高效抗逆转录病毒治疗法(highly active antiretroviral therapy,HAART)是当今抗人免疫缺陷病毒(HIV)感染的主要方法,随着HAART的广泛应用,诸如药物交互作用、不良反应和耐药毒株等问题也相继出现,因此人们致力于其他抗HIV药物的研究。g...高效抗逆转录病毒治疗法(highly active antiretroviral therapy,HAART)是当今抗人免疫缺陷病毒(HIV)感染的主要方法,随着HAART的广泛应用,诸如药物交互作用、不良反应和耐药毒株等问题也相继出现,因此人们致力于其他抗HIV药物的研究。gp41是HIV-1病毒表面的一种包膜糖蛋白,介导了病毒膜和宿主细胞膜间的融合,在HIV-1的侵染过程中起了关键作用。基于gp41为相关靶点的HIV-1融合抑制剂是近年来抗HIV-1药物研究的热点。概述gp41的结构及融膜机制,并对gp41为作用靶点的抗HIV-1药物的研究文献,及其研究进展做了分析。展开更多
Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced...Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.展开更多
文摘高效抗逆转录病毒治疗法(highly active antiretroviral therapy,HAART)是当今抗人免疫缺陷病毒(HIV)感染的主要方法,随着HAART的广泛应用,诸如药物交互作用、不良反应和耐药毒株等问题也相继出现,因此人们致力于其他抗HIV药物的研究。gp41是HIV-1病毒表面的一种包膜糖蛋白,介导了病毒膜和宿主细胞膜间的融合,在HIV-1的侵染过程中起了关键作用。基于gp41为相关靶点的HIV-1融合抑制剂是近年来抗HIV-1药物研究的热点。概述gp41的结构及融膜机制,并对gp41为作用靶点的抗HIV-1药物的研究文献,及其研究进展做了分析。
基金supported by grants from The National Natural Science Foundation of China(30670497)National Basic Research Program of China(2009CB930200)+1 种基金Beijing Natural Science Foundation(5072002,7082006)Research Fund for the Doctorate Program(X0015001200801)~~
文摘Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.
