The interaction of Gallocyanine(Gal) with DNA was studied by using UV spectra and circular dichroism(CD) spectrum methods. Gal and DNA strongly interacted on each other in acid solutions. The intrinsic binding constan...The interaction of Gallocyanine(Gal) with DNA was studied by using UV spectra and circular dichroism(CD) spectrum methods. Gal and DNA strongly interacted on each other in acid solutions. The intrinsic binding constant( K ) and the binding numbers( C DNA / C Gal ) of Gal and DNA are 2\^25×10 4 mol -1 and 2\^5, respectively. The influence of pH on the interaction of Gal and DNA was also studied.展开更多
A novel kinetic procedure for the determination of trace iron(Ⅲ) was proposed based on the aerial oxidation of Gallocyanine by oxygen dissolved in water at pH 7 0. The calibration graph is linear for 0 05—0.6 μg...A novel kinetic procedure for the determination of trace iron(Ⅲ) was proposed based on the aerial oxidation of Gallocyanine by oxygen dissolved in water at pH 7 0. The calibration graph is linear for 0 05—0.6 μg/ml, and the detection limit is 0 01μg/ml (Fe). The present procedure is selective for iron(Ⅲ) and very easy to control compared with kinetic procedures for iron. It had been used to the determination of trace iron in environmental samples, and the results were satisfactory.展开更多
基金Supported by the National Natural Science Foundation of China.
文摘The interaction of Gallocyanine(Gal) with DNA was studied by using UV spectra and circular dichroism(CD) spectrum methods. Gal and DNA strongly interacted on each other in acid solutions. The intrinsic binding constant( K ) and the binding numbers( C DNA / C Gal ) of Gal and DNA are 2\^25×10 4 mol -1 and 2\^5, respectively. The influence of pH on the interaction of Gal and DNA was also studied.
文摘A novel kinetic procedure for the determination of trace iron(Ⅲ) was proposed based on the aerial oxidation of Gallocyanine by oxygen dissolved in water at pH 7 0. The calibration graph is linear for 0 05—0.6 μg/ml, and the detection limit is 0 01μg/ml (Fe). The present procedure is selective for iron(Ⅲ) and very easy to control compared with kinetic procedures for iron. It had been used to the determination of trace iron in environmental samples, and the results were satisfactory.
