Breast cancer is one of the world＇s most urgent health problems. The first symptoms of mammary malignancies are manifested only at an advanced stage with significant mortality. Detecting this disease at an early stag...Breast cancer is one of the world＇s most urgent health problems. The first symptoms of mammary malignancies are manifested only at an advanced stage with significant mortality. Detecting this disease at an early stage gives the majority of patients a better chance of survival. The aim was to search for changes in gene expression of specific tumor vascular markers like death receptor 6 （Dr6） and glycoprotein M6B （Gpm6B） in the blood of patients with breast cancer. All subjects were divided into two groups. First group with patients are with different grades of breats tumors （n = 30）. Second group consists from healthy women （n = 15）. After isolation of mRNA from blood, RT-PCR was followed by gel electrophoresis. For statistical analysis one-way ANOVA was used with Student＇s T test using GraphPad InStat software. Significant changes in mRNA levels of gene Dr6 in all grades of first stage breast cancer were detected. The mRNA levels of Dr6 showed a rising tendency from GI （116% higher value than control） to G3 （198% higher than control）. During monitoring of the mRNA level of Gpm6B, a weaker increase was observed than in Dr6. The difference in GI was only 8% higher compared with controls and 44% at G3. From our results it can be concluded that DR6 is a more suitable marker for the diagnosis of breast malignancies in the early stages than Gpm6B. In our work, a non-invasive method for more timely and precise determination of the earlier stages of breast cancer is described, which could also contribute to monitoring the effectiveness of treatment, or regression of this disease.展开更多
Colorectal carcinoma(CRC)is one of the most common cancers,and is associated with a poor dlinical outcome.The key genes and potential prognostic markers in colorectal carcinoma remain to be identfed and explored for c...Colorectal carcinoma(CRC)is one of the most common cancers,and is associated with a poor dlinical outcome.The key genes and potential prognostic markers in colorectal carcinoma remain to be identfed and explored for clinical application.DNA expression/methyl-ation profles were downloaded from the Gene Expression Omnibus(GEO)database to identify differentially expressed/methylated genes(DEGs and DEMs).A total of 255 genes and 372 genes were identified as being up-regulated and down-regulated,respectively,in GSE113513,GSE81558,and GSE89076.There were a total of 3350 hypermethylated genes and 443 hypomethylated genes identifed in GSE 48684.Twenty genes were found to be hypermethylated as well as down-regulated,and a functional enrichment analysis revealed that these genes were mainly involved in cancer-related pathways.Among these 20 genes,GPM6A,HAND2 and C2orf40 were related to poor outcomes in cancer patients based on a survival analysis.Concurrent decreases of GPM6A,HAND2 and C2orf40 protein expression were observed in highly-dif ferentiated colorectal carcinoma tissues,and higher expression levels were found in undifferentiated or minimally-differentiated colorectal carcinoma tissues.In conclusion,20 genes were found to be downregulated and hypermethylated in CRC,among which GPM6A,HAND2 and C2orf40 were explored for their potential prognostic value.展开更多
文摘Breast cancer is one of the world＇s most urgent health problems. The first symptoms of mammary malignancies are manifested only at an advanced stage with significant mortality. Detecting this disease at an early stage gives the majority of patients a better chance of survival. The aim was to search for changes in gene expression of specific tumor vascular markers like death receptor 6 （Dr6） and glycoprotein M6B （Gpm6B） in the blood of patients with breast cancer. All subjects were divided into two groups. First group with patients are with different grades of breats tumors （n = 30）. Second group consists from healthy women （n = 15）. After isolation of mRNA from blood, RT-PCR was followed by gel electrophoresis. For statistical analysis one-way ANOVA was used with Student＇s T test using GraphPad InStat software. Significant changes in mRNA levels of gene Dr6 in all grades of first stage breast cancer were detected. The mRNA levels of Dr6 showed a rising tendency from GI （116% higher value than control） to G3 （198% higher than control）. During monitoring of the mRNA level of Gpm6B, a weaker increase was observed than in Dr6. The difference in GI was only 8% higher compared with controls and 44% at G3. From our results it can be concluded that DR6 is a more suitable marker for the diagnosis of breast malignancies in the early stages than Gpm6B. In our work, a non-invasive method for more timely and precise determination of the earlier stages of breast cancer is described, which could also contribute to monitoring the effectiveness of treatment, or regression of this disease.
基金The present study was partially supported by the National Natural Science Foundation of China[grant number 81672970]the Natural Science Foundation of Jiangsu Province[grant number BK20160338]+4 种基金the projects of Suz-hou Technology Bureau[grant numbers SYS201552,SS201753 and SY52018054]the Suzhou Introduced Team of Clinical Medical Experts[grant number SZYJTD201803]the Youth Science and Technology Project of the Health Bureau of Suzhou city[grant number KJXW2017013]Jiangsu Province's Graduate Student Research Innovation Project[grant number KYCX19_1986]the Second Affiliated Hospital of Soochow University Preponderant Clinic Disci-pline Group Project Funding.
文摘Colorectal carcinoma(CRC)is one of the most common cancers,and is associated with a poor dlinical outcome.The key genes and potential prognostic markers in colorectal carcinoma remain to be identfed and explored for clinical application.DNA expression/methyl-ation profles were downloaded from the Gene Expression Omnibus(GEO)database to identify differentially expressed/methylated genes(DEGs and DEMs).A total of 255 genes and 372 genes were identified as being up-regulated and down-regulated,respectively,in GSE113513,GSE81558,and GSE89076.There were a total of 3350 hypermethylated genes and 443 hypomethylated genes identifed in GSE 48684.Twenty genes were found to be hypermethylated as well as down-regulated,and a functional enrichment analysis revealed that these genes were mainly involved in cancer-related pathways.Among these 20 genes,GPM6A,HAND2 and C2orf40 were related to poor outcomes in cancer patients based on a survival analysis.Concurrent decreases of GPM6A,HAND2 and C2orf40 protein expression were observed in highly-dif ferentiated colorectal carcinoma tissues,and higher expression levels were found in undifferentiated or minimally-differentiated colorectal carcinoma tissues.In conclusion,20 genes were found to be downregulated and hypermethylated in CRC,among which GPM6A,HAND2 and C2orf40 were explored for their potential prognostic value.
