Two kinds of new Ormosil xerogels derived from GPTMS (3-glycidyl propyl trimethoxisilane) and TEOS (tetraethoxysilane) or GPTMS and Ti(OBu)4 (tetrabutoxytitanate) were synthesized viathe sol-gel method as dye laser ho...Two kinds of new Ormosil xerogels derived from GPTMS (3-glycidyl propyl trimethoxisilane) and TEOS (tetraethoxysilane) or GPTMS and Ti(OBu)4 (tetrabutoxytitanate) were synthesized viathe sol-gel method as dye laser host. Their optical properties, microstructure characteristics and sudece laser damage thresholds have been reported. The effects of dye matrix combination on the fluorescence emission, slope efficiency, photostability and tunable bandwidth were examined.Laser oscillation was easily achieved near 620 nm with a slope efficiency of 29% from DCM dopedSiO2-Ormosil pumped by a frequency-doubled radiation from a Q-switched Nd:YAG laser. The laser wavelength was tunable over 59 nm, and the laser output energy had only a 10% reduction after 2.5× 104 pulses at a pump repetition rate of 1Hz and a pump intensity of 1展开更多
Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of the organic-inorganic biocomposite film based on the one-step electrodeposition and covalent-coupled so...Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of the organic-inorganic biocomposite film based on the one-step electrodeposition and covalent-coupled sol-gel process. The electrodeposition was performed in the solution containing functional inorganic precursor possessing the epoxy groups,γ-glycidoxypropyltrimethoxysiloxane (GPTMS),a biopolymer chitosan (CS),HRP and GOD. The covalent-coupled sol-gel process was formed by self-hydrolysis and self-condensation of GPTMS,followed by in-situ covalent cross-linking of CS,HRP and GOD through covalent reaction between amino groups and epoxy groups. The developed bienzyme biosensor presented high stability in acidic solution owing to the covalent-coupled organic-inorganic hybridization. Compared with the non-hybrid HRP-GOD/CS/Au electrode,the bienzyme biosensor of HRP-GOD/GPTMS/CS/Au showed improved sensitivity and a wider linear range for the determination of glucose. The linear response of the developed HRP-GOD/GPTMS/ CS/Au biosensor for the determination of glucose ranged from 1 to 351 μmol/L with a detection limit of 0.3 μmol/L.展开更多
文摘Two kinds of new Ormosil xerogels derived from GPTMS (3-glycidyl propyl trimethoxisilane) and TEOS (tetraethoxysilane) or GPTMS and Ti(OBu)4 (tetrabutoxytitanate) were synthesized viathe sol-gel method as dye laser host. Their optical properties, microstructure characteristics and sudece laser damage thresholds have been reported. The effects of dye matrix combination on the fluorescence emission, slope efficiency, photostability and tunable bandwidth were examined.Laser oscillation was easily achieved near 620 nm with a slope efficiency of 29% from DCM dopedSiO2-Ormosil pumped by a frequency-doubled radiation from a Q-switched Nd:YAG laser. The laser wavelength was tunable over 59 nm, and the laser output energy had only a 10% reduction after 2.5× 104 pulses at a pump repetition rate of 1Hz and a pump intensity of 1
基金Supported by the National Natural Science Foundation of China (Grant Nos. 20775039 & 20775038)
文摘Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of the organic-inorganic biocomposite film based on the one-step electrodeposition and covalent-coupled sol-gel process. The electrodeposition was performed in the solution containing functional inorganic precursor possessing the epoxy groups,γ-glycidoxypropyltrimethoxysiloxane (GPTMS),a biopolymer chitosan (CS),HRP and GOD. The covalent-coupled sol-gel process was formed by self-hydrolysis and self-condensation of GPTMS,followed by in-situ covalent cross-linking of CS,HRP and GOD through covalent reaction between amino groups and epoxy groups. The developed bienzyme biosensor presented high stability in acidic solution owing to the covalent-coupled organic-inorganic hybridization. Compared with the non-hybrid HRP-GOD/CS/Au electrode,the bienzyme biosensor of HRP-GOD/GPTMS/CS/Au showed improved sensitivity and a wider linear range for the determination of glucose. The linear response of the developed HRP-GOD/GPTMS/ CS/Au biosensor for the determination of glucose ranged from 1 to 351 μmol/L with a detection limit of 0.3 μmol/L.
