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Determination of ganoderic acids in rat plasma after oral administration of total triterpenoids from Ganoderma lucidum 被引量:2
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作者 薛彬彬 郭晓宇 车庆明 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第3期236-240,共5页
To investigate the absorption of total triterpenoids from Ganoderma lucidum in rats. HPLC-DAD and LC-MS methods were used to identify ganoderic acids in rat plasma after oral administration of total triterpenoids from... To investigate the absorption of total triterpenoids from Ganoderma lucidum in rats. HPLC-DAD and LC-MS methods were used to identify ganoderic acids in rat plasma after oral administration of total triterpenoids from G. lucidum by comparing their HPLC retention behaviors, UV absorption spectra, and mass spectra with authentic samples. Five ganoderic acids, ganoderic acid C2, C6, G, B and A were simultaneously detected in rat plasma. Ganoderic acids can be directly absorbed into circulation after oral administration of total triterpenoids from G. lucidum in rats. 展开更多
关键词 Ganoderma lucidum TRITERPENOIDS ganoderic acids LC-MS HPLC-DAD
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Rapid Simultaneous Determination of Four Ganoderic Acids in Ganoderma(Chinese Name:Lingzhi)by Direct Infusion-Multiple Reaction Monitoring Cubed 被引量:1
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作者 Nian Wu Bo Peng +5 位作者 Ting Li Pengfei Tu Shengpeng Wang Bo Li Wenjing Liu Yuelin Song 《Journal of Analysis and Testing》 EI CSCD 2024年第1期52-62,共11页
Attributing to the rapid demand expansion for the edible medicinal materials in the market,the limited throughput of highperformance liquid chromatography-multiple reaction monitoring(HPLC-MRM)cannot fully address the... Attributing to the rapid demand expansion for the edible medicinal materials in the market,the limited throughput of highperformance liquid chromatography-multiple reaction monitoring(HPLC-MRM)cannot fully address the measurement workload for a huge number of testing samples.Hence,it is urgent to pursue more efficient approaches for the quality evaluation.Because of the greater selectivity of MRM cubed(MRM^(3))over MRM,there might be a chance to omit the time-intensive LC separation.In current study,we attempted to develop a direct infusion(DI)-MRM^(3) program,and the applicability was thereafter assessed through simultaneous determination of four ganoderic acids(GAs)in one of the most famous tonic herbal medicines namely Ganoderma(Chinese name:Lingzhi).Primary parameters such as Q1>Q3>QLIT ion transitions,collision energy(CE),and excitation energy were optimized by programming online energy-resolved mass spectrometry with authentic compounds.A single DI-MRM measurement merely costed four minutes,and in spite of the wide occurrences of isomers,satisfactory selectivity was achieved.Method validation assays demonstrated the method to be sensitive,precise,accurate,and reproducible.The quantitative results from DI-MRM^(3) were also justified by conducting LC-MRM measurements in parallel.Significant differences occurred for the content patterns between the two original sources namely Ganoderma lucidum and G.sinense,and,moreover,either cultivar or harvest time showed dramatical influence on the quantitative features of the four targeted GAs.More importantly,DI-MRM3 is a meaningful analytical option for rapid quantitative analysis of herbal medicines,because of the comparable reliability,nonetheless,less consumptions of both measurement time and solvent,compared with LC-MRM. 展开更多
关键词 Direct infusion Multiple reaction monitoring cubed High-throughput quantitative analysis ganoderic acids GANODERMA
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Ganoderic acid A ameliorates renalfibrosis by suppressing the expression of NPC1L1
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作者 TIANYUN HAN ZHONG LI +1 位作者 LUONING ZHANG LINSHEN XIE 《BIOCELL》 SCIE 2024年第11期1625-1638,共14页
Objective: The study aimed to explore the protective mechanism of Ganoderic acid A (GAA) in renal fibrosisand to verify that GAA can ameliorate renal fibrosis by regulating the Niemann-pick C1-like 1 (NPC1L1) gene. Meth... Objective: The study aimed to explore the protective mechanism of Ganoderic acid A (GAA) in renal fibrosisand to verify that GAA can ameliorate renal fibrosis by regulating the Niemann-pick C1-like 1 (NPC1L1) gene. Methods:Transforming growth factor beta1 (TGF-β1) was used to treat Human Kidney-2 (HK-2) cells to establish a renal fibrosismodel. The differentially expressed genes in the control (CTRL) group, TGF-β1 group, and TGF-β1 + GAA group werescreened via transcriptome sequencing technology and verified by qPCR and Western blot experiments. The NPC1L1gene overexpression plasmid was constructed. The expression levels of N-cad, E-cad, and Slug-related proteins inCTRL, TGF-β1, TGF-β1+GAA (25 μg/mL), and TGF-β1+GAA (25 μg/mL) + NPC1L1 Overexpression (OE) groupswere detected by qPCR and Western blot analysis. Western blot analysis was used to identify the extracellular matrixassociated proteins Tenascin-C, α-SMA, and fibrosis-related protein Collagen I. Fibrosis marker protein Fibronectinwas detected and quantified by immunofluorescence. Results: Transcriptomic sequencing revealed that TGF-β1stimulation led to 267 differentially regulated genes, with 118 up-regulated and 149 down-regulated, while furthermodulation of 213 genes, comprising 112 up-regulated and 101 down-regulated genes, was observed in the GAAintervention group. The target gene in these processes was found to be NPC1L1 by investigations using GeneOntology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). qPCR and Western blot resultsconfirmed that TGF-β1 increased NPC1L1 expression, which was attenuated by GAA. Additionally, TGF-β1upregulated N-cad and Slug. However, GAA reversed this effect and NPC1L1 overexpression partially rescued theGAA effect. TGF-β1 also decreased E-cad expression, reversed by GAA, and NPC1L1 overexpression antagonized thisreversal. Furthermore, TGF-β1 promoted Collagen I, α-SMA, and Tenascin-C expression, and GAA reduced theselevels, effects that were reversed by NPC1L1 overexpression. Immunofluorescence results showed that TGF-β1increased fibronectin expression, which was decreased by GAA, and increased by NPC1L1 overexpression.Conclusion: GAA ameliorates renal fibrosis by antagonizing NPC1L1 gene expression inhibiting epithelialmesenchymal transition and reducing extracellular matrix formation. 展开更多
关键词 ganoderic acid A NPC1L1 Epithelial-mesenchymal transition Renalfibrosis TRANSCRIPTOMICS TGF-Β
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Studies on the Triterpenoid Constituents of the Spores from Ganoderma lucidum Karst 被引量:3
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作者 陈若芸 于德泉 《Journal of Chinese Pharmaceutical Sciences》 CAS 1993年第2期91-96,共6页
Five compounds were isolated from the ether-soluble fraction of the spores of Gano- derma lucidum.On the basis of their chemical properties and spectral data(MS,UV,IR,~1H and ^(13)CNMR),they were identified as 3,7,11,... Five compounds were isolated from the ether-soluble fraction of the spores of Gano- derma lucidum.On the basis of their chemical properties and spectral data(MS,UV,IR,~1H and ^(13)CNMR),they were identified as 3,7,11,12,15,23-hexaoxo-5α-lanosta-8-en-26-oic acid(Ⅰ),gano- deric acid B(Ⅱ),C(Ⅲ),D(Ⅳ)and ganodermanontriol(Ⅴ).Compound Ⅰ is a new natural product, named ganosporeric acid A.Compounds Ⅱ,Ⅲ,Ⅳ and Ⅴ are known compounds and were obtained for the first time from the spores of Ganoderma lucidum.Pharmacological experiments showed that ganosporeric acid A has an activity for lowering the levels GPT in mice with liver injury by CCl_4 and GaNI and exhibits heptoprotective effects. 展开更多
关键词 Spores of Ganoderma lucidum Ganosporeric acid A ganoderic acid B C E Ganodermanontriol
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Hydrodynamic Shear Stress Affects Cell Growth and Metabolite Production by Medicinal Mushroom Ganoderma lucidum 被引量:3
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作者 龚海刚 钟建江 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2005年第3期426-428,共3页
By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at a... By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale. 展开更多
关键词 Ganoderma lucidum mushroom cultures shear effect Ganoderma polysaccharide ganoderic acid BIOREACTOR
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Quantitative determination of ganoderic acid T in rat plasma by a sensitive RP-HPLC method and its application in pharmacokinetic studies 被引量:2
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作者 陈香 陶少林 +2 位作者 高峰 李英波 钟建江 《Journal of Chinese Pharmaceutical Sciences》 CAS 2010年第3期209-213,共5页
A selective and sensitive HPLC method has been developed and validated for the quantification of ganoderic acid T in rat plasma. A reverse-phase column was used with UV detection at 245 nm. The mobile phase consisted ... A selective and sensitive HPLC method has been developed and validated for the quantification of ganoderic acid T in rat plasma. A reverse-phase column was used with UV detection at 245 nm. The mobile phase consisted of methanol-water-acetic acid (95.5:4.5:0.5, v/v/v) run at a flow rate of 1 mL/min. Testosterone propionate was used as the internal standard. The standard curve was linear over the range of 0.05-50 ~tg/mL in rat plasma with a linear correlation coefficient greater than 0.999. The lower limit of quantification of this assay was 20 ng/mL. The recoveries of samples at 0.05, 2 and 40 μg/mL were 97.6%, 98.4% and 103.2%, respectively. The relative standard deviations of intra- and inter-day assay variations were less than 8.2%. The usefulness of the assay was confirmed by the successful analysis of plasma samples from a pharmacokinetics study in rats. Following a single dose of ganoderic acid T (5 mg/kg for i.v. or 14 mg/kg for p.o.), the main pharmacokinetic parameters by intravenous injection were: t1/2α (5.46±1.25) min; t1/2β:(227.18±11.40) min; CL: (1.09±0.16) mL/(kg·min); A UC0-12 h: (3939.13±311.14) μg.min/mL; AUC0-12h (4681.96±710.70)μg.min/mL and the absolute bioavailability was 41.98%±2.40%. This method is simple, sensitive, and accurate. It is suitable for pharmacokinetic studies of ganoderic acid in rats. 展开更多
关键词 ganoderic acid T PHARMACOKINETICS Rat plasma HPLC
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Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity 被引量:7
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作者 KANG Li-Hua ZHANG Guo-Wei +2 位作者 ZHANG Jun-Fang QIN Bai GUAN Huai-Jin 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2020年第12期934-940,共7页
A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract formation.Ganoderic acid A(GAA),an effective lanostane triterpene,is widely reported as an antioxidant.Th... A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract formation.Ganoderic acid A(GAA),an effective lanostane triterpene,is widely reported as an antioxidant.The aim of this study is to investigate the potential effects of GAA on cataract formation.After lens epithelial cells(LECs)were exposed to UVB radiation for different periods,cell viability,apoptosis-related protein levels,malondialdehyde(MDA)and superoxide dismutase(SOD)activities were monitored.We found that cell viability,the Bcl-2/Bax ratio and SOD activity were increased,while Cleaved caspase-3 levels and MDA activity were decreased compared with those in UVB-impaired LECs after GAA treated.Furthermore,GAA activated PI3 K/AKT in UVB-impaired LECs and effectively delayed the occurrence of lens opacity in vitro.In conclusion,these findings demonstrated that GAA exhibited protective functions in SRA01/04 cells and rat lenses against UVB-evoked impairment through elevating cell viability and antioxidant activity,inhibiting cell apoptosis,activating the PI3 K/AKT pathway and delaying lens opacity. 展开更多
关键词 ganoderic acid A CATARACT Lens epithelial cells Cell vitality APOPTOSIS
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Germplasm resources and secondary metabolism regulation in Reishi mushroom(Ganoderma lucidum) 被引量:2
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作者 Xinyu He Yiwen Chen +6 位作者 Zhenhao Li Ling Fang Haimin Chen Zongsuo Liang Ann Abozeid Zongqi Yang Dongfeng Yang 《Chinese Herbal Medicines》 CAS 2023年第3期376-382,共7页
Ganoderma lucidum is a valuable medical macrofungus with a myriad of diverse secondary metabolites,in which triterpenoids are the major constituents.This paper introduced the germplasm resources of genus Ganoderma fro... Ganoderma lucidum is a valuable medical macrofungus with a myriad of diverse secondary metabolites,in which triterpenoids are the major constituents.This paper introduced the germplasm resources of genus Ganoderma from textual research,its distribution and identification at the molecular level.Also we overviewed G.lucidum in the components,the biological activities and biosynthetic pathways of ganoderic acid,aiming to provide scientific evidence for the development and utilization of G.lucidum germplasm resources and the biosynthesis of ganoderic acid. 展开更多
关键词 chemical constituents Ganoderma lucidum(Leyss.ex Fr.)Karst. ganoderic acid germplasm resources pharmacological activities secondary metabolism regulation
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Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS 被引量:12
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作者 Mei-lin CUI Huan-yi YANG Guo-qing HE 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2015年第12期998-1010,共13页
As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were ... As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H20 and CO2, as well as cleavage of the D-ring. 展开更多
关键词 Ganoderma lucidum WORT Response surface methodology ganoderic acids HPLC-ESI-MS
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