AIM： To analyze expression of ATP7B in gastric cardiac adenocarcinomas, its clinicopathologic significance, in comparison with distal gastric adenocarcinomas. METHODS： Immunohistochemical avidin-biotin peroxidase co...AIM： To analyze expression of ATP7B in gastric cardiac adenocarcinomas, its clinicopathologic significance, in comparison with distal gastric adenocarcinomas. METHODS： Immunohistochemical avidin-biotin peroxidase complex method was applied to detect the expression of ATP7B in 49 cases of cardiac carcinomas, the corresponding adjacent non-neoplastic epithelium and 55 cases of distal gastric carcinomas. RESULTS： The proportion of ATP7B positive samples in gastric cardiac carcinomas （51.0%, 25 of 49） was significantly higher than that in the corresponding adjacent non-neoplastic epithelium （22.4%, 11 of 49） （P = 0.003）. ATP7B expression in poorly differentiated gastric cardiac carcinomas was significantly higher than that in well/moderately differentiated gastric cardiac carcinomas （P = 0.030）. ATP7B expression in gastric cardiac carcinomas was independent of age, tumor size, nodal stage and metastasis status. ATP7B protein was detected in 30.9% （17/55 cases） of distal gastric carcinomas, markedly lower than that in gastric cardiac carcinomas （P = 0.037）. CONCLUSION： ATP7B protein is frequently overexpressecl in gastric cardiac carcinomas, and correlated with the differentiation of cardiac carcinoma. ATP7B expression in gastric cardiac carcinomas is significantly higher than that in distal gastric carcinomas, which might partially explain the difference of chemotherapy response and prognosis between these two gastric carcinomas.展开更多
Background We investigated the possible association of the functional polymorphisms in the tumor necrosis factor （TNF） genes with susceptibility to esophageal squamous cell carcinoma （ESCC） and gastric cardiac ade...Background We investigated the possible association of the functional polymorphisms in the tumor necrosis factor （TNF） genes with susceptibility to esophageal squamous cell carcinoma （ESCC） and gastric cardiac adenocarcinoma （GCA）.Methods The TNF-α-308G/A and TNF-β ＋ 252G/A single nucleotide polymorphisms （SNPs） were genotyped using polymerase-chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis, in 555 cancer patients （291 ESCC and 264 GCA） and 437 healthy controls in a high incidence region of North China. Results Among healthy controls, frequencies of the TNF-α 1/1, 1/2 and 2/2 genotypes were 89.4% ,9.2% and 1.4% respectively, while frequencies of the TNF-β B1/B1, B1/B2 and B2/B2 genotypes were 12.6% , 32.3% and 55.1%, respectively. No significant difference was found in the overall genotype and allelotype distribution of the TNF-α-308G/A and TNF-β ＋ 252G/A SNPs among cancer patients and controls. However, both the B1/B1 genotype and B1/B2 genotype significantly increased the risk of developing ESCC [ the age and gender adjusted odds ratio （OR） =2.04 and 1.91, 95% confidence interval （CI） = 1.04 -4.43 and 1.14 - 2.60, respectively] and GCA （the age and gender adjusted OR =2. 68 and 2. 64, 95% CI = 1.14 -6.29 and 1.47 -4.72, respectively） in individuals with negative family history of UGIC, in comparison with the B2/B2 genotype. When the two TNF polymorphisms were combined and analyzed, individuals with the TNF-β B1/B2 and TNF-α1/2 or 2/2 genotypes significantly reduced the risk of developing ESCC and GCA, in comparison with those harboring the TNF-β B2/B2 and TNF-α 1/1 genotypes （ the age and gender adjusted OR = 0.37 and 0. 34, 95% CI =0. 15 -0.92 and 0. 13 -0.90, respectively）. Conclusions Therefore, the TNF-α -308G/A and TNF-β ＋ 252G/A genotyping may be used as a stratification markers to predicate the risk of ESCC and GCA development in North China.展开更多
目的:比较完全3D腹腔镜胃底贲门癌根治术(completely 3D laparoscopic radical resection of the gastric cardia carcinoma,C3DLRRGCC)与开放手术(radical resection of the gastric cardia carcinoma,RRGCC)的优势与不足,探讨完全腹...目的:比较完全3D腹腔镜胃底贲门癌根治术(completely 3D laparoscopic radical resection of the gastric cardia carcinoma,C3DLRRGCC)与开放手术(radical resection of the gastric cardia carcinoma,RRGCC)的优势与不足,探讨完全腹腔镜胃底贲门癌根治术的可行性。方法:选取2012年1月至2014年12月行C3DLRRGCC的96例患者,其中76例行根治性全胃切除术,20例行根治性近端胃切除术,并与108例RRGCC的患者进行回顾性分析,比较其可行性。结果:96例C3DLRRGCC病例均行标准D2淋巴结清扫术。全胃切除术时,开腹组手术时间优于腹腔镜组(P<0.05),腹腔镜组术中出血量、清扫淋巴结数量、术后胃肠道恢复时间、术后切口感染例数、随访期内肠梗阻例数优于开腹组(P<0.05),两组患者术后出血例数、吻合口漏发生率差异无统计学意义(P>0.05)。近端胃切除时,腹腔镜组术中出血量、胃肠道恢复时间优于开腹组(P<0.05)。两组均无术后住院期间死亡病例。结论:C3DLRRGCC是安全、可行的,能达到开腹手术的临床效果与清扫目的。展开更多
文摘AIM： To analyze expression of ATP7B in gastric cardiac adenocarcinomas, its clinicopathologic significance, in comparison with distal gastric adenocarcinomas. METHODS： Immunohistochemical avidin-biotin peroxidase complex method was applied to detect the expression of ATP7B in 49 cases of cardiac carcinomas, the corresponding adjacent non-neoplastic epithelium and 55 cases of distal gastric carcinomas. RESULTS： The proportion of ATP7B positive samples in gastric cardiac carcinomas （51.0%, 25 of 49） was significantly higher than that in the corresponding adjacent non-neoplastic epithelium （22.4%, 11 of 49） （P = 0.003）. ATP7B expression in poorly differentiated gastric cardiac carcinomas was significantly higher than that in well/moderately differentiated gastric cardiac carcinomas （P = 0.030）. ATP7B expression in gastric cardiac carcinomas was independent of age, tumor size, nodal stage and metastasis status. ATP7B protein was detected in 30.9% （17/55 cases） of distal gastric carcinomas, markedly lower than that in gastric cardiac carcinomas （P = 0.037）. CONCLUSION： ATP7B protein is frequently overexpressecl in gastric cardiac carcinomas, and correlated with the differentiation of cardiac carcinoma. ATP7B expression in gastric cardiac carcinomas is significantly higher than that in distal gastric carcinomas, which might partially explain the difference of chemotherapy response and prognosis between these two gastric carcinomas.
基金This study was supported by a grant from the National NaturalScience Foundation China (No.30371591)
文摘Background We investigated the possible association of the functional polymorphisms in the tumor necrosis factor （TNF） genes with susceptibility to esophageal squamous cell carcinoma （ESCC） and gastric cardiac adenocarcinoma （GCA）.Methods The TNF-α-308G/A and TNF-β ＋ 252G/A single nucleotide polymorphisms （SNPs） were genotyped using polymerase-chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis, in 555 cancer patients （291 ESCC and 264 GCA） and 437 healthy controls in a high incidence region of North China. Results Among healthy controls, frequencies of the TNF-α 1/1, 1/2 and 2/2 genotypes were 89.4% ,9.2% and 1.4% respectively, while frequencies of the TNF-β B1/B1, B1/B2 and B2/B2 genotypes were 12.6% , 32.3% and 55.1%, respectively. No significant difference was found in the overall genotype and allelotype distribution of the TNF-α-308G/A and TNF-β ＋ 252G/A SNPs among cancer patients and controls. However, both the B1/B1 genotype and B1/B2 genotype significantly increased the risk of developing ESCC [ the age and gender adjusted odds ratio （OR） =2.04 and 1.91, 95% confidence interval （CI） = 1.04 -4.43 and 1.14 - 2.60, respectively] and GCA （the age and gender adjusted OR =2. 68 and 2. 64, 95% CI = 1.14 -6.29 and 1.47 -4.72, respectively） in individuals with negative family history of UGIC, in comparison with the B2/B2 genotype. When the two TNF polymorphisms were combined and analyzed, individuals with the TNF-β B1/B2 and TNF-α1/2 or 2/2 genotypes significantly reduced the risk of developing ESCC and GCA, in comparison with those harboring the TNF-β B2/B2 and TNF-α 1/1 genotypes （ the age and gender adjusted OR = 0.37 and 0. 34, 95% CI =0. 15 -0.92 and 0. 13 -0.90, respectively）. Conclusions Therefore, the TNF-α -308G/A and TNF-β ＋ 252G/A genotyping may be used as a stratification markers to predicate the risk of ESCC and GCA development in North China.
文摘目的:比较完全3D腹腔镜胃底贲门癌根治术(completely 3D laparoscopic radical resection of the gastric cardia carcinoma,C3DLRRGCC)与开放手术(radical resection of the gastric cardia carcinoma,RRGCC)的优势与不足,探讨完全腹腔镜胃底贲门癌根治术的可行性。方法:选取2012年1月至2014年12月行C3DLRRGCC的96例患者,其中76例行根治性全胃切除术,20例行根治性近端胃切除术,并与108例RRGCC的患者进行回顾性分析,比较其可行性。结果:96例C3DLRRGCC病例均行标准D2淋巴结清扫术。全胃切除术时,开腹组手术时间优于腹腔镜组(P<0.05),腹腔镜组术中出血量、清扫淋巴结数量、术后胃肠道恢复时间、术后切口感染例数、随访期内肠梗阻例数优于开腹组(P<0.05),两组患者术后出血例数、吻合口漏发生率差异无统计学意义(P>0.05)。近端胃切除时,腹腔镜组术中出血量、胃肠道恢复时间优于开腹组(P<0.05)。两组均无术后住院期间死亡病例。结论:C3DLRRGCC是安全、可行的,能达到开腹手术的临床效果与清扫目的。
