Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension...Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.展开更多
The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent accordi...The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent according to some reports abroad. It had been tested to drive oil by steam inoil--field. In China the first test of cross-well acoustic tomography was performed in Fuyu oilfield, Jinn Province, in Dec. 1992. Through data processing in detail, an acoustic tomogram wasobtained, in which the distribution and inclination of oil layers between two boreholes are well revealed and coincident with the logging results. SOme technical problems existed in this experiment are POinted.展开更多
[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in t...[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in the west of Jilin Province, water resource balance between the amount of oil-field water supply and ecological water requirement in the constructed wetland irrigated by oil-field water during 2001-2010 was investigated firstly. Afterwards, based on 3S technology, the partition and dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation in 2001, 2006, 2008 and 2010 were analyzed. [Result] The annual ecological water requirement of the constructed wetland from 2003 to 2010 varied from 1.62×106 to 2.24×106 m3, and the annual amount of oil-field water supply in the region changed from 2.12×106 to 2.84×106 m3, which showed that the supply amount of oil-field water could meet the basic ecological water requirement of the constructed wetland. Meanwhile, compared with 2001, the areas of water region and paddy field in 2010 increased by 2.3 and 10.0 times, and the areas of forest and marsh rose by 40.15% and 29.5.0% respectively. [Conclusion] Water shortage and ecological environment problem of arid and semi-arid areas had been improved by oil-field water irrigation.展开更多
This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of ...This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of oil well heads,long-distance oil transmission lines,large-scale storage tanks, various kinds of oil station and stores, and the applications of welding technology in the typical construction of oil field. Finally, it presents suggestions on the future development of welding technology in oil-field construction for discussion.展开更多
Applications, theoretical analysis and numerical methods are introduced for the simulation of mechanical models and principles of the porous flow in high temperature, high salt, complicated geology and large-scale res...Applications, theoretical analysis and numerical methods are introduced for the simulation of mechanical models and principles of the porous flow in high temperature, high salt, complicated geology and large-scale reservoirs in this paper. Considering petroleum geology, geochemistry, computational permeation fluid mechanics and computer technology, we state the models of permeation fluid mechanics and put forward a sequence of implicit upwind difference iteration schemes based on refined fractional steps of the upstream, which can compute the pressures, the saturation and the concentrations of different chemistry components. A type of software applicable in major industries has been completed and carried out in numerical analysis and simulations of oil extraction in Shengli Oil-field, which brings huge economic benefits and social benefits. This software gives many characters: spatial steps are taken as ten meters, the number of nodes is up to hundreds of thousands and simulation time period can be tens of years and the high-order accuracy can be promised in numerical data. Precise analysis is present for simplified models of this type and that provides a tool to solve the international famous problem.展开更多
文摘Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.
文摘The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent according to some reports abroad. It had been tested to drive oil by steam inoil--field. In China the first test of cross-well acoustic tomography was performed in Fuyu oilfield, Jinn Province, in Dec. 1992. Through data processing in detail, an acoustic tomogram wasobtained, in which the distribution and inclination of oil layers between two boreholes are well revealed and coincident with the logging results. SOme technical problems existed in this experiment are POinted.
基金Supported by 2007 Environmental Protection Project of Jilin Provincial Department of Environmental Protection(2007-09)
文摘[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in the west of Jilin Province, water resource balance between the amount of oil-field water supply and ecological water requirement in the constructed wetland irrigated by oil-field water during 2001-2010 was investigated firstly. Afterwards, based on 3S technology, the partition and dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation in 2001, 2006, 2008 and 2010 were analyzed. [Result] The annual ecological water requirement of the constructed wetland from 2003 to 2010 varied from 1.62×106 to 2.24×106 m3, and the annual amount of oil-field water supply in the region changed from 2.12×106 to 2.84×106 m3, which showed that the supply amount of oil-field water could meet the basic ecological water requirement of the constructed wetland. Meanwhile, compared with 2001, the areas of water region and paddy field in 2010 increased by 2.3 and 10.0 times, and the areas of forest and marsh rose by 40.15% and 29.5.0% respectively. [Conclusion] Water shortage and ecological environment problem of arid and semi-arid areas had been improved by oil-field water irrigation.
文摘This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of oil well heads,long-distance oil transmission lines,large-scale storage tanks, various kinds of oil station and stores, and the applications of welding technology in the typical construction of oil field. Finally, it presents suggestions on the future development of welding technology in oil-field construction for discussion.
文摘Applications, theoretical analysis and numerical methods are introduced for the simulation of mechanical models and principles of the porous flow in high temperature, high salt, complicated geology and large-scale reservoirs in this paper. Considering petroleum geology, geochemistry, computational permeation fluid mechanics and computer technology, we state the models of permeation fluid mechanics and put forward a sequence of implicit upwind difference iteration schemes based on refined fractional steps of the upstream, which can compute the pressures, the saturation and the concentrations of different chemistry components. A type of software applicable in major industries has been completed and carried out in numerical analysis and simulations of oil extraction in Shengli Oil-field, which brings huge economic benefits and social benefits. This software gives many characters: spatial steps are taken as ten meters, the number of nodes is up to hundreds of thousands and simulation time period can be tens of years and the high-order accuracy can be promised in numerical data. Precise analysis is present for simplified models of this type and that provides a tool to solve the international famous problem.
