[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the ...[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.展开更多
Fasting is a popular dietary strategy because it grants numerous advantages,and redox regulation is one mecha-nism involved.However,the precise redox changes with respect to the redox species,organelles and tissues re...Fasting is a popular dietary strategy because it grants numerous advantages,and redox regulation is one mecha-nism involved.However,the precise redox changes with respect to the redox species,organelles and tissues remain unclear,which hinders the understanding of the metabolic mechanism,and exploring the precision redox map under various dietary statuses is of great significance.Twelve redox-sensitive C.elegans strains stably expressing genetically encoded redox fluorescent probes(Hyperion sensing H_(2)O_(2) and Grx1-roGFP2 sensing GSH/GSSG)in three organelles(cytoplasm,mitochondria and endoplasmic reticulum(ER))were constructed in two tissues(body wall muscle and neurons)and were confirmed to respond to redox challenge.The H_(2)O_(2) and GSSG/GSH redox changes in two tissues and three organelles were obtained by confocal microscopy during fasting,refeeding,and satiation.We found that under fasting condition,H_(2)O_(2) decreased in most compartments,except for an increase in mitochondria,while GSSG/GSH increased in the cytoplasm of body muscle and the ER of neurons.After refeeding,the redox changes in H_(2)O_(2) and GSSG/GSH caused by fasting were reversed in most organelles of the body wall muscle and neurons.In the sati-ated state,H_(2)O_(2) increased markedly in the cytoplasm,mitochondria and ER of muscle and the ER of neurons,while GSSG/GSH exhibited no change in most organelles of the two tissues except for an increase in the ER of muscle.Our study systematically and precisely presents the redox characteristics under different dietary states in living animals and provides a basis for further investigating the redox mechanism in metabolism and optimizing dietary guidance.展开更多
基金Supported by Major Innovation Program for Extraction of Plant-derived NaturalDisease-resistant Components and Industrialization(KX140564)
文摘[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.
基金supported by the National Key R&D Program(2022YFA1303000 and 2017YFA0504000)the National Natural Science Foundation of China(91849203,31900893)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB39000000).
文摘Fasting is a popular dietary strategy because it grants numerous advantages,and redox regulation is one mecha-nism involved.However,the precise redox changes with respect to the redox species,organelles and tissues remain unclear,which hinders the understanding of the metabolic mechanism,and exploring the precision redox map under various dietary statuses is of great significance.Twelve redox-sensitive C.elegans strains stably expressing genetically encoded redox fluorescent probes(Hyperion sensing H_(2)O_(2) and Grx1-roGFP2 sensing GSH/GSSG)in three organelles(cytoplasm,mitochondria and endoplasmic reticulum(ER))were constructed in two tissues(body wall muscle and neurons)and were confirmed to respond to redox challenge.The H_(2)O_(2) and GSSG/GSH redox changes in two tissues and three organelles were obtained by confocal microscopy during fasting,refeeding,and satiation.We found that under fasting condition,H_(2)O_(2) decreased in most compartments,except for an increase in mitochondria,while GSSG/GSH increased in the cytoplasm of body muscle and the ER of neurons.After refeeding,the redox changes in H_(2)O_(2) and GSSG/GSH caused by fasting were reversed in most organelles of the body wall muscle and neurons.In the sati-ated state,H_(2)O_(2) increased markedly in the cytoplasm,mitochondria and ER of muscle and the ER of neurons,while GSSG/GSH exhibited no change in most organelles of the two tissues except for an increase in the ER of muscle.Our study systematically and precisely presents the redox characteristics under different dietary states in living animals and provides a basis for further investigating the redox mechanism in metabolism and optimizing dietary guidance.
