AIM： To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS： Gateway recombinant cloning technology was used to co...AIM： To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS： Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type （wt） and mutant （mut） forms of the lumican gene were synthesized and amplified by polymerase chain reaction （PCR）. The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown- multiple cloning site （MCS）-/internal ribozyme entry site （IRES）/enhanced green fluorescent protein （EGFP）. Then the desired DNA fragments were integrated into the destination vector pAV.Desld yielding the final expression constructs pAV.Exld-CMV〉wt-lumican/IRES/ EGFP and pAV.Exld-cytomegalovirus （CMV） 〉mutlumican/IRES/EGFP, respectively.RESULTS： The adenovirus plasmids pAV.Exld-CMV〉 wt-lumican/IRES/EGFP and pAV.Exld-CMV 〉mutlumican/IRESlEGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION： We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumicangene in the pathogenesis of high myopia.展开更多
Facing keen competition in telecom marketsworldwide, global mobile operators arelooking for the best solution that can notonly make full use of existing networkresources, but also introduce new technologiesfor new ser...Facing keen competition in telecom marketsworldwide, global mobile operators arelooking for the best solution that can notonly make full use of existing networkresources, but also introduce new technologiesfor new service provision.The CDMA network features great capacity,strong anti-jamming functionality, good voicequality and low power consumption of terminals,with which CDMA operators can reduce theirinvestment in equipment and cost of networkoperation and maintenance. In addition, whentransiting to 3G, the CDMA system canimplement smooth evolution from IS-95A toCDMA2000 1X and then to CDMA2000 1xEV withlow cost.展开更多
基金Supported by the Natural Science Foundation of Guangdong Province(No.2015A030310158No.2014A030313359)+1 种基金the Fundamental Research Funds for the Central Universities(No.21611446)the Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University(No.2015201)
文摘AIM： To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS： Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type （wt） and mutant （mut） forms of the lumican gene were synthesized and amplified by polymerase chain reaction （PCR）. The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown- multiple cloning site （MCS）-/internal ribozyme entry site （IRES）/enhanced green fluorescent protein （EGFP）. Then the desired DNA fragments were integrated into the destination vector pAV.Desld yielding the final expression constructs pAV.Exld-CMV〉wt-lumican/IRES/ EGFP and pAV.Exld-cytomegalovirus （CMV） 〉mutlumican/IRES/EGFP, respectively.RESULTS： The adenovirus plasmids pAV.Exld-CMV〉 wt-lumican/IRES/EGFP and pAV.Exld-CMV 〉mutlumican/IRESlEGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION： We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumicangene in the pathogenesis of high myopia.
文摘Facing keen competition in telecom marketsworldwide, global mobile operators arelooking for the best solution that can notonly make full use of existing networkresources, but also introduce new technologiesfor new service provision.The CDMA network features great capacity,strong anti-jamming functionality, good voicequality and low power consumption of terminals,with which CDMA operators can reduce theirinvestment in equipment and cost of networkoperation and maintenance. In addition, whentransiting to 3G, the CDMA system canimplement smooth evolution from IS-95A toCDMA2000 1X and then to CDMA2000 1xEV withlow cost.
