TEER, but significantlyattenuated peptide induced increase in mannitol flux.CONCLUSION： it appears that CGP treatment mightsynergize with other therapies to preserve gut epithelialintegrity.展开更多
Chitosan/α,β-glycerophosphate （CS/α,β-GP） thermo-sensitive hydrogels presented flowable solution state at low temperature and semisolid hydrogel when the ambient temperature increased. In this research, differen...Chitosan/α,β-glycerophosphate （CS/α,β-GP） thermo-sensitive hydrogels presented flowable solution state at low temperature and semisolid hydrogel when the ambient temperature increased. In this research, different concentrations of metronida- zole encapsulated, CS and α,β-GP, as well as different acid solvents, were chosen to evaluate their influences on the drug release behaviors from CS/α,β-GP hydrogels. It was found that there was a sustaining release during the first 3 h followed by a plateau. SEM images showed that drugs were located both on the surface and in the interior of hydrogels. The optimal preparation conditions of this hydrogel for drug release were as follows： 1.8% （w/v） CS in HAc solvent, 5.6% （w/v） a, 13-GP and 5g/L metronidazole encapsulation. Cytotoxicity evaluation found no toxic effect. In order to control the release rate, 2.5 g/L chitosan microspheres with spherical shape and smooth surface were incorporated, and it was found that the initial release process was alleviated, while drug concentration had no obvious effect on the release rate. It could be concluded that the metronidzole release behaviors could be optimized according to practical applica- tions.展开更多
文摘AIM To assess the direct effects of ischemia onintestinal epithelial integrity. Furthermore, clinicalefforts at mitigating the effect of hypoperfusion on gutpermeability have focused on restoring gut vascularfunction.METHODS： we report that, in the Caco-2 cell modelof transepithelial transport, calcium glycerophosphate（CGP）, an inhibitor of intestinal alkaline phosphataseF3, has a significant effect to preserve transepithelialelectrical resistance （TEER） and to attenuate increasesin mannitol flux rates during hypoxia or cytokinestimulation.RESULTS： The effect was observable even at concentrationsas low as 1 μmol/L. As celiac disease is alsomarked by a loss of gut epithelial integrity, the effectof CGP to attenuate the effect of the α-gliadin peptide31-55 was also examined. In this instance, CGP exertedlittle effect of preservation of TEER, but significantlyattenuated peptide induced increase in mannitol flux.CONCLUSION： it appears that CGP treatment mightsynergize with other therapies to preserve gut epithelialintegrity.
文摘Chitosan/α,β-glycerophosphate （CS/α,β-GP） thermo-sensitive hydrogels presented flowable solution state at low temperature and semisolid hydrogel when the ambient temperature increased. In this research, different concentrations of metronida- zole encapsulated, CS and α,β-GP, as well as different acid solvents, were chosen to evaluate their influences on the drug release behaviors from CS/α,β-GP hydrogels. It was found that there was a sustaining release during the first 3 h followed by a plateau. SEM images showed that drugs were located both on the surface and in the interior of hydrogels. The optimal preparation conditions of this hydrogel for drug release were as follows： 1.8% （w/v） CS in HAc solvent, 5.6% （w/v） a, 13-GP and 5g/L metronidazole encapsulation. Cytotoxicity evaluation found no toxic effect. In order to control the release rate, 2.5 g/L chitosan microspheres with spherical shape and smooth surface were incorporated, and it was found that the initial release process was alleviated, while drug concentration had no obvious effect on the release rate. It could be concluded that the metronidzole release behaviors could be optimized according to practical applica- tions.
