Objective： To study the effect of Gan （肝）-Pi （脾） regulatory needling （GPRN） in treating chloasma and its influences on female sex hormones, superoxide dismutase （SOD）, lipid peroxide （LPO） and melanocyte-...Objective： To study the effect of Gan （肝）-Pi （脾） regulatory needling （GPRN） in treating chloasma and its influences on female sex hormones, superoxide dismutase （SOD）, lipid peroxide （LPO） and melanocyte-stimulating hormone （MSH）. Methods： Ninety chloasma patients were equally randomized to three groups, the treatment group treated with GPRN, the control group treated with conventional Western medicine and the blank group untreated. Changes in the scores of skin lesion （area and color） and symptom, as well as blood levels of female sex hormones, MSH, SOD and LPO were Observed and compared after 3 months of treatment. Results： In the treatment group, the scores of skin lesion area and color were reduced from 2.76± 0.96 and 2.48 ± 0.78 before treatment to 1.42 ± 0.42 and 1.03± 0.41 after treatment, respectively, while in the control group they were from 2.78 ± 1.06 and 2.53 ± 0.88 to 1.58± 1.23 and 1.28 ± 0.96, respectively, all showing significant changes （P〈0.05）; the scores were insignificantly changed in the blank group （P〉0.05）. At the same time, the score of symptoms in the treatment group significantly improved after treatment （P〈0.05）, significantly different from that of the other two groups. Comparison of female sex hormones among groups showed no significant differences either before or after treatment. The level of LPO decreased and SOD increased in both the treatment group and the control group significantly （all P〈0.05）, but significant lowering of MSH was only seen in the treatment group （P〈0.05）. Conclusions： GPRN can effectively lessen the size and lighten the color of chloasma, improve the accompanying symptoms in patients and decrease LPO and MSH levels and increase the SOD level, but will not affect the level of the female sex hormones.展开更多
目的观察慢性乙肝病毒(hepatitis B virus,HBV)感染不同免疫状态肝郁脾虚证和肝胆湿热证患者外周血树突状细胞(dendritic cells,DCs)的功能变化,了解慢性HBV感染两证型的免疫表达特点,为中医辨证提供客观化依据。方法选取40例慢性HBV感...目的观察慢性乙肝病毒(hepatitis B virus,HBV)感染不同免疫状态肝郁脾虚证和肝胆湿热证患者外周血树突状细胞(dendritic cells,DCs)的功能变化,了解慢性HBV感染两证型的免疫表达特点,为中医辨证提供客观化依据。方法选取40例慢性HBV感染患者,根据免疫状态分为两组,慢性乙型肝炎组(免疫清除状态)20例,其中肝郁脾虚证10例,肝胆湿热证10例;慢性HBV携带组(免疫耐受状态)20例,其中肝郁脾虚证10例,肝胆湿热证10例;另选取湖南中医药大学研究生志愿者体检健康者10名为健康对照组,分别对其外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)进行体外培养,以倒置显微镜和电镜观察DCs外部形态和超微结构,检测DCs的表面分子HLA-DR、CD80、CD86、CD1a的表达及白细胞介素-12(IL-12)水平,采用MTT法检测其同种异体混合淋巴细胞增殖能力以比较DCs在慢性HBV感染不同阶段两证型中的功能特点,分析两证型间DCs各指标的差异。结果与健康对照组比较,慢性HBV携带组两个证型患者CD86、CD80及HLA-DR表达率降低,慢性乙型肝炎组两个证型患者CD80表达率降低,差异均有统计学意义(P<0.05)。患者组肝郁脾虚证患者CD86、HLA-DR低于肝胆湿热证,慢性HBV携带组肝郁脾虚证CD80低于肝胆湿热证,差异均有统计学意义(P<0.05)。患者组IL-12及T淋巴细胞增殖能力低于健康对照组,差异有统计学意义(P<0.05)。慢性乙型肝炎组肝郁脾虚证患者IL-12及T淋巴细胞增殖能力低于肝胆湿热证,差异有统计学意义(P<0.05)。结论慢性HBV感染不同免疫状态肝郁脾虚证与肝胆湿热证患者外周血DCs功能存在差异。DCs表型及功能检测在疾病的同一阶段可为中医辨证分型提供客观化指标,反映两证型免疫表达的特点及免疫学内涵。展开更多
基金Supported by Project of Traditional Chinese Medicine Science and Technology of Zhejiang Province(No.2006C159)
文摘Objective： To study the effect of Gan （肝）-Pi （脾） regulatory needling （GPRN） in treating chloasma and its influences on female sex hormones, superoxide dismutase （SOD）, lipid peroxide （LPO） and melanocyte-stimulating hormone （MSH）. Methods： Ninety chloasma patients were equally randomized to three groups, the treatment group treated with GPRN, the control group treated with conventional Western medicine and the blank group untreated. Changes in the scores of skin lesion （area and color） and symptom, as well as blood levels of female sex hormones, MSH, SOD and LPO were Observed and compared after 3 months of treatment. Results： In the treatment group, the scores of skin lesion area and color were reduced from 2.76± 0.96 and 2.48 ± 0.78 before treatment to 1.42 ± 0.42 and 1.03± 0.41 after treatment, respectively, while in the control group they were from 2.78 ± 1.06 and 2.53 ± 0.88 to 1.58± 1.23 and 1.28 ± 0.96, respectively, all showing significant changes （P〈0.05）; the scores were insignificantly changed in the blank group （P〉0.05）. At the same time, the score of symptoms in the treatment group significantly improved after treatment （P〈0.05）, significantly different from that of the other two groups. Comparison of female sex hormones among groups showed no significant differences either before or after treatment. The level of LPO decreased and SOD increased in both the treatment group and the control group significantly （all P〈0.05）, but significant lowering of MSH was only seen in the treatment group （P〈0.05）. Conclusions： GPRN can effectively lessen the size and lighten the color of chloasma, improve the accompanying symptoms in patients and decrease LPO and MSH levels and increase the SOD level, but will not affect the level of the female sex hormones.
