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Gateways to Investment
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作者 Sudeshna Sarkar 《ChinAfrica》 2015年第8期42-43,共2页
Opportunities exist for using Mauritius as a platform for investing in Africa. Not only [has] the quantity of Chinese foreign direct investment into Africa increased, but also the quality.
关键词 gateways to Investment
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Gateways Placement in Backbone Wireless Mesh Networks 被引量:2
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作者 Maolin TANG 《International Journal of Communications, Network and System Sciences》 2009年第1期44-50,共7页
This paper presents a novel algorithm for the gateway placement problem in Backbone Wireless Mesh Networks (BWMNs). Different from existing algorithms, the new algorithm incrementally identifies gateways and assigns m... This paper presents a novel algorithm for the gateway placement problem in Backbone Wireless Mesh Networks (BWMNs). Different from existing algorithms, the new algorithm incrementally identifies gateways and assigns mesh routers to identified gateways. The new algorithm can guarantee to find a feasible gateway placement satisfying Quality-of-Service (QoS) constraints, including delay constraint, relay load constraint and gateway capacity constraint. Experimental results show that its performance is as good as that of the best of existing algorithms for the gateway placement problem. But, the new algorithm can be used for BWMNs that do not form one connected component, and it is easy to implement and use. 展开更多
关键词 GATEWAY PLACEMENT BACKBONE WIRELESS MESH Networks
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矢车菊不同颜色花瓣酵母cDNA文库的构建
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作者 邓成燕 王佳颖 戴思兰 《北京林业大学学报》 CAS CSCD 北大核心 2024年第3期115-122,共8页
【目的】矢车菊花瓣的蓝色呈色和品种间花色变异的分子调控机制尚不明晰。本研究采用Gateway技术构建了矢车菊6个不同花色品种花瓣的酵母cDNA文库,以期进一步通过酵母单杂交或双杂交技术筛选参与调控花瓣呈色的关键互作蛋白。【方法】... 【目的】矢车菊花瓣的蓝色呈色和品种间花色变异的分子调控机制尚不明晰。本研究采用Gateway技术构建了矢车菊6个不同花色品种花瓣的酵母cDNA文库,以期进一步通过酵母单杂交或双杂交技术筛选参与调控花瓣呈色的关键互作蛋白。【方法】本研究以白色、粉色、红色、蓝色、紫色和墨色矢车菊花瓣为材料,提取总RNA后分离和纯化mRNA,合成双链cDNA后依次进行BP重组反应和LR重组反应,分别获得初级和次级文库。最后将次级文库质粒转化酵母Y187,获得矢车菊不同颜色花瓣的酵母cDNA文库。【结果】质量鉴定结果显示:初级文库的库容量为1.3×10^(7) CFU,重组率为100%,且插入片段长度均在1000 bp以上;次级文库的库容量为1.6×10^(7) CFU,重组率为100%,且插入片段长度均在1000 bp以上。酵母文库的滴度为3.5×10^(7) CFU/mL,随机挑选的24个单克隆经PCR检测后均扩增出明亮条带,重组率为100%,插入片段长度均大于1000 bp。【结论】本研究构建的矢车菊不同颜色花瓣酵母cDNA文库的质量较高,能满足酵母文库筛选的试验要求,为后续探究矢车菊花瓣的蓝色呈色和品种间花色变异的分子调控机制提供了材料基础。 展开更多
关键词 矢车菊 花色 酵母 CDNA文库 GATEWAY技术
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Phosphodiesterase 9 localization in cytoplasm and nucleus:the gateway to selective targeting in neuroprotection?
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作者 Giovanni Ribaudo Matteo Giannangeli +1 位作者 Margrate Anyanwu Alessandra Gianoncelli 《Neural Regeneration Research》 SCIE CAS 2025年第6期1701-1702,共2页
The umbrella term"neurodege ne rative disorders"(NDDs) refers to several conditions characterized by a progressive loss of structure and function of cells belonging to the nervous system.Such diseases affect... The umbrella term"neurodege ne rative disorders"(NDDs) refers to several conditions characterized by a progressive loss of structure and function of cells belonging to the nervous system.Such diseases affect more than 50million people worldwide.Neurodegenerative disorders are characterized by sundry factors and pathophysiological mechanisms that a re challenging to be fully profiled.Many of these rely on cell signaling pathways to preserve homeostasis,involving second messengers such as cyclic adenosine monophosphate (cAMP)and cyclic guanosine 3',5'-monophosphate(cGMP).Their ability to control the duration and amplitude of the signaling cascade is given by the presence of several common and uncommon effectors.Protein kinases A and G (PKA and PKG),phosphodiesterases (PDEs),and scaffold proteins are among them. 展开更多
关键词 GATEWAY HOMEOSTASIS preserve
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Development of Guangxi' s Tourism Economy During Chinese New Year
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作者 Liu Xiongsheng Xu Xiaoming 《China & The World Cultural Exchange》 2024年第4期30-33,共4页
Through a variety of folk customs and festivals,Guangxi makes full use of the national Intangible Cultural Heritage(ICH)to develop local folk tourism.Bordered by the Beibu Gulf in the south and the Southwest in the we... Through a variety of folk customs and festivals,Guangxi makes full use of the national Intangible Cultural Heritage(ICH)to develop local folk tourism.Bordered by the Beibu Gulf in the south and the Southwest in the west,Guangxi is an important gateway for China's opening up to the outside world.As the only ethnic minority autonomous region with advantages along the coast,along the river and along the border. 展开更多
关键词 OUTSIDE AUTONOMOUS GATEWAY
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Ancient Zhenyuan, Gateway to East Guizhou
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作者 MENG JIAXIN 《China Today》 2024年第1期76-80,共5页
This ancient city has a history going back over 2,000 years.It has witnessed the fusion of multiple ethnic cultures including the Miao,Dong,and Han,and hosts a number of cultural heritage sites.THE old town of Zhenyua... This ancient city has a history going back over 2,000 years.It has witnessed the fusion of multiple ethnic cultures including the Miao,Dong,and Han,and hosts a number of cultural heritage sites.THE old town of Zhenyuan is located in Zhenyuan County,Qiandongnan Miao and Dong Autonomous Prefecture in southwest China’s Guizhou Province.Covering an area of just around three square kilometers,it sits on the banks of the Wuyang River and is surrounded by mountains.Zhenyuan has been a county for more than 2,281 years and was the prefectural capital during the Yuan(1271-1368)and Qing(1644-1911)dynasties.It is a multicultural region,with Miao,Dong,and other ethnic minorities making up nearly half of the population. 展开更多
关键词 GUIZHOU GATEWAY SOUTHWEST
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芍药种子休眠解除过程的酵母杂交cDNA文库构建 被引量:1
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作者 费日雯 樊富伟 +4 位作者 孙天祎 辛如洁 宋文慧 关士鑫 孙晓梅 《北京林业大学学报》 CAS CSCD 北大核心 2023年第7期120-129,共10页
【目的】芍药具有极佳的观赏价值,但其种子的双重休眠难以解除,严重阻碍芍药的育种进程。本研究利用gateway技术构建芍药种子休眠解除关键时期的酵母杂交cDNA文库,可用于筛选调控芍药种子休眠解除相关基因的转录因子及互作蛋白。本文库... 【目的】芍药具有极佳的观赏价值,但其种子的双重休眠难以解除,严重阻碍芍药的育种进程。本研究利用gateway技术构建芍药种子休眠解除关键时期的酵母杂交cDNA文库,可用于筛选调控芍药种子休眠解除相关基因的转录因子及互作蛋白。本文库的构建可为丰富芍药种子休眠调控研究提供科学支撑,能为芍药栽培育种和野生资源保护及利用奠定分子理论基础。【方法】本研究以休眠解除过程中5个关键时期的芍药种子为试验材料,进行总RNA的提取和m RNA的分离,使用gateway方法构建cDNA初级文库,再通过LR重组,将初级文库重组到pGADT7-DEST次级文库载体上,构建出次级文库,最后经过酵母转化试验,将次级文库质粒转化到酵母Y187感受态细胞中,构建出芍药种子休眠解除过程的酵母文库。【结果】经鉴定,cDNA初级文库库容量为1.28×10^(7) CFU,平均插入片段长度在1000 bp以上,重组率为100%;cDNA次级文库库容量为1.12×10^(7) CFU,平均插入片段长度在1000 bp以上,重组率为100%;酵母文库滴度为7.0×10^(7) CFU/mL,平均插入片段长度在1000 bp以上,重组率为96%。23个阳性克隆测序结果经NCBI比对后,按照功能将20个已知蛋白序列划分为8类,其中7个序列已被报道参与种子的休眠与萌发。【结论】芍药种子休眠解除过程的酵母杂交cDNA文库构建的质量较高,具有较完整的基因信息,符合酵母文库筛选试验所需的标准,为构建芍药种子休眠解除过程的分子调控网络提供基础。 展开更多
关键词 芍药 种子休眠与萌发 酵母杂交 CDNA文库 GATEWAY技术
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多机制优化的模拟试验台数据流实时迁移研究
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作者 周启凡 郭迎清 +3 位作者 赵万里 王昆 孙浩 陶欣昕 《测控技术》 2023年第10期52-59,73,共9页
航空发动机高空模拟试验作为检验航空发动机性能的重要手段,其试验流程中采集的各传感器测点数据极为重要与珍贵。为了使数据信息的展示更为直观并且更深入挖掘数据潜在的价值,提出基于多框架集成的航空发动机高空模拟试验台数据流实时... 航空发动机高空模拟试验作为检验航空发动机性能的重要手段,其试验流程中采集的各传感器测点数据极为重要与珍贵。为了使数据信息的展示更为直观并且更深入挖掘数据潜在的价值,提出基于多框架集成的航空发动机高空模拟试验台数据流实时迁移及可视化技术。首先,利用MyBatis-Plus自动视图层构建可视化技术将存储于底层历史数据库中的信息直观地展示于Vue框架的浏览器端表结构空间中,方便试验员查看分析;然后利用基于GateWay限流机制的JDBC接口融合全量/增量和基于负载均衡机制的MyBatis框架融合多线程并行传输流的数据流迁移技术,将历史数据库中可供后续算法分析利用的重要数据信息同步至本地数据库中;最后,利用Vue框架的数据流前端监控界面,验证数据可视化展示的直观性、完整性、有效性和数据流同步的实时性。 展开更多
关键词 航空发动机高空模拟试验台 MyBatis自动视图层构建 GateWay限流机制 负载均衡机制
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A Duty Cycle-Based Gateway Selection Algorithm for LoRaWAN Downlink Communication
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作者 Mohammad Al Mojamed 《Computer Systems Science & Engineering》 SCIE EI 2023年第6期2953-2970,共18页
Long Range Wide Area Network(LoRaWAN)has been developed to meet the requirements for the enormous device-to-device communication of Inter-net of Things(IoT)networks,which consist of a large number of participating dev... Long Range Wide Area Network(LoRaWAN)has been developed to meet the requirements for the enormous device-to-device communication of Inter-net of Things(IoT)networks,which consist of a large number of participating devices spread over large coverage areas with low data rates and low power con-sumption.It supports communications in both directions,uplink,and downlink directions.However,the downlink communication in the current LoRaWAN raises the bottleneck issue at gateways due to the used gateway selection algo-rithm.This paper proposes a novel gateway selection algorithm based on the duty cycle time-off values for the existing gateways,Duty Cycle Gateway Selection(DCGS),to direct acknowledgment packets as downlink traffic towards the most suitable gateway.Thus,the proposed system avoids subsequent retransmission of previously sent traffic that leads to excessive traffic overloading the network.The proposed system avoids exhausting a gateway duty cycle with downlink traffic by distributing the downlink traffic among available gateways based on the duty cycle time off.DCGS is evaluated using FloRa and INET frameworks in the well-known network simulator OMNeT++.The result shows the superior perfor-mance of the proposed approach over the existing Signal-to-Noise ratio(SNR)based selection mechanism.It clearly indicates that the DCGS maintains a better confirmed packet delivery rate while reducing number of retransmissions,colli-sions,and power consumption. 展开更多
关键词 LoRa LoRaWAN gateway selection IOT DOWNLINK LPWAN
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Transparent Access to Heterogeneous IoT Based on Virtual Resources
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作者 Wenquan Jin Sunhwan Lim +2 位作者 Young-Ho Suh Chanwon Park Dohyeun Kim 《Computers, Materials & Continua》 SCIE EI 2023年第3期4983-4999,共17页
The Internet of Things(IoT)inspires industries to deploy a massive number of connected devices to provide smart and ubiquitous services to influence our daily life.Edge computing leverages sufficient computation and s... The Internet of Things(IoT)inspires industries to deploy a massive number of connected devices to provide smart and ubiquitous services to influence our daily life.Edge computing leverages sufficient computation and storage at the edge of the network to enable deploying complex functions closer to the environment using Internet-connected devices.According to the purpose of the environment including privacy level,domain functionality,network scale and service quality,various environment-specific services can be provided through heterogeneous applications with sensors and actuators based on edge computing.However,for providing user-friendly service scenarios based on the transparent access to heterogeneous devices in edge computing,a consistent interface shall be provided to deliver services from edge computing to clients.In this paper,we propose transparent computing based on virtual resources to access heterogeneous IoT devices without considering the underlying network configuration at the edge of the networks.For supporting transparent access to different edge computing environments through a consistent interface,the virtual resource of edge gateway is proposed to bridge the Internet and devices which are deployed on the edge of the network.The proposed edge gateway exposes the services of the Internet of Things devices to the Internet using virtual resources that represent the resources of physical devices.The virtual resources provide a consistent interface to enable clients to access devices in edge computing without considering underlying protocols.The virtual resource is generated by the resource directory in the edge gateway through the registration of a device.Based on the device registration,the device information is stored in the gateway to link virtual resources and devices for translating messages according to the destination protocols and identifying physical devices that are represented by virtual resources.Moreover,through collaboration with the service provider,the function of device discovery and monitoring is provided to clients. 展开更多
关键词 Internet of things edge computing edge gateway transparent computing VIRTUALIZATION resource directory virtual resource
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An Optimal Cluster Head and Gateway Node Selection with Fault Tolerance
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作者 P.Rahul B.Kaarthick 《Intelligent Automation & Soft Computing》 SCIE 2023年第2期1595-1609,共15页
In Mobile Ad Hoc Networks(MANET),Quality of Service(QoS)is an important factor that must be analysed for the showing the better performance.The Node Quality-based Clustering Algorithm using Fuzzy-Fruit Fly Optimiza-ti... In Mobile Ad Hoc Networks(MANET),Quality of Service(QoS)is an important factor that must be analysed for the showing the better performance.The Node Quality-based Clustering Algorithm using Fuzzy-Fruit Fly Optimiza-tion for Cluster Head and Gateway Selection(NQCAFFFOCHGS)has the best network performance because it uses the Improved Weighted Clustering Algo-rithm(IWCA)to cluster the network and the FFO algorithm,which uses fuzzy-based network metrics to select the best CH and entryway.However,the major drawback of the fuzzy system was to appropriately select the membership func-tions.Also,the network metrics related to the path or link connectivity were not considered to effectively choose the CH and gateway.When learning fuzzy sets,this algorithm employs a new Continuous Action-set Learning Automata(CALA)approach that correctly modifies and chooses the fuzzy membership functions.Despite the fact that it extends the network’s lifespan,it does not assist in the detection of defective nodes in the routing route.Because of this,a new Fault Tolerance(NQCAEFFFOCHGS-FT)mechanism based on the Distributed Connectivity Restoration(DCR)mechanism is proposed,which allows the net-work to self-heal as a consequence of the algorithm’s self-healing capacity.Because of the way this method is designed,node failures may be utilised to rebuild the network topology via the use of cascaded node moves.Founded on the fractional network information and topologic overhead related with each node,the DCR is suggested as an alternative to the DCR.When compared to the NQCAFFFOCHGS algorithm,the recreation results display that the proposed NQCAEFFFOCHGS-FT algorithm improves network performance in terms of end-to-end delay,energy consumption,Packet Loss Ratio(PLR),Normalized Routing Overhead(NRO),and Balanced Load Index(BLI). 展开更多
关键词 Hybrid-manet cluster head GATEWAY node failure fault tolerance distributed connectivity restoration
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AEECA for Reliable Communication to Enhance the Network Life Time for WSN
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作者 Ganesh Jayaraman V R Sarma Dhulipala 《Intelligent Automation & Soft Computing》 SCIE 2023年第2期1705-1719,共15页
Nowadays,wireless sensor networks play a vital role in our day to day life.Wireless communication is preferred for many sensing applications due its convenience,flexibility and effectiveness.The sensors to sense the en... Nowadays,wireless sensor networks play a vital role in our day to day life.Wireless communication is preferred for many sensing applications due its convenience,flexibility and effectiveness.The sensors to sense the environmental factor are versatile and send sensed data to central station wirelessly.The cluster based protocols are provided an optimal solution for enhancing the lifetime of the sensor networks.In this paper,modified K-means++algorithm is used to form the cluster and cluster head in an efficient way and the Advanced Energy-Efficient Cluster head selection Algorithm(AEECA)is used to calculate the weighted fac-tor of the transmission path and effective data collection using gateway node.The experimental results show the proposed algorithm outperforms the existing routing algorithms. 展开更多
关键词 Wireless sensor network reliable communication energy management energy clustering SENSORS base station gateway node
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Improved-Equalized Cluster Head Election Routing Protocol for Wireless Sensor Networks
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作者 Muhammad Shahzeb Ali Ali Alqahtani +5 位作者 Ansar Munir Shah Adel Rajab Mahmood Ul Hassan Asadullah Shaikh Khairan Rajab Basit Shahzad 《Computer Systems Science & Engineering》 SCIE EI 2023年第1期845-858,共14页
Throughout the use of the small battery-operated sensor nodes encou-rage us to develop an energy-efficient routing protocol for wireless sensor networks(WSNs).The development of an energy-efficient routing protocol is... Throughout the use of the small battery-operated sensor nodes encou-rage us to develop an energy-efficient routing protocol for wireless sensor networks(WSNs).The development of an energy-efficient routing protocol is a mainly adopted technique to enhance the lifetime of WSN.Many routing protocols are available,but the issue is still alive.Clustering is one of the most important techniques in the existing routing protocols.In the clustering-based model,the important thing is the selection of the cluster heads.In this paper,we have proposed a scheme that uses the bubble sort algorithm for cluster head selection by considering the remaining energy and the distance of the nodes in each cluster.Initially,the bubble sort algorithm chose the two nodes with the maximum remaining energy in the cluster and chose a cluster head with a small distance.The proposed scheme performs hierarchal routing and direct routing with some energy thresholds.The simulation will be performed in MATLAB to justify its performance and results and compared with the ECHERP model to justify its performance.Moreover,the simulations will be performed in two scenarios,gate-way-based and without gateway to achieve more energy-efficient results. 展开更多
关键词 Bubble sort algorithm GATEWAY energy thresholds wireless sensor networks
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欧盟“全球门户”战略在非洲的推进
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作者 潘多 王明进 《Contemporary International Relations》 2023年第2期63-78,共16页
At the 6th EU–AU Summit in February 2022,the European Exter nal Action Ser vice an nounced the EU–Africa: Global Gateway Investment Package, speeding up the implementation of the Global Gateway strategy in Africa. T... At the 6th EU–AU Summit in February 2022,the European Exter nal Action Ser vice an nounced the EU–Africa: Global Gateway Investment Package, speeding up the implementation of the Global Gateway strategy in Africa. The strategy seeks to counter the Belt and Road Initiative, consolidate Europe–Africa cooperation, repair Europe–Africa relations,and promote European values. However, advancing the Global Gateway strategy in Africa is constrained by geopolitical and value considerations, heavy historical burdens, unrealistic investment packages, and the uncertain sources of investment funds, thereby affecting African countries’ acceptance of the investment package.Therefore, advancing investment and cooperation in Africa based on the principle of noninterference in internal affairs and mutual benefit, rather than treating Africa as an arena for big power rivalries, will truly benefit, and be welcomed by, African countries. 展开更多
关键词 Global Gateway EU-Africa:Global Gateway Investment Package EU-Africa relations Belt and Road Initiative
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Widening the Gateway--China aims to foster internationally competitive manufacturing within an open environment
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作者 Mei Xinyu 《China Report ASEAN》 2023年第12期50-51,共2页
A mong the eight major steps China announced at the Third Belt and Road Forum for International Cooperation to support high-quality Belt and Road cooperation,the cancelation of all restrictions on foreign investment a... A mong the eight major steps China announced at the Third Belt and Road Forum for International Cooperation to support high-quality Belt and Road cooperation,the cancelation of all restrictions on foreign investment access in the manufacturing sector is the most striking measure,or at least one of the most eye-catching. 展开更多
关键词 INVESTMENT COMPETITIVE GATEWAY
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多种方式数据采集及通讯技术的需求分析
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作者 苗青 《工业控制计算机》 2023年第10期124-125,142,共3页
根据梳理工业现场仪器仪表及可编程控制器检测数据存在的各种数据通讯方式及市场需求,提出针对各种数据通讯的解决方案及设计要求,归纳汇总形成基本的多种数据采集通讯网关(Gateway),并统一格式进行终端数据库的上传。对目前市场上各种... 根据梳理工业现场仪器仪表及可编程控制器检测数据存在的各种数据通讯方式及市场需求,提出针对各种数据通讯的解决方案及设计要求,归纳汇总形成基本的多种数据采集通讯网关(Gateway),并统一格式进行终端数据库的上传。对目前市场上各种工业现场总线的数据采集及通讯提出了建设性建议。 展开更多
关键词 现场总线 工业数据通讯 通讯网关(Gateway)
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城市公路交通门户地区景观设计——以榆林市西门户景观设计为例(英文) 被引量:1
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作者 来朝旭 《Journal of Landscape Research》 2012年第6期1-3,6,共4页
Improving urban gateway landscape environment helps establish positive images of a city, and helps newcomers form proper recognition of the city. Landscape design of the west gateway in Yulin City was taken for exampl... Improving urban gateway landscape environment helps establish positive images of a city, and helps newcomers form proper recognition of the city. Landscape design of the west gateway in Yulin City was taken for example, its natural ecological environment, historical and humanistic environment and modern man-made environment were investigated, and also macro elements analyzed, such as overall urban planning, historical and cultural background of the city, regional environment features. It was to build the west gateway of Yulin City into a landscape transition area for experiencing culture and history of the city, and appreciating characteristic images of this region, and to improve natural ecological conditions of the local area, realize the sustainable development of regional environment. 展开更多
关键词 URBAN HIGHWAY TRAFFIC gateways LANDSCAPE design Space environment
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Gateway技术构建酵母双杂分析用辣椒cDNA文库 被引量:10
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作者 邱爱连 刘林林 +5 位作者 蔡汉阳 朱万里 黄木坤 陈雄 何水林 张健 《江西农业大学学报》 CAS CSCD 北大核心 2010年第4期791-796,共6页
构建cDNA文库是利用酵母双杂交技术开展特定cDNA分离的重要基础工作。利用Gateway技术,在成功提取辣椒总RNA并分离mRNA的基础上,合成了3种读码框的双链cDNA,通过BP反应构建其相应的入门文库,再通过LR反应将cDNA迅速且定向地重组到酵母... 构建cDNA文库是利用酵母双杂交技术开展特定cDNA分离的重要基础工作。利用Gateway技术,在成功提取辣椒总RNA并分离mRNA的基础上,合成了3种读码框的双链cDNA,通过BP反应构建其相应的入门文库,再通过LR反应将cDNA迅速且定向地重组到酵母双杂分析用目的载体pDESTTM22上,构建成高质量目的文库。经检测入门文库和目的文库滴度均达到(4.0~5.0)×106cfu/mL,文库总容量为(2.4~3.0)×107cfu,平均插入片段大小为1250bp,其质量完全满足于进一步利用酵母双杂体系分析目的基因互作蛋白cDNA。 展开更多
关键词 辣椒 入门文库 目的文库 GATEWAY 酵母双杂
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利用Gateway克隆技术大规模克隆拟南芥转录因子 被引量:19
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作者 梅文倩 宋文强 +2 位作者 潘怡 巩威 朱玉贤 《分子植物育种》 CAS CSCD 2004年第3期358-364,共7页
随着越来越多基因组全序列的测定完成,基因组的研究进入了功能研究阶段。功能基因组的研究需要把大量基因连入不同载体,传统的酶切连接构建载体的方法不能满足这种大规模克隆的需要。Gateway技术是一种高效的大规模克隆系统,而且对载体... 随着越来越多基因组全序列的测定完成,基因组的研究进入了功能研究阶段。功能基因组的研究需要把大量基因连入不同载体,传统的酶切连接构建载体的方法不能满足这种大规模克隆的需要。Gateway技术是一种高效的大规模克隆系统,而且对载体和宿主没有依赖性。本文利用Gateway大规模克隆技术将16个拟南芥转录因子的ORF克隆入植物表达载pPTV和酵母表达载体pYTV中,酵母融合表达实验和West-ern-blot检测证明了该克隆途径的可行性,并且得到的His-Tag融合蛋白,为拟南芥转录因子的大规模蛋白质组学研究奠定了良好的基础,同时基因序列的聚类分析为将来进一步的功能研究提供了有利信息。 展开更多
关键词 Gateway克隆技术 拟南芥 转录因子 聚类分析 功能基因组
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使用与Gateway技术兼容的T载体获得入门克隆 被引量:10
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作者 陈其军 安瑞 +2 位作者 周海梦 陈珈 王学臣 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2004年第10期951-954,共4页
与Gateway技术兼容的农杆菌双元载体系统已开始应用于植物功能基因组的研究 ,但应用这些载体系统的一个瓶颈问题 ,是如何简单、经济和高效地将PCR产物或其他来源的目的DNA片段构建到入门载体上获得入门克隆 .为此 ,将传统的TA克隆技术与... 与Gateway技术兼容的农杆菌双元载体系统已开始应用于植物功能基因组的研究 ,但应用这些载体系统的一个瓶颈问题 ,是如何简单、经济和高效地将PCR产物或其他来源的目的DNA片段构建到入门载体上获得入门克隆 .为此 ,将传统的TA克隆技术与Gateway重组克隆技术进行整合 ,构建了与Gateway技术兼容的两种TA克隆载体 ,用于在克隆PCR产物或其他来源的目的DNA片段的同时获得入门克隆 .利用兼容Gateway技术的TA克隆载体有效地解决了上述瓶颈问题 . 展开更多
关键词 Gateway克隆技术 TA克隆技术 入门载体 T载体 入门克隆
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