Objective: To explore the effect of gardenia blue pigment on the anterior capsule staining and its safety performance. Methods: In this study, New Zealand white rabbits were used as the research object to test the eff...Objective: To explore the effect of gardenia blue pigment on the anterior capsule staining and its safety performance. Methods: In this study, New Zealand white rabbits were used as the research object to test the effect of gardenia blue pigment on the anterior capsule staining and anterior segment toxicity. In this study, gardenia blue stains of different concentrations (5%, 4%, 3%, 2%, 1%, 0.5%) were prepared to stain the anterior capsule of rabbit crystals, and the effect of the stain was observed to determine the optimal concentration of the stain. Twenty-seven healthy New Zealand white rabbits without eye disease were randomly divided into three groups: gardenia blue staining group;bio-blue positive drug group;physiological saline group. The intraocular pressure, anterior chamber inflammation, corneal endothelial injury, and anterior chamber angle pathological changes were measured in 1D, 7D, and 14D. Results: The results of this study showed that the effect of gardenia blue pigment on the anterior capsule of the lens was the best when the concentration of blue pigment was 2%. There was no change in intraocular pressure in the anterior chamber for a short period of time. There is no damage to the corneal endothelium. Conclusion: The results of this study show that the effect of gardenia blue pigment on the anterior capsule is best when the concentration of blue pigment is 2%, and the stain has no obvious toxic and side effects on the anterior segment of the eye.展开更多
Gardenia blue is a natural blue pigment that is environmentally friendly,non-toxic,and stable.The hydrolysis of geniposide,catalyzed byβ-glucosidase,is a critical step in the production process of gardenia blue.Howev...Gardenia blue is a natural blue pigment that is environmentally friendly,non-toxic,and stable.The hydrolysis of geniposide,catalyzed byβ-glucosidase,is a critical step in the production process of gardenia blue.However,β-glucosidase is not resistant to high temperatures,limiting the production of gardenia blue.In this study,we investigated the effectiveness of a heat-resistant glucosidase obtained from Thermotoga maritima in the production of gardenia blue.The enzyme exhibited a maximum activity of 10.60 U/mL at 90℃.Single-factor and orthogonal analyses showed that exogenously expressed heat-resistant glucosidase reacted with 470.3μg/mL geniposide and 13.5μg/mL glycine at 94.2℃,producing a maximum yield of 26.2857μg/mL of gardenia blue after 156.6 min.When applied to the dyeing of denim,gardenia blue produced by this method yielded excellent results;the best color-fastness was achieved when an iron ion mordant was used.This study revealed the feasibility and application potential of microbial production of gardenia blue.展开更多
基金Liaoning Natural Science Foundation:Research on functional dyes for intraocular biofilms(No.20180550004)Shenyang Science and Technology Plan Project:Preparation of Chitosan Bioactive Dressing and Study on Reconstruction of Bulbular Conjunctiva(No.19-112-4-069)Liaoning Provincial Natural Science Foundation:A study on the role of a degradable bioactive dressing in the repair of bulbar conjunctiva(No.2019-MS-257)
文摘Objective: To explore the effect of gardenia blue pigment on the anterior capsule staining and its safety performance. Methods: In this study, New Zealand white rabbits were used as the research object to test the effect of gardenia blue pigment on the anterior capsule staining and anterior segment toxicity. In this study, gardenia blue stains of different concentrations (5%, 4%, 3%, 2%, 1%, 0.5%) were prepared to stain the anterior capsule of rabbit crystals, and the effect of the stain was observed to determine the optimal concentration of the stain. Twenty-seven healthy New Zealand white rabbits without eye disease were randomly divided into three groups: gardenia blue staining group;bio-blue positive drug group;physiological saline group. The intraocular pressure, anterior chamber inflammation, corneal endothelial injury, and anterior chamber angle pathological changes were measured in 1D, 7D, and 14D. Results: The results of this study showed that the effect of gardenia blue pigment on the anterior capsule of the lens was the best when the concentration of blue pigment was 2%. There was no change in intraocular pressure in the anterior chamber for a short period of time. There is no damage to the corneal endothelium. Conclusion: The results of this study show that the effect of gardenia blue pigment on the anterior capsule is best when the concentration of blue pigment is 2%, and the stain has no obvious toxic and side effects on the anterior segment of the eye.
基金funded by Guangdong Provincial Undergraduate Training Program for Innovation and Entrepreneurship(grant No.202110590065)the Undergraduate Academic Competition Project of Shenzhen University(grant No.803-0000290846).
文摘Gardenia blue is a natural blue pigment that is environmentally friendly,non-toxic,and stable.The hydrolysis of geniposide,catalyzed byβ-glucosidase,is a critical step in the production process of gardenia blue.However,β-glucosidase is not resistant to high temperatures,limiting the production of gardenia blue.In this study,we investigated the effectiveness of a heat-resistant glucosidase obtained from Thermotoga maritima in the production of gardenia blue.The enzyme exhibited a maximum activity of 10.60 U/mL at 90℃.Single-factor and orthogonal analyses showed that exogenously expressed heat-resistant glucosidase reacted with 470.3μg/mL geniposide and 13.5μg/mL glycine at 94.2℃,producing a maximum yield of 26.2857μg/mL of gardenia blue after 156.6 min.When applied to the dyeing of denim,gardenia blue produced by this method yielded excellent results;the best color-fastness was achieved when an iron ion mordant was used.This study revealed the feasibility and application potential of microbial production of gardenia blue.
