Gandou decoction(GDD),a well-known traditional Chinese medicine(TCM)formula,has been widely used for decades to treat Wilson's disease(WD)in China due to its remarkable clinical effects.However,the chemical consti...Gandou decoction(GDD),a well-known traditional Chinese medicine(TCM)formula,has been widely used for decades to treat Wilson's disease(WD)in China due to its remarkable clinical effects.However,the chemical constituents of GDD still remain unclear because of their complexity.In this work,a reliable and sensitive strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MSE)and UNIFI informatics platform was applied to investigate the chemical components in GDD.In total,96 compounds including anthraquinones,alkaloids,protostane triterpenoids,flavonoids,triterpenoid saponins,tannins,curcuminoids,etc,were identified or tentatively characterized from GDD by comparing their retention time,accurate mass within 5 ppm error and MSE fragmentation patterns.Among them,eleven compounds were confirmed unambiguously with reference standards.Representative compounds in different chemical structure types were analyzed in fragmentation patterns and characteristic ions.Moreover,to better understand the chemical contribution of individual herbs to the whole decoction,the corresponding each herb in GDD was also detected.This study developed a rapid method for characterizing the chemical constituents in GDD,which could not only be used for chemical standardization and quality control,but also be helpful for further research of GDD in vivo.展开更多
Objective: To investigate the therapeutic efficacy of Gandou Tablet Ⅰ (肝豆片Ⅰ号) in treating hepatolenticular degeneration (HLD) and its effect on urinary trace element in 24 hours. Methods: Thirty four patients ...Objective: To investigate the therapeutic efficacy of Gandou Tablet Ⅰ (肝豆片Ⅰ号) in treating hepatolenticular degeneration (HLD) and its effect on urinary trace element in 24 hours. Methods: Thirty four patients with HLD took Gandou Tablet Ⅰ for 4 weeks, their changes of symptoms, signs and daily life pattern were observed closely, and the levels of urinary trace element in 24 hours before the therapy and every week after initiation of treatment were measured. Results: The total effective rate reached 70.59%, with markedly effective rate being 8.82%.The therapeutic effect was much better in the child and mild patients. After treatment, the daily urinary copper output was obviously increased every week compared with that before ( P < 0.01 ). Although it was highly negative linear correlated with the duration of treatment (γ=-0.96, P < 0.05 ), it has insignificant difference between any two weekly excretion of urinary copper per 24 hours after treatment ( P >0.05). So its effect on urinary copper excretion was not decreased. Conclusion: Gandou Tablet Ⅰ could increase the output of urinary copper, hence it was effective in treating HLD without apparent toxic or side reaction.展开更多
基金This research was supported by the National Natural Science Foundation of China(No:81872976,81873038,81303245)Key Project of the National Science Fund of Anhui Province(No:KJ2016A398)+1 种基金Key Project Foundation of Oversea Visiting and Research for the Excellent Young and Middle-aged Faculties in Universities of Anhui Province(No:gxgwfx2018042)Primary Research&Development Plan of Anhui Province(No:201904a07020051).
文摘Gandou decoction(GDD),a well-known traditional Chinese medicine(TCM)formula,has been widely used for decades to treat Wilson's disease(WD)in China due to its remarkable clinical effects.However,the chemical constituents of GDD still remain unclear because of their complexity.In this work,a reliable and sensitive strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MSE)and UNIFI informatics platform was applied to investigate the chemical components in GDD.In total,96 compounds including anthraquinones,alkaloids,protostane triterpenoids,flavonoids,triterpenoid saponins,tannins,curcuminoids,etc,were identified or tentatively characterized from GDD by comparing their retention time,accurate mass within 5 ppm error and MSE fragmentation patterns.Among them,eleven compounds were confirmed unambiguously with reference standards.Representative compounds in different chemical structure types were analyzed in fragmentation patterns and characteristic ions.Moreover,to better understand the chemical contribution of individual herbs to the whole decoction,the corresponding each herb in GDD was also detected.This study developed a rapid method for characterizing the chemical constituents in GDD,which could not only be used for chemical standardization and quality control,but also be helpful for further research of GDD in vivo.
文摘Objective: To investigate the therapeutic efficacy of Gandou Tablet Ⅰ (肝豆片Ⅰ号) in treating hepatolenticular degeneration (HLD) and its effect on urinary trace element in 24 hours. Methods: Thirty four patients with HLD took Gandou Tablet Ⅰ for 4 weeks, their changes of symptoms, signs and daily life pattern were observed closely, and the levels of urinary trace element in 24 hours before the therapy and every week after initiation of treatment were measured. Results: The total effective rate reached 70.59%, with markedly effective rate being 8.82%.The therapeutic effect was much better in the child and mild patients. After treatment, the daily urinary copper output was obviously increased every week compared with that before ( P < 0.01 ). Although it was highly negative linear correlated with the duration of treatment (γ=-0.96, P < 0.05 ), it has insignificant difference between any two weekly excretion of urinary copper per 24 hours after treatment ( P >0.05). So its effect on urinary copper excretion was not decreased. Conclusion: Gandou Tablet Ⅰ could increase the output of urinary copper, hence it was effective in treating HLD without apparent toxic or side reaction.
