Liangxue Xiaoban decoction and its disassembled prescriptions ameliorate psoriasis-like skin lesions induced by imiquimod in mice via T cell regulation

Objective:To explore the therapeutic capacity of the Liangxue Xiaoban(LXXB)decoction and its disassembled prescriptions in the modulation of T cell subsets and recurrence-related indexes of psoriasis using a psoriasis-like mouse model.Methods:The psoriasis model was generated by the treatment of BALB/c mice(n=48)with imiquimod.Mice were divided into six groups:control,psoriasis model,tripterygium glycosides,LXXB decoction,Liangxue decoction,and Qingqi decoction.After the intervention period,the interleukin(IL)-17A,IL-22,and interferon-γ levels in mice were examined and hematoxylin and eosin staining was conducted to determine pathological changes in the skin tissues.T cell subset changes in the skin-draining lymph nodes were analyzed using flow cytometry,and the expression levels of the associated transcription factors and recurrence-related indexes in the skin tissues were determined using a polymerase chain reaction.Results:LXXB decoction attenuated the levels of CD8^(+)T,Th17,and Th1 cells and induced an increase in the Th2 and Treg cell levels.The disassembled prescriptions promoted or inhibited specific subsets of T cells to improve the symptoms of psoriasis.Notably,the LXXB and Liangxue decoctions suppressed the expression of IL-22 at both the gene and protein levels and restored the CD103 and IL-15 expressions in the skin tissue to the normal range.Conclusion:LXXB decoction exerted significant immunoregulatory effects on T cell subsets and improved the recurrence-related indexes.Interestingly,the Liangxue prescription appeared to have a therapeutic advantage in terms of Th17 modulation and psoriasis recurrence,while the Qingqi prescription performed better in Treg immunoregulation.

Studies on a Traditional Chinese Medical Prescription Ma Xing Shi Gan Tang by Bio-coordination Chemistry

本文报道 pH 电位滴定法测出麻黄碱与生石膏中的 Cu^(2+)、Fe^(2+)、Zn^(2+)、Mn^(2+)离子形成的 ML 及 ML_2型络合物的稳定常数。滴定曲线显示甘草酸与上述四种金属离子也能分别形成不同稳定程度的络合物。用麻黄碱加甘草酸和上述四种金属离子配制成的模拟麻杏石甘汤和单纯生石膏煎剂与麻性石甘汤一样均有使家兔退热的效果,与对照组比较均有显著性差异(P<0.05);而用麻黄碱加甘草酸配制成的溶液无退热效果。作者认为应从生物—配位无机化学的多金属—多配体体系的观点去研究某些传统中医药方剂。

Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction,aspirin and itraconazole by UHPLC-FT-ICR-MS

A metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry(UHPLC-FT-ICR-MS)was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction(GQLD),aspirin and itraconazole.The differences in the small molecule profiles of treatment using traditional Chinese medicine,etiological treatment and symptomatic treatment were elucidated.Thirty-six plasma metabolites were identified or putatively identified,and the effects of the three medicines on the thirty-six metabolites were studied.Their metabolic pathways indicated that GQLD,aspirin and itraconazole ameliorated the rats with yeast-induced pyrexia predominantly by regulating the metabolisms of phospholipid,sphingolipid,fatty acid oxidation,fatty acid amides,amino acid and glycerolipid in vivo.The pharmacodynamics and metabonomic results showed that the three medicines exhibited the therapeutic effects on pyrexia by regulating the perturbations of multiple metabolisms.The study provided a scientific basis for an in-depth understanding of the therapeutic effects of GQLD,aspirin and itraconazole on rats with yeast-induced pyrexia.

TREATMENT OF INTRAPERITONEAL INFLAMMATORY MASSES WITH THE HEAT AND STAGNATION DISPELLING DECOCTION -A Report of 32 Cases

During 1985-1992,we used the Heatand Stagnation Dispelling Decoction (泻热逐瘀汤)in the treatment of 32 cases ofintraperitoneal inflammatory masses withgood results as reported in the following.

Research progress of Taohong Siwu Decoction and its modified prescription in the treatment of diabetic peripheral neuropathy

Western medicine mainly focuses on the conventional basic treatment of Diabetic peripheral neuropathy(DPN),such as blood glucose control,anti-oxidative stress,and nerve nutrition,but the efficacy and safety of the drugs cannot be guaranteed.As an important prescription for promoting blood circulation,removing blood stasis and nourishing blood,Taohong Siwu Decoction can not only promote cell proliferation and repair,reduce inflammatory factors in the blood,reduce the whole blood viscosity and plasma viscosity,and has a good anticoagulation effect.Moreover,Taohong Siwu Decoction and its added or subtracted prescription,foot bath and combined acupuncture have the effect of regulating blood sugar,improving nerve conduction velocity and relieving clinical symptoms,without obvious adverse reactions.This article reviews Taohong Siwu Decoction and its addition and subtraction in the treatment of diabetic peripheral neuropathy.

Mechanism of Yinchenhao decoction in the treatment of jaundice based on network pharmacology

Objective:This study aimed to examine the mechanism of classic ancient prescription of Chinese medicine Yinchenhao decoction in treating jaundice based on network pharmacology.Method:An oral bioavailability of≥30%,a drug likeness of≥0.18,and literature studies were used to screen for Yinchen(Artemisiae scopariae herba),Zhizi(Gardeniae fructus),Dahuang(Rhei radix et rhizome)in the Chinese Medicine System Pharmacology Database and Analysis Platform.The active ingredient was introduced into the PubChem database to collect drug component targets and import into the Uniprot database for gene standardization.The target gene of Yinchen(Artemisiae scopariae herba)was screened via Human Gene Database(GeneCards).Then,use the Cytoscape 3.7.2 software was used for network visualization analysis,and the R3.6.1 software was used for gene ontology functional and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses.Results:We collected a total of 47 active constituents of classic ancient prescription of Chinese medicine Yinchenhao decoction,of which 17 were related to jaundice;189,9 targets of jaundice were screened,of which 41 were interdigitated with the targets of classic ancient prescription of Chinese medicine Yinchenhao decoction.Gene ontology functional enrichment analysis revealed 111 biological processes,14 cellular components,and 28 molecular functions,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed 34 Kyoto Encyclopedia of Genes and Genomes pathways including hepatocellular carcinoma,PI3K-Akt signaling pathway,HIF-1 signaling pathway,prolactin signaling pathway,and non-alcoholic fatty liver disease.Conclusion:Based on the network pharmacology,the analysis of jaundice and classic ancient prescription of Chinese medicine Yinchenhao decoction provides a novel idea and direction for the study of classic ancient prescription of Chinese medicine Yinchenhao decoction in the treatment of jaundice.

Comparing the mechanism of four classic Gualou-Xiebai prescriptions for cardiovascular diseases with phlegm and blood stasis syndrome based on molecular network modeling

Background:Four classical Traditional Chinese Medicine prescriptions,namely Gualou Xiebai Baijiu decoction,Gualou Xiebai Banxia decoction(GLXBBX),Zhishi Xiebai Guizhi decoction(ZSXBGZ)and Danlou prescription(DL),have been frequently used for treatment of phlegm and blood stasis syndrome(PBSS)-related cardiovascular diseases.However,its therapeutic mechanism has not been clearly elucidated.This study aimed to explore PBSS and its molecular mechanism,clarify and compare the mechanisms of four prescriptions in treating PBSS-related diseases.Method:In this study,we collected four prescriptions’compounds,predicted therapeutic targets,and enriched pathways which were based on network pharmacology.Then,we analysed the commen and different mechanisms by combing the network of components,targets and pathways.Finally,molecular docking was engaged to assess the binding potential of key compounds and hub targets.Results:We showed that four prescriptions’intersection genes(VEGFA,SRC,EGFR,etc.)were commonly enriched in PI3K-AKT signaling pathway,HIF-1 signaling pathway,etc.In addition,platelet activation and cAMP signaling pathway were singly enriched from the GLXBBX through unique compounds 12,13-epoxy-9-hydroxynonadeca-7,10-dienoic acid and Cyclo(L-tyrosyl-L-phenylalanyl).These bioactive compounds may exert GLXBBX’s unique pharmacological pathways via involving in mediating PPARA,PTGER3,etc.Sphingolipid signaling pathway was singly enriched from the ZSXBGZ through unique compounds tetramethoxyluteolin,ergosterol peroxide,etc.These bioactive compounds could mediate ADORA1,ADORA3 and TNFRSF1A to regulate ZSXBGZ’s unique pharmacological pathways.AMPK signaling pathway was singly enriched from the DL through unique compounds kaempferol,evofolinb,ethyl acid and aureusidin.These bioactive compounds were involved in mediating the main targets of AMPK signaling pathway,such as TNF,TNFRSF1A,etc.Conclusions:Our research demonstrated that GLXB-prescriptions involved in almost all pathological stages of PBSS-related cardiovascular diseases by modulating high-frequency shared pathways and targets mainly through key compounds(quercetin,mandenol,sitosteryl acetate and luteolin,etc.),for example,participate in the process of atherosclerosis,lipid metabolism,inflammation,immune response,thrombosis,inhibit inflammatory factors and platelet aggregation,regulate immune function,vascular function,oxidative stress.In addition to common pharmacological efficacies,there could also be specificities among GLXB prescriptions due to different compounds.For example,GLXBBX tends to regulate the function of vascular and endothelial barrier,prevent thrombosis.ZSXBGZ tends to regulate lipid metabolism and protect the heart from lipid accumulation.DL tends to maintain energy homeostasis and improve inflammation.

Taohong Siwu Decoction:a classical Chinese prescription for treatment of orthopedic diseases

The pathogenesis of orthopedic diseases is intimately linked to blood stasis,frequently arising from damage to primary and secondary blood channels.This disruption can lead to“blood leaving the meridians”or Qi stagnation,resulting in blood stasis syndrome.Taohong Siwu Decoction(THSWD)is a renowned classical Chinese medicinal formula extensively used to promote blood circulation and mitigate blood stasis.Clinical studies have demonstrated its significant therapeutic effects on various orthopedic conditions,particularly its anti-inflammatory and analgesic properties,as well as its efficacy in preventing deep vein thrombosis post-surgery.Despite these findings,research on THSWD remains fragmented,and its interdisciplinary impact is limited.This review aims to provide a comprehensive evaluation of the efficacy and pharmacological mechanisms of THSWD in treating common orthopedic diseases.Additionally,we employ bibliometric analysis to explore research trends and hotspots related to THSWD.We hope this review will enhance the recognition and application of THSWD in orthopedic treatments and guide future research into its pharmacological mechanisms.

基于UHPLC-Q-Exactive Orbitrap MS技术分析经典名方芍药甘草汤的化学成分

目的基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用(UHPLC-Q-Exactive Orbitrap MS)技术对经典名方芍药甘草汤的化学成分进行定性分析。方法采用Thermo Scientific Hypersil GOLD C18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相梯度洗脱,流速为0.3 mL·min^(-1),柱温30℃。HESI-Ⅱ源正、负离子2种模式采集芍药甘草汤的一级、二级质谱数据。结果根据高分辨质谱提供的精确分子量和碎片离子信息,结合标准品比对、软件预测分析和相关文献报道对芍药甘草汤的化学成分进行分析。正、负离子模式下共鉴定出129个化合物,包括82个黄酮类化合物,20个萜类化合物,10个酚类化合物,7个香豆素类化合物和10个其他类化学成分。结论利用UHPLC-Q-Exactive Orbitrap MS技术分析鉴定芍药甘草汤中的化学成分,并初步归纳其各类主要化学成分的质谱裂解特点,可为芍药甘草汤的质量控制以及药效物质基础的深入研究提供实验依据。

经典名方麦门冬汤化学成分的UPLC-Q-Orbitrap-MS分析

目的 采用超高效液相色谱-四极杆-静电轨道阱-高分辨质谱(UPLC-Q-Exactive Orbitrap MS)对麦门冬汤的化学成分进行快速分析和鉴定。方法 采用Waters CORTECS T3 C_(18)色谱柱(2.1 mm×150 mm,1.6μm),以0.1%甲酸水(A)-乙腈(B)为流动相进行梯度洗脱,体积流量为0.3 mL/min。质谱采用ESI源,正负离子模式分别采集一级、二级质谱数据。结果 通过对照品指认、软件预测分析、结合文献报道、质谱裂解规律分析,从麦门冬汤中共鉴定出141个化学成分,包括黄酮及其苷类54个、高异黄酮类27个、三萜类40个、甾体皂苷类7个、苯丙素类7个和其他类6个。结论 采用UPLC-Q-Orbitrap-MS技术可以系统、快速地鉴定麦门冬汤全方的物质基础,为该复方进一步药效物质基础研究及质量控制的建立提供了实验依据。

OBSERVATIONS ON THE EFFICACY OF AN ACARICIDAL LOTION OF TRADITIONAL CHINESE MEDICINE IN THE TREATMENT OF ROSACEIFORM DERMATITIS

In search of an effective treatment forrosaceiform dermatitis,we made a clinicaltrial on patients with the condition by usingan acaricidal prescription of traditionalChinese medicine worked out by us,and ob-tained satisfactory results.The work issummarized as follows.Clinical MaterialA total of 50 cases were treated,in-cluding 17 males and 33 females.The pa-tients ranged in age from 16 to 62 years

The clinical application of Yanghe Decoction

Yanghe Decoction is a representative prescription of such prescriptions as warming up the yang and dissipating the cold.It has the functions of warming the blood and dispelling cold and stagnation.Yanghe Decoction has effects on many diseases.The principle of this kind of disease is blood deficiency and yang,and the clinical manifestation is cold stagnation.Such diseases often occur in multiple systems and in multiple aspects.This article summarizes the compatibility of Yanghe Decoction and its clinical application by reviewing the related literature analysis in recent years,and provides a broad idea for the application of Yanghe Decoction.

Clinical Effect of Fuyang Prescription in Treatment of Ischemic Stroke during the Convalescent Stage:An Analysis of 25 Cases

[Objectives]The paper was to investigate the clinical effect of Fuyang prescription in the treatment of yang deficiency syndrome in the convalescent stage of ischemic stroke(IS)and its mechanism of action.[Methods]A total of 50 patients with yang deficiency syndrome in the convalescent stage of IS were randomly divided into treatment group and control group,with 25 patients in each group.The patients in the treatment group were given basic treatment combined with Fuyang prescription,and those in the control group were given basic treatment combined with Buyang Huanwu decoction;the course of treatment was 28 d for both groups.The activities of daily living(ADL)score,National Institutes of Health Stroke Scale(NIHSS)score,quality of life score,TCM syndrome score,and safety in both groups were observed before treatment and at 14 and 28 d post treatment,and their clinical outcome was evaluated.[Results]There was a significant difference in overall response rate between the treatment group and the control group[92.00%(23/25)vs 76.00%(19/25),P<0.05].After treatment,both groups had significant changes in ADL score,NIHSS score,quality of life score,and TCM syndrome score,and there were significant differences in these indices between the two groups at the same time point after treatment(P<0.05 or P<0.01).There were no significant changes in routine blood,routine stool,routine urine,and liver/renal function in either group after treatment.[Conclusions]Fuyang prescription has a marked clinical effect in the treatment of yang deficiency syndrome in the convalescent stage of IS,with significantly better clinical effect than Buyang Huanwu decoction,and it provides clinical evidence for the traditional Chinese medicine theory of yang supporting in the treatment of IS in the convalescent stage.

New Clinical Application of Chaihu Decoction

The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been systematically studied.The author applied Chaihu Wendan decoction to treat phlegm and qi stagnation in the middle energizer,applied Chaihu Guizhi decoction to treat simultaneous disease of Shaoyang and Taiyang,applied Chaihu Xiexin decoction to treat simultaneous disease of the spleen,stomach,liver,and gallbladder,applied Chaihu Ermiao powder to treat simultaneous disease of hand Shaoyang and foot Taiyin,applied Chaihu Erchen decoction to treat simultaneous disease of Shaoyang and Taiyin,applied Chailing decoction to treat simultaneous disease of hand Shaoyin and foot Taiyang,applied Chaihu Guizhi Ganjiang decoction to treat Shaoyang and Taiyin cold fluid retention and heat stagnation syndrome of Shaoyang complicated with Taiyin,applied Chaihu Xingren decoction to treat simultaneous disease of hand Shaoyang and hand Taiyin,applied Chaihu Sanren decoction to treat the disease caused by damp-heat stagnation in the hand Shaoyang and the foot Taiyin,and applied Chaihu Ganlu Xiaodu pellet to treat the diseases of hand Shaoyang and foot Yangming.The effects were all remarkable.

葛根芩连汤通过IRS-1/PI3K/AKT通路对胃肠湿热型2型糖尿病大鼠糖脂代谢的影响

目的探究葛根芩连汤通过胰岛素受体底物-1(IRS-1)/磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)通路对胃肠湿热型2型糖尿病大鼠糖脂代谢的影响。方法将40只SD大鼠随机分为正常组(2 mL生理盐水灌胃)、造模组(2 mL生理盐水灌胃)、二甲双胍组(4.17 mg/100 g二甲双胍灌胃)和葛根芩连汤组(1 g/100 g葛根芩连汤灌胃),每组10只。采用高脂高糖饲料加腹腔注射链脲佐菌素(STZ)构建2型糖尿病大鼠模型,随后喂食油脂、42°白酒及蜂蜜水构建胃肠湿热型2型糖尿病大鼠模型。测量各组大鼠不同时间节点体质量,血糖仪测定空腹血糖(FBG);ELISA检测空腹胰岛素(FINS)、三酰甘油(TG)、总胆固醇(TC)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平变化、计算胰岛素抵抗指数(HOMA-IR);HE染色检测肝组织病理学变化;检测肝组织过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量变化。Western blot检测肝组织IRS-1、PI3K、p-PI3K、AKT及p-AKT蛋白变化。结果与正常组比较,造模组大鼠体质量、FBG、FINS及HOMA-IR、GSH-Px、CAT、SOD、IRS-1、p-PI3K/PI3K及p-AKT/AKT水平均明显下降(P<0.05)、TG、TC、IL-6、TNF-α及MDA含量均显著升高(P<0.05),可见局灶性肝实质损失。与造模组比较,二甲双胍组及葛根芩连汤组大鼠体质量、FBG、FINS及HOMA-IR、GSH-Px、CAT、SOD、IRS-1、p-PI3K/PI3K及p-AKT/AKT水平均明显升高(P<0.05)、TG、TC、IL-6、TNF-α及MDA含量均显著降低(P<0.05),显示正常的肝实质。结论葛根芩连汤可明显改善胃肠湿热型2型糖尿病糖脂紊乱,可能是通过IRS-1/PI3K/AKT通路发挥作用。

Explanation of Gegen Qinlian Decoction Syndrome and Its Clinical Application

Gegen Qinlian Decoction is one of the commonly used classical prescriptions,which consists of fourherbs:Gegen(Puerariae Lobatae Radix),Huangqin(Scutellariae Radix),Huanglian(Coptidis Rhizoma),and Zhigancao Glycyrrhizae Radix et Rhizoma Praeparata cum Melle.The clinical application experience of Gegen Qinlian Decoction is as follows:first,according to Zhongjing Zhang's original text,heat distressing the large intestine with unresolved exterior syndrome is theoriginal meaningof Gegen Qinlian Decoction syndrome.In clinical practice,bacillary dysentery,gastrointestinal cold,etc.,belong to simultaneous exterior and interior disease,which are completely consistent with the original meaning of this prescription;second,the clinical digestive system diseases with exuberant heat of the large intestine as the core manifestation cannot be treated merely based on the exterior syndrome;third,due to the interior-exterior relationship between the lung and the large intestine and between the meridians,the use of Gegen(Puerariae Lobatae Radix),Huangqin(Scutellariae Radix),and Huanglian(Coptidis Rhizoma)can make the source of body clear,and the waste qi can be released and thepore can be easily opened,so this prescription can also be used for exogenous fever;fourth,with multidimensional comprehensive understanding of the syndrome,pathogenesis,symptoms and pharmacology,Gegen Qinlian Decoction has also been further applied to the treatment of hypertension,diabetes,etc.

半夏泄心汤全方及其拆方联合美沙拉嗪治疗溃疡性结肠炎的临床疗效及对炎性因子水平的影响

目的:探究半夏泄心汤全方及其拆方联合美沙拉嗪治疗溃疡性结肠炎的应用效果。方法:选取2023年5月~2024年5月在吉林省人民医院消化科和中医科诊治的120例中度溃疡性结肠炎患者作为研究对象。随机分为对照组(24例)与观察组(96例)。观察组分为全方组、辛开组、苦降组、甘补组,每组24例。对照组与观察组同时给药美沙拉嗪肠溶片。连续治疗3个月,比较对照组与观察组治疗前后的肠道内窥镜及外周血血清炎性因子水平。记录药物的不良反应。结果:观察组与对照组均表现出不同程度的治疗效果。对照组美沙拉嗪可以通过抑制炎性反应达到治疗结肠炎的作用。半夏泻心汤及拆方联合美沙拉嗪治疗结肠炎表现出良好的效果。辛开组具有温暖中焦脾胃的功效,能够偏向缓解患者呕吐,腹痛等症状。苦降组由于具有较多生物碱、黄酮类成分,具有较强的止泻、抗菌、消炎的功效。甘补组虽然能够大补元气提高患者免疫力,但由于患者肠道功能退化,并不能起到良好的效果。结论:半夏泻心汤全方联合美沙拉嗪可以有效缓解结肠炎症状并提高疗效。不同组方偏向不同,但单纯运用甘补类药材,不适宜肠胃功能较差的患者。

经典名方真武汤的历史沿革及临床应用规律

目的 考证真武汤的历史沿革和应用规律,为其深入研究提供精准的文献参考。方法 以真武汤为关键词,从《中华医典》检索、整理该方1991年1月以前的古籍。以真武汤、Zhenwu decoction、Zhenwu tang为主题词从中国知网、万方数据服务知识平台、维普网和Pub Med中获取2002年1月至2022年12月的临床研究文献。考证真武汤的处方来源、药物组成、剂量、炮制、功能主治、病证要素和应用规律。结果 纳入古籍92部,真武汤首载于《伤寒论》,由茯苓、芍药、白术、附子、生姜组成。剂量以茯苓三两、芍药三两、白术二两、附子一枚、生姜三两居多。在炮制方面,芍药以生白芍为主,白术以炒用为主,附子以熟附子为主。在应用规律方面,真武汤主要治疗少阴病和太阳病,涉及的症状有腹痛、小便不利、四肢疼痛、大便自利等,脾肾阳虚、阳虚水泛、心肾阳虚是临床应用真武汤的辨证要点。真武汤的应用涉及心血管、泌尿、消化等多系统,包括心力衰竭、糖尿病肾病、肾病综合征等疾病。结论本研究体现了真武汤的传承性、稳定性、有效性和安全性,为其研发提供了科学、客观的文献依据。

蒙药乌兰—13味汤方源考证及研究概况

蒙药乌兰—13味汤(WL—13)最早记载于《通瓦嘎吉德》,之后收录于1998年版《中华人民共和国卫生部药品标准》(蒙药分册)中。WL—13由栀子、诃子、川楝子、土木香、山柰、悬钩木、苦参、茜草、紫草、紫草茸、枇杷叶、金莲花、橡子等13种单味药材配伍组成,具有清血热的功效,主治血热盛行、头痛、目赤、高血压、成熟热与未成熟热、感冒、白脉病、肺热、鼻出血、皮疹、中暑、风湿、类风湿等诸疾,具有良好的潜在研究价值。本综述探析了WL—13的历史沿革、方解、临床应用及实验研究进展,以期为该药的现代化研究提供理论支撑。

羌活胜湿汤物质基准多指标成分含量测定及量值传递分析

[目的]建立经典名方羌活胜湿汤(QSD)物质基准的高效液相(HPLC)含量测定方法,进行量值传递分析。[方法]制备15批QSD物质基准冻干粉,以羌活醇、二氢欧山芹醇当归酸酯、5-O-甲基维斯阿米醇苷、蔓荆子黄素、异欧前胡素、甘草酸铵为专属性指标成分,建立含量测定的检测方法,明确指标成分的含量范围、转移率范围及出膏率,对饮片到物质基准对应实物间的量值传递进行分析。[结果]15批物质基准指标成分含量(转移率)分别为:羌活醇1.09~1.70 mg/g(10.17%~32.62%),二氢欧山芹醇当归酸酯0.06~0.20 mg/g(7.30%~10.85%),5-O-甲基维斯阿米醇苷1.27~2.81 mg/g(66.13%~92.13%),蔓荆子黄素0.09~0.59 mg/g(5.24%~13.37%),异欧前胡素0.03~0.28 mg/g(0.94%~1.92%),甘草酸铵12.74~33.78 mg/g(48.96%~62.56%);出膏率为30.09%~34.35%。[结论]采用HPLC法初步建立了经典名方QSD物质基准多指标成分含量测定方法,为经典名方QSD后续的研究及相关制剂研发的质量控制提供依据。