In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM ric...In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements： promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.展开更多
Dear Editor,Since 2016, a growing number of mobile colistin resistance(mcr) genes have been identified and characterized (Liu et al., 2016). In addition to mcr-1 and its variants, mcr-2 to mcr-8 have now been reported...Dear Editor,Since 2016, a growing number of mobile colistin resistance(mcr) genes have been identified and characterized (Liu et al., 2016). In addition to mcr-1 and its variants, mcr-2 to mcr-8 have now been reported, which reflects a significant threat to public health and agricultural production (Sun et al.,2018).展开更多
为建立可同时检测甘薯褪绿矮化病毒（SPCSV）、甘薯G病毒（SPVG）和甘薯羽状斑驳病毒（SPFMV）多重RT-PCR检测方法,本文根据SPCSV热激蛋白基因（hsp70）及SPVG、SPFMV外壳蛋白基因（CP）基因核苷酸序列的保守区域设计特异性引物,通过引...为建立可同时检测甘薯褪绿矮化病毒（SPCSV）、甘薯G病毒（SPVG）和甘薯羽状斑驳病毒（SPFMV）多重RT-PCR检测方法,本文根据SPCSV热激蛋白基因（hsp70）及SPVG、SPFMV外壳蛋白基因（CP）基因核苷酸序列的保守区域设计特异性引物,通过引物筛选,优化多重RT-PCR反应条件,建立了能同时检测SPCSV、SPVG和SPFMV 3种病毒的多重RT-PCR检测方法。该体系能有效扩增出大小为304、433、601 bp 3个特异性片段。测序结果表明3种病毒与参考序列的一致性达94%-99%。应用建立的多重RT-PCR检测方法可稳定、准确、灵敏地同时检测单一或复合侵染3种甘薯病毒,为甘薯脱毒和病毒病诊断奠定基础。展开更多
【目的】建立鸡传染性支气管炎病毒(IBV)快速、准确的检测方法。【方法】根据GenBank中已经发表的793/B型和多株IBV的N基因和S1基因,对比分析后以N基因的保守序列设计合成了1对引物,跨度为582bp;以793/B的S1基因设计合成了1对特异型引物...【目的】建立鸡传染性支气管炎病毒(IBV)快速、准确的检测方法。【方法】根据GenBank中已经发表的793/B型和多株IBV的N基因和S1基因,对比分析后以N基因的保守序列设计合成了1对引物,跨度为582bp;以793/B的S1基因设计合成了1对特异型引物,跨度为891 bp;建立了能检测出IBV并能够同时鉴定出793/B血清型的实验室一次性PCR诊断方法。【结果】多重RT-PCR检测表明,793/B血清型可出现582和891 bp 2条核酸片段,而其他血清型只出现582 bp 1条核酸片段,新城疫病毒、传染性喉气管炎、鹅副粘病毒则无任何条带出现。【结论】所建立的TR-PCR方法具有一定的特异性,可用于IBV的快速检测和793/B血清型的临床诊断。展开更多
基金Supported by Key Special Project for Breeding and Cultivation of GMO Varieties(2011ZX08001-001,2014ZX0800101B)Special Fund from the Department of Finance of Hubei Province(2011-2015)Collaborative Breeding Project for Rice(2013-2017)
文摘In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements： promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.
文摘Dear Editor,Since 2016, a growing number of mobile colistin resistance(mcr) genes have been identified and characterized (Liu et al., 2016). In addition to mcr-1 and its variants, mcr-2 to mcr-8 have now been reported, which reflects a significant threat to public health and agricultural production (Sun et al.,2018).
文摘为建立可同时检测甘薯褪绿矮化病毒（SPCSV）、甘薯G病毒（SPVG）和甘薯羽状斑驳病毒（SPFMV）多重RT-PCR检测方法,本文根据SPCSV热激蛋白基因（hsp70）及SPVG、SPFMV外壳蛋白基因（CP）基因核苷酸序列的保守区域设计特异性引物,通过引物筛选,优化多重RT-PCR反应条件,建立了能同时检测SPCSV、SPVG和SPFMV 3种病毒的多重RT-PCR检测方法。该体系能有效扩增出大小为304、433、601 bp 3个特异性片段。测序结果表明3种病毒与参考序列的一致性达94%-99%。应用建立的多重RT-PCR检测方法可稳定、准确、灵敏地同时检测单一或复合侵染3种甘薯病毒,为甘薯脱毒和病毒病诊断奠定基础。
文摘【目的】建立鸡传染性支气管炎病毒(IBV)快速、准确的检测方法。【方法】根据GenBank中已经发表的793/B型和多株IBV的N基因和S1基因,对比分析后以N基因的保守序列设计合成了1对引物,跨度为582bp;以793/B的S1基因设计合成了1对特异型引物,跨度为891 bp;建立了能检测出IBV并能够同时鉴定出793/B血清型的实验室一次性PCR诊断方法。【结果】多重RT-PCR检测表明,793/B血清型可出现582和891 bp 2条核酸片段,而其他血清型只出现582 bp 1条核酸片段,新城疫病毒、传染性喉气管炎、鹅副粘病毒则无任何条带出现。【结论】所建立的TR-PCR方法具有一定的特异性,可用于IBV的快速检测和793/B血清型的临床诊断。
