Comparison ofβ-Amyloid Plaque Labeling Methods:Antibody Staining,Gallyas Silver Staining,and Thioflavin-S Staining

Objective To evaluate senile plaque formation and compare the sensitivity of three differentβ-amyloid(Aβ)labeling methods(antibody staining,Gallyas silver staining,and thioflavin-S staining)to detect Aβdeposition.Methods APPswe/PSEN1dE9 transgenic mice(APP/PS1)of different ages were used to examine spatiotemporal changes in Aβplaque deposition.Antibody staining,Gallyas silver staining,and thioflavin-S staining were used to detect Aβplaque deposition in the same brain region of adjacent slices from model mice,and the results were compared.Results With aging,Aβplaques first appeared in the cortex and then the deposition increased throughout the whole brain.Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining(P<0.05).Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining(P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer’s disease could mimick the progress of Aβplaques occurred in patients with Alzheimer’s disease.Antibody detection of Aβdeposition may be more sensitive than chemical staining methods.

阿尔茨海默氏型老年性痴呆压部后区皮质的病理变化

压部后区属于中间皮质，在机能上属于边缘系统，要在记忆和学习上起着重要的作用。研究人员采用Gallyas镀银技术染色和Campbel技术对11个来自病理解剖后立即固定在4%福尔马林水溶液中的脑进行了关于阿尔茨海默氏型老年性痴呆的研究，在本研究中，Gallyas制备在所有痴呆个体的压部后区皮质显示大量阿尔茨海默氏神经原纤维变化，而在非痴呆个体的这个区中几乎没有这种变化，因此，我们认为Gallysa方法是研究阿尔茨海默氏改变的1种良好技术，Gallyas方法不但能显示其他镀银技术显示的老年斑及神经原纤维缠结，而且还能显示其他镀银技术不能浸染的神经毡细丝，Campbell制备在1个非痴呆个体的压部后区中显示大量淀粉样蛋白沉着，研究证明Campbell制备中的嗜银物质的分布模式类似用抗纯化的淀粉样蛋白抗体免疫染色材料的分布模式，这表明Campbell技术是特异性的，在老年斑常出现的结构中，淀粉样蛋白的沉着可能是老年斑形成的第1阶段，我们的结果也表明，淀粉样蛋白的沉着出现在细胞内病理变化和临床症状出现之前。