OBJECTIVE To investigate expression of the tissue factor (TF)and matrix metalloproteinase-9(MMP-9)in breast cancers, and to assess their expression in relation to possible prognostic significance. METHODS The expressi...OBJECTIVE To investigate expression of the tissue factor (TF)and matrix metalloproteinase-9(MMP-9)in breast cancers, and to assess their expression in relation to possible prognostic significance. METHODS The expression of TF and MMP-9 in 71 breast cancer specimens were determined by EnVision immunohistochemistry, and the positive expressions related to the patient clinical outcome. RESULTS Positive rates of TF and MMP-9 staining were respectively 43.7%and 42.3%.K-M monofactorial analysis showed that the 5-year survival rate of the patients with a positive expression of TF and MMP-9 was lower than those with negative expression(P<0.05).However,the COX multifactorial analysis indicated that TNM staging and lymph node metastasis were the prognostic factors for breast cancer patients,and that TF and MMP-9 could not be used as the independent prognostic factors(P >0.05). CONCLUSION The positive rates of TF and MMP-9 were considerably high in breast cancers,which could provide useful information for patient prognosis.展开更多
AIM: To examine the effects of Helicobacter pylori(Hpylori) infection on the invasiveness of gastric cancer cells,and to elucidate its mechanism. METHODS: Gastric carcinoma cells, MKN-45, were incubated with CagA-posi...AIM: To examine the effects of Helicobacter pylori(Hpylori) infection on the invasiveness of gastric cancer cells,and to elucidate its mechanism. METHODS: Gastric carcinoma cells, MKN-45, were incubated with CagA-positive H pylori, and cell invasion was determined by Matrigel analysis.The expression of matrix metallopr-oteinase-9 (MMP-9),vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2) were assessed by Western-blot analysis, and transcriptional activation of the COX-2 promoter was examined by measuring luciferase and β-galactosidase activities. Lastly,the proteinDNA interaction was confirmed by an electrophoretic mobility shift assay. RESULTS: The current studies showed that: (1) incubation of CagA-positive H pylori with MKN-45 cells significantly promotes gastric cancer cells invasion, and this effect is attenuated by pre-treatment with NS-398, a COX-2 inhibitor, or PDTC,a nuclear factor κB (NF-κB) inhibitor;(2) the induction of MKN-45 cells invasion by Hpylori is associated with increases in COX-2, MMP-9, and VEGF protein expression, and co-incubation of NS-398 or PDTC significantly reduces these effects;(3) H pylori infection transactivates COX-2 promoter activity and increases the binding of NF-κB to this promoter. CONCLUSION: Our data demonstrate that H pylori infection promotes gastric epithelial cells invasion by activating MMP-9 and VEGF expression. These effects appear to be mediated through a NF-κB and COX-2 mediated pathway, as COX-2 or NF-κB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein.展开更多
Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: ...Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.展开更多
文摘OBJECTIVE To investigate expression of the tissue factor (TF)and matrix metalloproteinase-9(MMP-9)in breast cancers, and to assess their expression in relation to possible prognostic significance. METHODS The expression of TF and MMP-9 in 71 breast cancer specimens were determined by EnVision immunohistochemistry, and the positive expressions related to the patient clinical outcome. RESULTS Positive rates of TF and MMP-9 staining were respectively 43.7%and 42.3%.K-M monofactorial analysis showed that the 5-year survival rate of the patients with a positive expression of TF and MMP-9 was lower than those with negative expression(P<0.05).However,the COX multifactorial analysis indicated that TNM staging and lymph node metastasis were the prognostic factors for breast cancer patients,and that TF and MMP-9 could not be used as the independent prognostic factors(P >0.05). CONCLUSION The positive rates of TF and MMP-9 were considerably high in breast cancers,which could provide useful information for patient prognosis.
基金Supported by the Taichung Veterans General Hospital Research Grant: TCVGH-933308C
文摘AIM: To examine the effects of Helicobacter pylori(Hpylori) infection on the invasiveness of gastric cancer cells,and to elucidate its mechanism. METHODS: Gastric carcinoma cells, MKN-45, were incubated with CagA-positive H pylori, and cell invasion was determined by Matrigel analysis.The expression of matrix metallopr-oteinase-9 (MMP-9),vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2) were assessed by Western-blot analysis, and transcriptional activation of the COX-2 promoter was examined by measuring luciferase and β-galactosidase activities. Lastly,the proteinDNA interaction was confirmed by an electrophoretic mobility shift assay. RESULTS: The current studies showed that: (1) incubation of CagA-positive H pylori with MKN-45 cells significantly promotes gastric cancer cells invasion, and this effect is attenuated by pre-treatment with NS-398, a COX-2 inhibitor, or PDTC,a nuclear factor κB (NF-κB) inhibitor;(2) the induction of MKN-45 cells invasion by Hpylori is associated with increases in COX-2, MMP-9, and VEGF protein expression, and co-incubation of NS-398 or PDTC significantly reduces these effects;(3) H pylori infection transactivates COX-2 promoter activity and increases the binding of NF-κB to this promoter. CONCLUSION: Our data demonstrate that H pylori infection promotes gastric epithelial cells invasion by activating MMP-9 and VEGF expression. These effects appear to be mediated through a NF-κB and COX-2 mediated pathway, as COX-2 or NF-κB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein.
文摘Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.
