Acording to Avian Infectious Brochitis virus( IBV) Beaudette strain S1 gene sequence, a pair of primers were designed and synthesized. With the primers , IBV Guangdong isolation strain GD05 S1 gene was successively am...Acording to Avian Infectious Brochitis virus( IBV) Beaudette strain S1 gene sequence, a pair of primers were designed and synthesized. With the primers , IBV Guangdong isolation strain GD05 S1 gene was successively amplified by RT-PCR.PCR product was digested with BstY I,\%Hae\% III and \%Pst\% I respectively, the result showed RFLP pattern of was the same as that of M41 S1 gene. IBV GD05 strain was thought as Mass serotype primaryly. IBV GD05 S1 gene was cloned into pGEM-T vector and sequenced, its sequence was consisted of 1611 base pairs. By comparison , the nucleotide sequence was 97.14% identical to that of IBV M41. IBV GD05 S1 gene was subcloned into expression vector pET21d. SDS-PAGE experiment showed that it expressed in \%E.coli.\%展开更多
猫疱疹病毒1型（Feline Herpesvirus type 1，FHV—1）属于疱疹病毒科中的α-疱疹病毒，是猫病毒性鼻气管炎的病原，可引起猫及其他猫科动物的眼病及呼吸道疾病。猫疱疹病毒1型基因组编码多种蛋白质，其中7种糖蛋白gB、gC、gD、gC、gH...猫疱疹病毒1型（Feline Herpesvirus type 1，FHV—1）属于疱疹病毒科中的α-疱疹病毒，是猫病毒性鼻气管炎的病原，可引起猫及其他猫科动物的眼病及呼吸道疾病。猫疱疹病毒1型基因组编码多种蛋白质，其中7种糖蛋白gB、gC、gD、gC、gH、gI和gE已被鉴定。在这些糖蛋白中，gD糖蛋白具有高度的保守性和抗原性，能诱导机体产生细胞免疫和体液免疫，并能与细胞表面分子特异性结合，在病毒穿人易感细胞中起重要作用。展开更多
文摘Acording to Avian Infectious Brochitis virus( IBV) Beaudette strain S1 gene sequence, a pair of primers were designed and synthesized. With the primers , IBV Guangdong isolation strain GD05 S1 gene was successively amplified by RT-PCR.PCR product was digested with BstY I,\%Hae\% III and \%Pst\% I respectively, the result showed RFLP pattern of was the same as that of M41 S1 gene. IBV GD05 strain was thought as Mass serotype primaryly. IBV GD05 S1 gene was cloned into pGEM-T vector and sequenced, its sequence was consisted of 1611 base pairs. By comparison , the nucleotide sequence was 97.14% identical to that of IBV M41. IBV GD05 S1 gene was subcloned into expression vector pET21d. SDS-PAGE experiment showed that it expressed in \%E.coli.\%
文摘猫疱疹病毒1型（Feline Herpesvirus type 1，FHV—1）属于疱疹病毒科中的α-疱疹病毒，是猫病毒性鼻气管炎的病原，可引起猫及其他猫科动物的眼病及呼吸道疾病。猫疱疹病毒1型基因组编码多种蛋白质，其中7种糖蛋白gB、gC、gD、gC、gH、gI和gE已被鉴定。在这些糖蛋白中，gD糖蛋白具有高度的保守性和抗原性，能诱导机体产生细胞免疫和体液免疫，并能与细胞表面分子特异性结合，在病毒穿人易感细胞中起重要作用。
