Introduction: Genetic polymorphisms of some Glutathione S-Transferase (GST) which encode the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several patholo...Introduction: Genetic polymorphisms of some Glutathione S-Transferase (GST) which encode the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several pathologies that can progress to cancer such as Hepatitis B. This study aims to characterize the impact of the rs1695 polymorphism of GSTP1 gene among people with chronic Hepatitis B infection in Burkina Faso. Methods: rs1695 polymorphisms of GSTP1 gene genotyping was performed for 50 people infected with chronic Hepatitis B virus and 124 healthy people with the PCR-RFLP method. Conventional PCR was used for DNA amplification and Alw26I enzyme was used for enzymatic digestion. Results: The results show that the frequencies of AA, AG and GG genotypes are respectively 31.00%, 36.80% and 32.20% in general the study population with a mutation rate of 50.57%. However, the incidence of the AA, AG and GG genotypes are respectively 30.64%, 38.71% and 30.64% among people with chronic Hepatitis B virus infection and 32.00%, 32.00% and 36.00% among healthy people. In cases, the frequencies of the A and G alleles are 48.00% and 52.00% respectively, and in controls 50.00% each. No statistical difference was found by comparing genotypic and allelic frequencies between cases and controls (p > 0.05). Conclusion: Our study allowed us to determine the rate of GSTP1 rs1695 genotypes in the study population, cases and controls. From our analyses, GSTP1 rs1695 is not associated to chronic Hepatitis B virus infection in Ouagadougou.展开更多
目的系统评价中国人群中非小细胞肺癌（NSCLC）患者GSTP1基因多态性与铂类药物化学治疗（简称化疗）敏感性的相关性。方法计算机检索Cochrane Library,Pub Med,EMBASE数据库和中国生物医学文献数据库（CBM）、中国学术期刊全文数据库（C...目的系统评价中国人群中非小细胞肺癌（NSCLC）患者GSTP1基因多态性与铂类药物化学治疗（简称化疗）敏感性的相关性。方法计算机检索Cochrane Library,Pub Med,EMBASE数据库和中国生物医学文献数据库（CBM）、中国学术期刊全文数据库（CNKI）和中文科技期刊全文数据库维普（VIP）、万方等数据库中有关GSTP1基因多态性与铂类药物化疗敏感性的研究,由2名研究者独立筛选提取资料和评价质量,采用Rev Man 5.2软件进行Meta分析。结果有6篇文献符合要求,纳入研究,共527例患者。Meta分析结果显示,GSTP1 313 GG基因型（GG vs AA,OR为4.80,95%CI为1.57~14.69）和AG基因型（AG vs AA,OR为3.42,95%CI为1.99~5.86）的患者化疗有效率明显高于AA基因型患者,而且变异基因型G携带者（AG＋GG基因型）的化疗有效率明显高于野生型AA基因型患者（AG＋GG vs AA,OR为2.38,95%CI为1.61~3.53）。结论中国人群中非小细胞肺癌患者的GSTP1基因多态性与铂类药物化疗敏感性相关。展开更多
文摘Introduction: Genetic polymorphisms of some Glutathione S-Transferase (GST) which encode the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several pathologies that can progress to cancer such as Hepatitis B. This study aims to characterize the impact of the rs1695 polymorphism of GSTP1 gene among people with chronic Hepatitis B infection in Burkina Faso. Methods: rs1695 polymorphisms of GSTP1 gene genotyping was performed for 50 people infected with chronic Hepatitis B virus and 124 healthy people with the PCR-RFLP method. Conventional PCR was used for DNA amplification and Alw26I enzyme was used for enzymatic digestion. Results: The results show that the frequencies of AA, AG and GG genotypes are respectively 31.00%, 36.80% and 32.20% in general the study population with a mutation rate of 50.57%. However, the incidence of the AA, AG and GG genotypes are respectively 30.64%, 38.71% and 30.64% among people with chronic Hepatitis B virus infection and 32.00%, 32.00% and 36.00% among healthy people. In cases, the frequencies of the A and G alleles are 48.00% and 52.00% respectively, and in controls 50.00% each. No statistical difference was found by comparing genotypic and allelic frequencies between cases and controls (p > 0.05). Conclusion: Our study allowed us to determine the rate of GSTP1 rs1695 genotypes in the study population, cases and controls. From our analyses, GSTP1 rs1695 is not associated to chronic Hepatitis B virus infection in Ouagadougou.
文摘目的系统评价中国人群中非小细胞肺癌（NSCLC）患者GSTP1基因多态性与铂类药物化学治疗（简称化疗）敏感性的相关性。方法计算机检索Cochrane Library,Pub Med,EMBASE数据库和中国生物医学文献数据库（CBM）、中国学术期刊全文数据库（CNKI）和中文科技期刊全文数据库维普（VIP）、万方等数据库中有关GSTP1基因多态性与铂类药物化疗敏感性的研究,由2名研究者独立筛选提取资料和评价质量,采用Rev Man 5.2软件进行Meta分析。结果有6篇文献符合要求,纳入研究,共527例患者。Meta分析结果显示,GSTP1 313 GG基因型（GG vs AA,OR为4.80,95%CI为1.57~14.69）和AG基因型（AG vs AA,OR为3.42,95%CI为1.99~5.86）的患者化疗有效率明显高于AA基因型患者,而且变异基因型G携带者（AG＋GG基因型）的化疗有效率明显高于野生型AA基因型患者（AG＋GG vs AA,OR为2.38,95%CI为1.61~3.53）。结论中国人群中非小细胞肺癌患者的GSTP1基因多态性与铂类药物化疗敏感性相关。
