Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explan...Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.展开更多
To estimate genetic variation in rhizome lotus(Nelumbo nucifera Gaertn.ssp.nucifera) germplasms in China,a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed.The RAPD(randomly ampl...To estimate genetic variation in rhizome lotus(Nelumbo nucifera Gaertn.ssp.nucifera) germplasms in China,a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed.The RAPD(randomly amplified polymorphic DNA) marker was employed.The selected 17 random primers detected 139 polymorphic alleles out of a total 207(67.15%).Nei's gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202,h = 0.1937,I = 0.2982 and the gene flow among all regions was Nm = 5.5742.The UPGMA dendrogram clustered all 94 germplasms into two clusters:One contained eight commercial cultivars and major landraces,and the other included the wild and some special landraces from five regions,and the PCA analysis exhibited the similar result.Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern,northern and central China.Predominant proportion of genetic variation(95.61%) was found significant within rather than among(4.39%) regions,as revealed by AMOVA analysis.The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances,though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis.A relatively high genetic diversity and gene flow resided in the root lotus germplasms;about 96% of the variation was found within region;accessions from southwest and eastern China have higher genetic diversity than those from the southern,northern and central China.展开更多
[Objectives] This study was conducted to investigate the genetic diversity of 20 lotus( Nelumbo nucifera Gaertn) samples. [Methods]On this optimal ISSR amplification system,16 primers were screened with good polymorph...[Objectives] This study was conducted to investigate the genetic diversity of 20 lotus( Nelumbo nucifera Gaertn) samples. [Methods]On this optimal ISSR amplification system,16 primers were screened with good polymorphism,and the DNA was used to amplify the 20 plant samples. [Results] The 16 primers produced 225 loci,of which 170 were polymorphic,and the polymorphic loci percentage was up to 75. 56%. The genetic similarity coefficients between the 20 varieties ranged from 0. 577 8 to 0. 951 1,which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups,the first of which included Baiyangdian red lotus and Donggua lotus,and other varieties was included in the second group. [Conclusions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.展开更多
In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings,research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings ...In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings,research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30. 9% to 83. 0%. Among them,the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones,and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.展开更多
To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fer...To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.展开更多
基金Supported by Grant from Universitas Airlangga,Surabaya,Indonesia with Grant No.8714/UN3/KR/2013
文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.
文摘To estimate genetic variation in rhizome lotus(Nelumbo nucifera Gaertn.ssp.nucifera) germplasms in China,a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed.The RAPD(randomly amplified polymorphic DNA) marker was employed.The selected 17 random primers detected 139 polymorphic alleles out of a total 207(67.15%).Nei's gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202,h = 0.1937,I = 0.2982 and the gene flow among all regions was Nm = 5.5742.The UPGMA dendrogram clustered all 94 germplasms into two clusters:One contained eight commercial cultivars and major landraces,and the other included the wild and some special landraces from five regions,and the PCA analysis exhibited the similar result.Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern,northern and central China.Predominant proportion of genetic variation(95.61%) was found significant within rather than among(4.39%) regions,as revealed by AMOVA analysis.The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances,though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis.A relatively high genetic diversity and gene flow resided in the root lotus germplasms;about 96% of the variation was found within region;accessions from southwest and eastern China have higher genetic diversity than those from the southern,northern and central China.
基金Supported by the Education Department Project of Fujian Province(JB11039)
文摘[Objectives] This study was conducted to investigate the genetic diversity of 20 lotus( Nelumbo nucifera Gaertn) samples. [Methods]On this optimal ISSR amplification system,16 primers were screened with good polymorphism,and the DNA was used to amplify the 20 plant samples. [Results] The 16 primers produced 225 loci,of which 170 were polymorphic,and the polymorphic loci percentage was up to 75. 56%. The genetic similarity coefficients between the 20 varieties ranged from 0. 577 8 to 0. 951 1,which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups,the first of which included Baiyangdian red lotus and Donggua lotus,and other varieties was included in the second group. [Conclusions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.
基金Supported by Forestry Science and Technology Planning Project of Hunan Province,China(XLK201511)
文摘In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings,research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30. 9% to 83. 0%. Among them,the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones,and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.
基金financial support they received from the National Key R&D Program of China(2020YFD1000300)the earmarked fund for China Agriculture Research System(CARS-24)the HighLevel Talent Support Plan(Lv-Yang-Jin-Feng),Yangzhou,China。
文摘To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.
