Extraction of Astragalus Polysaccharides and Ganoderma lucidum Mycelia Polysaccharides and Their in Vitro Antioxidative Effects

[Objectives]To explore the extraction and in vitro antioxidant effects of astragalus polysaccharides(APS)and Ganoderma lucidum mycelia polysaccharides(GLMPS).[Methods]By studying the polysaccharides of the herbal medicinal material Astragalus membranaceus and the fungal medicinal material Ganoderma lucidum mycelia,two polysaccharides were mixed according to different proportions and concentrations by using the principle of traditional Chinese medicine compound combination.The effect of polysaccharides on the scavenging ability of hydroxyl radical system was determined by salicylic acid method.[Results]When the compound ratios of GLMPS and APS were 1∶1,1∶4,1∶5,4∶1,and 5∶1,the scavenging effect of compound polysaccharides was better than that of single-component polysaccharides,and with the increase of concentration,the scavenging effect increased.When the ratio of GLMPS and APS was 5∶1,the hydroxyl radical scavenging rate of the compound polysaccharide reached 59.77%,which was 18.72%higher than that of single GLMPS and 28.58%higher than that of single APS.The scavenging effect of compound polysaccharide is closely related to the compound ratio and concentration.[Conclusions]APS and GLMPS can obtain better hydroxyl radical scavenging ability than single-component polysaccharides through compounding in appropriate proportions.In addition,within a suitable concentration range,as the concentration increases,the scavenging ability also increases.

Phytochemical Contents and Antioxidant Properties of Ganoderma lucidum Polysaccharides in Relation to Growth Stage and Culture Substrate

[Objectives]To determine physicochemical characteristics and antioxidant activity of Ganoderma lucidum polysaccharides(GLP)in different growth stages and culture media.[Methods]Five polysaccharides(GLP1,GLP2,GLP3,GLPW and GLPB)were extracted and purified from the fruiting body of G.lucidum at three growth stages and culture substrates(wood culture and bag culture).The chemical components and antioxidant activity of the five polysaccharides were determined.[Results]GLP1 contained the highest content of neutral sugar(87.65%)and GLP3 had the highest uronic acid content.All the samples mainly comprised glucose,galactose,mannose,xylose and arabose with different ratios.Moreover,their antioxidant activities were investigated on the basis of DPPH radical,ABTS radical,SOD-like activity,and antihaemolytic activity.Results indicated in all three growth stages GLP2 had the greatest antioxidant properties.In addition,the antioxidant activity of GLPW was significantly higher than that of GLPB.[Conclusions]Overall,by comparison,G.lucidum in growth stage two may have potential health benefits,and wood culture may be a superior choice of artificial cultivation due to their abundance of active polysaccharides.

Studies on Active Polysaccharides from Ganoderma lucidum vith Immune Activity

从云头状灵芝[Ganoderma Lucidum(Leyss.ex Fr.)Karst.]中分离得到三种灵芝多糖BN3A，BN3B及BN3C，它们均表现明显免疫调节作用。从灵芝多糖BN3B及BN3C中各分离得到四个多糖均一体，对其中主要成分BN3C1，BN3C3，BN3B1及BN3B3进行了物理及化学研究，它们的平均分子量依次为1.6×10^4，2.5×10^4，3.5×10^4及4.0×10^4。经完全酸水解、红外光谱测定、过碘酸氧化、甲酸生成、Smith降解等证明，BN3B1及BN3C1均为β－（1→6)(1→3）甙键相连的葡聚糖。BN3B3为阿拉伯半乳聚糖。BN3C3为由葡萄糖和阿拉伯糖组成的肽多糖。它们均为β－（1→6）（1→3）甙键相连。

Related gene expressions in anti-keratinocyte aging induced by Ganoderma lucidum polysaccharides

Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR.

GPP(composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora

Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides(GLP)and Polyporus umbellatus polysaccharides(PUP)in a ratio of 3:1 named GPP enhances innate immune function in mice through enhancing the function of macrophage cells and activity of natural killer(NK)cells.Here in our research,we further investigated the effect of GPP on the diversity and composition of intestinal flora,and explored its effect on colitis model mice.The immunoregulatory verification experiments of GPP were conducted in both normal and DSS-induced mice model.Our research showed that GPP increased the diversity of intestinal microorganisms in mice with the extension of administration time.Daily GPP intake attenuated DSS-induced colon injury,protected the splenic lymphocyte proliferation ability,enhanced the serum hemolysin synthesis,and increased peripheral phagocytes and NK cell activity in model mice.Comparisons of the predominant gene pathways of the bacterial microbiota showed that DNA repair and recombination,base mismatch repair pathways was stronger in GPP-treatment group than in control group,indicating the possible molecular mechanisms of immune function regulation.Our study showed that GPP regulated immune function in both health and colitis model,and had a positive effect on maintaining intestinal flora homeostasis.

Optimization of enzyme assisted extraction of polysaccharides from Ganoderma lucidum

In the present work,an enzyme assisted extraction method is used to isolate crude polysaccharides from Ganoderma lucidum. The isolating effect was optimized with orthographic graph statistic method with three levels and four independent variables. Complex enzyme,extraction temperature,extraction time and extraction pH were combined to obtain the best possible combination to get maximum amount of extract and crude polysaccharides yield. The optimum extraction conditions were:complex enzyme amount of 3 %(w/v),extraction temperature at 45 ℃,extraction time of 3 h and extraction pH at 7. Under these conditions,the experimental amount of extract is 8.9 % and the yield of crude polysaccharides is 1.1 %,which are in close agreement with the value predicted by the model.

Effects of Ganoderma lucidum polysaccharides peptide on tumor metastasis in mice with sleep fragmentation

In recent years,studies have shown that there is a correlation between sleep disorders and cancer,but at the present stage,the research on sleep disorders and tumor related animal models is relatively insufficient.Our research will focus on mice bearing B16F10-luc-G5 melanoma tumor with sleep fragmentation,detecting promoting effect of sleep fragmentation(SF)on the metastasis of melanoma.At the same time,we used Ganoderma lucidum poly⁃saccharides peptide(GL-pp,80 mg·kg-1),a component of traditional Chinese medicine Ganoderma lucidum,which has long enjoyed a good reputation at home and abroad,to observe its anti-tumor metastasis effects on B16F10-luc-G5 mice with SF.Then we used whole proteomics to analyze the difference proteins expressed in lung tissue and compared between groups,includes mice bearing B16F10-luc-G5,mice bearing B16F10-luc-G5 with SF and GL-pp administered mice bearing B16F10-luc-G5 with SF.With the analysis using bioinformatics,we found several key proteins,their genes name are Adcy9,ptk2,Yap1 and Lpin2,Per1 and Tim.And several important clusters,they are,immune system,platelet aggres⁃sion,energy metabolism,cell cytoskeleton,cell adhesion and circadian rhythms.Moreover,we detected the TLR4 signal pathway and macrophage differentiation to reconfirm the results of proteomics and trying to elucidate the mechanism of SF on tumor growth and metastasis and the effects of GL-pp.

Comparison of the antitumor activity of polysaccharides extracted by boiling water and enzyme assistance from Ganoderma lucidum

Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum. In this work, polysaccharides were extracted from Ganoderma lucidum with boiling water method and enzyme assisted method. The human liver hepatocellular carcinoma cell line HepG2 was used to compare the an- titumor effect of the two kinds of extraction with 3-（4, 5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） test. Both of these two kinds of Ganoderma lucidum polysaccharides reduced cell viability of cancer cell HepG2 in a dose and time-dependent manner. At low concentrations, there was no significant difference in the effectiveness of L 1 and L2; while at concentrations over 0.8 ug/mL, the difference in the effectiveness of L2 in comparison to L1 became significant. At the concentrations of 3.2 ug/mL, the cancer cells were almost killed in 2 d.

Isolation and Structural Determination of a Glucan from the Spores of Ganoderma lucidum

A water soluble, (1 -->6)-branched, (1 -->4) linked D-glucan (LB-B1), [alpha](D)(21) = +174.2 degrees (c 0.87, H2O), was obtained from a hot-water extract of the sporoderm-broken spores of Ganoderma lucidun (Fr.) Karst by HPSEC, with 0.001 mol/L sodium hydroxide as the eluant, the molecular weight (Mw) of LB-B1 was estimated to be 9.3 x 10(3). From the results of total hydrolysis, methylation analysis, acetolysis and 1D, 2D-NMR experimentation, it was concluded that LB-B1 was composed of repeating units with the following structure: alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p) 1 down arrow 6 (-->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4-)-alpha -D-Glc(p)-(1 -->)(n)

Ganoderma Lucidum polysaccharide peptides protect kidney from renal ischemia reperfusion injury via counteracting oxidative stress

OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress.METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the procedure.In vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods.RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP treatment.The abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP treatment.More apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell injury.CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury.

Hydrodynamic Shear Stress Affects Cell Growth and Metabolite Production by Medicinal Mushroom Ganoderma lucidum

By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.

The Impact on Extraction Effect of Polysaccharide from Ganoderma lucidum by Different Pretreatment

[Objectives] The research aimed to study the impact on extraction effect of polysaccharide from Ganoderma lucidum( lingzhi) by different pretreatment methods. [Methods] The impacts on extraction of G. lucidum polysaccharide by soaking,microwave and air flow fine pulverization were contrasted,and the extraction effect of G. lucidum polysaccharide by combining the optimal pretreatment manner with hot water extraction,alcohol extraction,alkali extraction,ultrasonic binding enzyme extraction,and microwave extraction was compared. Finally,the property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. [Results] The optimal pretreatment method was air flow fine pulverization. Compared with traditional method-direct extraction( coarse grinding combining hot water extraction),crude yield changed little,while polysaccharide content and yield were improved by 114% and 104%. The best combination manner was air flow fine pulverization pretreatment combining with alkali extraction. Compared with traditional method,crude yield,polysaccharide content and yield were improved by 76%,78% and 215% respectively. The property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. It was found that the treatment method had little impact on the property of G. lucidum polysaccharide. [Conclusions]Air flow fine pulverization pretreatment could greatly improve extraction effect of G. lucidum polysaccharide,which mainly improved the content and yield of G. lucidum polysaccharide,and extraction was more complete,with less impact on the property of the extracted polysaccharide. It was speculated that air flow fine pulverization pretreatment mainly destroyed mechanical support wall membrane structure of G. lucidum fine powder,making that intracellular functional substances completely dissolved out of the cell,and the content would be studied in follow-up experiment.

Treatment Effect of Fermentation of Ganoderma lucidum to Immune and Antioxidant Functions in Immunosuppressed Mice

The immune regulatory and antioxidant roles of Ganoderma lucidum were investigated using cyclophosphamide(CTX)-induced immunosuppressed mice. Mice were randomly divided into groups: untreated(groupⅠ), immunosuppressed(groupⅡ), unfermented G. lucidum polysaccharide(groupⅢ) and fermented G. lucidum polysaccharide(group Ⅳ). After seven consecutive days of treatments, the serum concentration of IL-4, IFN-gamma, IgG, IgA and IgM and the liver activity of GSH-Px, SOD, CAT and MDA enzymes were analyzed. The contents of IL-4, IFN-γ in serum and GSH-Px, SOD and CAT in liver tissues were significantly reduced in groupⅡ compared with those in group I, indicating successful CTX-induced immunosuppression. Interestingly, the results showed that the above immune and antioxidant indicators were significantly improved after G. lucidum polysaccharide treatment, regardless of fermentation. However, fermentation caused changes in polysaccharide structure, which might have a significant impact on immune regulation and antioxidant functions in immunosuppressed mice.

Ganoderma lucidum polysaccharide peptide alleviates hepatoteatosis via modulating bile acid metabolism dependent on FXR-SHP/FGF

OBJECTIVE Ganoderma lucidum polysaccharide peptide(GLPP)is a group of extract from Ganoderma lucidum with a molecular mass of approximately 5×10^5,which ratio of polysaccharide to peptide is approximately 95%/5%.The purpose of this study was to determine whether GLPP has therapeutic effect on Non-alcoholic fatty liver disease(NAFLD).METHODS Ob/ob mouse model and ApoC3 transgenic mouse model were used for exploring the effect of GLPP on NAFLD.Key metabolic pathways and enzymes were identified by metabolomics combining with KEGG and PIUmet analyses and key enzymes were detected by Western blotting.Hepatosteatosis models of HepG2 cells and primary hepatocytes were used to further confirm the therapeutic effect of GLPP on NAFLD.RESULTS GLPP administrated for a month alleviated hepatosteatosis,dyslipidemia,liver dysfunction and liver insulin resistance.Pathways of glycerophos⁃pholipid metabolism,fatty acid metabolism and primary bile acid biosynthesis were involved in the therapeutic effect of GLPP on NAFLD.Detection of key enzymes revealed that GLPP reversed low expression of CYP7A1,CYP8B1,FXR,SHP and high expression of FGFR4 in ob/ob mice and ApoC3 mice.Besides,GLPP inhibited fatty acid synthesis by reducing the expression of SREBP1c,FAS and ACC via a FXR-SHP dependent mechanism.Additionally,GLPP reduced the accumulation of lipid droplets and the content of TG in HepG2 cells and primary hepatocytes induced by oleic acid and palmitic acid.CONCLUSION GLPP significantly improves NAFLD via regulating bile acid synthesis dependent on FXR-SHP/FGF pathway,which finally inhibits fatty acid synthesis,indicating that GLPP might be developed as a ther⁃apeutic drug for NAFLD.

Renal protective effect of Ganoderma lucidum

OBJECTIVE Non-alcoholic fatty liver disease(NAFLD) encompasses a series of patho.logic changes ranging from steatosis to steatohepatitis,which may progress to cirrhosis and hepatocel.lular carcinoma.The purpose of this study was to determine whether Ganoderma lucidum polysaccha.ride peptide(GLPP) has therapeutic effect on NAFLD.METHODS ob/ob mouse model and ApoC3 transgenic mouse model were used for exploring the effect of GLPP on NAFLD.Key metabolic path.ways and enzymes were identified by metabolomics combining with KEGG and PIUmet analyses and key enzymes were detected by Western blotting.Hepatosteatosis models of HepG2 cells and primary hepatocytes were used to further confirm the therapeutic effect of GLPP on NAFLD.RESULTS GLPP administrated for a month alleviated hepatosteatosis,dyslipidemia,liver dysfunction and liver insulin resistance.Pathways of glycerophospholipid metabolism,fatty acid metabolism and primary bile acid biosynthesis were involved in the therapeutic effect of GLPP on NAFLD.Detection of key enzymes revealed that GLPP reversed low expression of CYP7 A1,CYP8 B1,FXR,SHP and high expression of FGFR4 in ob/ob mice and ApoC3 mice.Besides,GLPP inhibited fatty acid synthesis by reducing the expression of SREBP1 c,FAS and ACC via a FXR-SHP dependent mechanism.Additionally,GLPP reduced the accumulation of lipid droplets and the content of TG in HepG2 cells and primary hepato.cytes induced by oleic acid and palmitic acid.CONCLUSION GLPP significantly improves NAFLD via regulating bile acid synthesis dependent on FXR-SHP/FGF pathway,which finally inhibits fatty acid synthesis,indicating that GLPP might be developed as a therapeutic drug for NAFLD.

Effect of Ganoderma lucidum Polysaccharide on Antioxidative Ability of Rats with Myocardial Injury Induced by Doxorubicin

Objective: The objective is to investigate the effect of Ganoderma lucidum polysaccharide (GLPS) on the oxidative stress induced by doxorubicin (DOX) in cardiomyocytes. Methods: SD rats were divided into control group, DOX group, GLPS low dose and high dose + DOX group. SOD and MDA levels in myocardial tissue were detected in each group. The expression of related proteins in each group was detected by Western blot. Results: GLPS can increase the SOD level and decrease MDA caused by DOX (p Conclusion: Ganoderma polysaccharide can improve the oxidative stress injury of myocardial tissue induced by doxorubicin and play a protective role in myocardial tissue.

破壁前后灵芝孢子粗多糖含量、结构及单糖组成对比分析

为明确破壁对灵芝孢子粗多糖含量、结构及单糖组成的影响,以灵芝粗多糖提取含量为指标,对灵芝粗多糖的提取工艺进行优化,获得灵芝孢子粗多糖超声辅助水提取的最佳工艺参数为超声温度55℃、超声时间8 h,此工艺条件下从破壁灵芝孢子粉中提取的粗多糖含量为(15.873±0.746)mg/g。采用最佳提取工艺条件提取破壁前后灵芝孢子粗多糖,对比其灵芝孢子粗多糖含量、纯度、DPPH自由基清除率以及单糖组成和多糖结构,结果显示破壁处理后灵芝孢子粗多糖含量提高233.396%,但其纯度下降了19.898%;破壁后灵芝孢子多糖DPPH自由基清除率也有较大提升;破壁处理对灵芝孢子多糖的单糖组成种类没有明显改变,但在单糖含量上存在显著性差异,特别是岩藻糖、半乳糖和甘露糖;红外光谱结果显示破壁前后灵芝孢子粉提取的灵芝孢子粗多糖在结构、组成上无明显差异,均是含有D⁃吡喃葡萄糖环的多糖。

灵芝多糖对糖尿病肾病小鼠的改善作用及机制研究

研究了灵芝多糖(Ganoderma lucidum polysaccharide,GLP)对糖尿病肾病(diabetic nephropathy,DN)小鼠的作用。成功构建DN小鼠模型后GLP干预。检测小鼠空腹血糖和24 h尿蛋白含量,测定尿素氮(blood urea nitrogen,BUN)和肌酐(serum creatinine,Scr)水平,结合伊红和马松染色观察肾脏病理形态,采用酶联免疫吸附试验测定肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、白细胞介素6(interleukin-6,IL-6)和白细胞介素10(interleukin-10,IL-10)的水平,使用蛋白免疫印迹法检测晚期糖基化终产品受体(receptor for advanced glycation end-products,RAGE)、Ⅳ型胶原蛋白(collagen type IV,COL-Ⅳ)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)蛋白的表达含量。DN组小鼠空腹血糖、24 h尿蛋白、BUN和Scr及其肾组织中TNF-α和IL-6水平均升高(P<0.05),血清中IL-10水平降低(P<0.05),RAGE、COL-Ⅳ和iNOS蛋白的表达量升高(P<0.05);而治疗组小鼠病理损伤及纤维化改变均有改善,定量指标得到好转。GLP可以改善DN小鼠,机制与抑制RAGE信号通路有关。

基于骨骼肌氧化应激作用探讨纳米硒提高小鼠运动耐力

以灵芝多糖(GLP)为分散剂,采用亚硒酸钠-抗坏血酸氧化还原法制备多糖纳米硒复合物(SeNPs),对SeNPs进行结构表征并探讨其对小鼠运动耐力的作用。结果表明:SeNPs颗粒大小67.25 nm,PDI为0.31,Zeta电位-66.72 mV;SeNPs和GLP红外光谱图高度相似,未发现新特征吸收峰,SeNPs重均分子量、数均分子量分别为7821.84 Da、6375.19 Da;SeNPs的最大热失重温度330℃,质量损失42.65%。与空白对照组相比,SeNPs低、中、高剂量组小鼠力竭游泳时间分别提高了12.69%、24.77%、37.84%;与运动模型组相比,SeNPs低、中、高剂量组小鼠血清肌酸激酶、乳酸脱氢酶活性和骨骼肌丙二醛含量均显著降低,抗氧化酶活性则显著升高,且上调了Nrf2和HO-1的蛋白表达。综上可知,SeNPs具有激活骨骼肌Nrf2信号通路,降低骨骼肌氧化应激水平的作用,进而提高小鼠运动耐力。

高多糖含量灵芝新品种‘沪农灵芝4号’选育

为选育高多糖含量灵芝(Ganoderma lucidum)新品种,以‘龙芝2号’(简称‘201’)为出发菌株,对其原生质体进行紫外诱变,获得806个再生菌株,从中筛选出153个与出发菌株具有拮抗反应,菌丝长势较好且稳定的突变株,并利用随机扩增多态性DNA(randomly amplified polymorphic DNA,RAPD)和简单重复序列(inter-simple sequence repeat,ISSR)分子标记进行聚类分析,结合菌株菌丝体的生物学特性,从聚类分组中选取具代表性的56个突变株进行出芝试验。基于子实体多糖含量、菌棒成品率、菌棒出芝率和子实体产量,从中筛选到综合指标最优的突变株L2-UV59。L2-UV59子实体产量0.103 kg·kg^(-1)干料,多糖含量2.24%,分别较出发菌株提高37.33%和34.13%。示范栽培结果表明,L2-UV59在大田栽培中性状稳定,具有良好的商业应用价值。L2-UV59已通过上海市非主要农作物品种认定,定名为‘沪农灵芝4号’。