[Objective] This study aimed to investigate the four Gastrodia elata B1. variants at the DNA level. [Method] Primers with good reproducibility, abundant polymorphism and high resolution were selected from the 35 rando...[Objective] This study aimed to investigate the four Gastrodia elata B1. variants at the DNA level. [Method] Primers with good reproducibility, abundant polymorphism and high resolution were selected from the 35 random primers with RAPD marker technology and the amplification results were analyzed. [ Result] By using RAPD technology, DNA fingerprints of four Gastrodia elata B1. variants with abundant polymorphism, good reproducibility and high resolution was successfully constructed; to be specific, each Gastrodia elata B1. variant has its unique fingerprint with primers S29 and S38 ; DNA bands of the Gastrodia elata BI. variants vary significantly, which is contributive to distinguish the four Gastrodia clara B1. variants. [Condusion] This study provided an effective means for the identification and protection of Gastrodia elata germplasms with intraspecific variations.展开更多
[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research tri...[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research trial was carried out in the following three kinds of habitats at the same time: the plastic greenhouses, the birch forest of shady slope and the shrubbery of sunny slope, and the results were analyzed with group data average hypothesis test method. [Result] The overall tuber(the juvenile tuber, the immature tuber and the mature tuber) yield and the mature tuber yield were both very significantly higher in the plastic greenhouses than in the other two types of habitats, and respectively reached 9.52 and 6.70 kg/m2; the mature tuber drying rate was dramatically or very dramatically lower in the plastic greenhouses than in the other two types of habitats, and was 23.84%; the stabilities of the overall tuber yield, the mature tuber yield and the drying rate were all reduced in the order of greenhouses, birch forest and shrubbery; and the dry mature tuber yield of the plastic greenhouses was the highest in the three kinds of habitats, and was 1.60 kg/m2. [Conclusion] A good habitat had to be created or chosen in the domesticating cultivation of the wild Geg in Ganzi prefecture. The preferred choice was the plastic greenhouse, the alternative one was the deciduous broadleaved forest of shady slope. The imitating wild planting of the wild Geg should be done in the warm moist deciduous broad-leaved forest of shady slope.展开更多
The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tre...The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.展开更多
研究638株德江红天麻(Gastrodia elata BI. f. elata)的18个农艺性状,并进行变异系数、相关性、因子和通径分析。结果表明,德江红天麻地上部分农艺性状中,花茎粗度、花被筒长度、花被筒宽度、子房长度、果实长度和果实宽度具有较高的一...研究638株德江红天麻(Gastrodia elata BI. f. elata)的18个农艺性状,并进行变异系数、相关性、因子和通径分析。结果表明,德江红天麻地上部分农艺性状中,花茎粗度、花被筒长度、花被筒宽度、子房长度、果实长度和果实宽度具有较高的一致性和特异性,其因子贡献率和通径分析的正向直接作用也较大;地下部分主要农艺性状中,纵长、横长和环纹数的变异系数较小,与单个块茎鲜重有极为显著的相关性,其因子贡献率和通径分析的正向直接作用也较大。上述主要农艺性状可作为德江红天麻品质控制和品种选育的关键性状指标。展开更多
基金Supported by National Scientific and Technological Key Project of China(Grant No. 2004BA721A33)Scientific and Technological Key Project of Guizhou Province (Grant No.[2001]1125)Special Project of Research and Development of Traditional Chinese Medicine Modern Science and Technology Industry in Guizhou Province(Grant No.[2003]51)
文摘[Objective] This study aimed to investigate the four Gastrodia elata B1. variants at the DNA level. [Method] Primers with good reproducibility, abundant polymorphism and high resolution were selected from the 35 random primers with RAPD marker technology and the amplification results were analyzed. [ Result] By using RAPD technology, DNA fingerprints of four Gastrodia elata B1. variants with abundant polymorphism, good reproducibility and high resolution was successfully constructed; to be specific, each Gastrodia elata B1. variant has its unique fingerprint with primers S29 and S38 ; DNA bands of the Gastrodia elata BI. variants vary significantly, which is contributive to distinguish the four Gastrodia clara B1. variants. [Condusion] This study provided an effective means for the identification and protection of Gastrodia elata germplasms with intraspecific variations.
基金Supported by Key Natural Science Program of the Education Department of Sichuan province(12ZA103)
文摘[Objective] The influences of different habitats on asexual propagation of wild Geg(abbreviation for Gastrodia elata Bl. f. glauca S. Chow) being domesticated in Ganzi prefecture was studied. [Method] The research trial was carried out in the following three kinds of habitats at the same time: the plastic greenhouses, the birch forest of shady slope and the shrubbery of sunny slope, and the results were analyzed with group data average hypothesis test method. [Result] The overall tuber(the juvenile tuber, the immature tuber and the mature tuber) yield and the mature tuber yield were both very significantly higher in the plastic greenhouses than in the other two types of habitats, and respectively reached 9.52 and 6.70 kg/m2; the mature tuber drying rate was dramatically or very dramatically lower in the plastic greenhouses than in the other two types of habitats, and was 23.84%; the stabilities of the overall tuber yield, the mature tuber yield and the drying rate were all reduced in the order of greenhouses, birch forest and shrubbery; and the dry mature tuber yield of the plastic greenhouses was the highest in the three kinds of habitats, and was 1.60 kg/m2. [Conclusion] A good habitat had to be created or chosen in the domesticating cultivation of the wild Geg in Ganzi prefecture. The preferred choice was the plastic greenhouse, the alternative one was the deciduous broadleaved forest of shady slope. The imitating wild planting of the wild Geg should be done in the warm moist deciduous broad-leaved forest of shady slope.
基金Supported by Key Project of Natural Sciences of Sichuan Provincial Department of Education(12ZA103)
文摘The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.
文摘研究638株德江红天麻(Gastrodia elata BI. f. elata)的18个农艺性状,并进行变异系数、相关性、因子和通径分析。结果表明,德江红天麻地上部分农艺性状中,花茎粗度、花被筒长度、花被筒宽度、子房长度、果实长度和果实宽度具有较高的一致性和特异性,其因子贡献率和通径分析的正向直接作用也较大;地下部分主要农艺性状中,纵长、横长和环纹数的变异系数较小,与单个块茎鲜重有极为显著的相关性,其因子贡献率和通径分析的正向直接作用也较大。上述主要农艺性状可作为德江红天麻品质控制和品种选育的关键性状指标。