For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their paren...For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorerline R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis(2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimentalmaterials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterileline (Wu-jin2A, WujinSA) by comparing to restorer line (R168, R988) and the first filial (F, )generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pl5.8-6.5, molecular weight 42. 7 X 10~3-66. 2 X 10~3.展开更多
This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant serve...This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant served as"bait"in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S.mutans with Fusobacterium nucleatum(F.nucleatum),Streptococcus mitis(S.mitis),Streptococcus gordonii(S.gordonii),Streptococcus sanguis(S.sanguis),Actinomyces naeslundii(A.naeslundii)and Lactobacillus.Co-adherence assays were also performed using unfractionated saliva from healthy individuals.Co-adhering partners of S.mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Both UA159 and its srt A-deficient mutant bound to F.nucleatum but not to any of the other five salivary bacteria.The srt A-deficient mutant showed lower co-adherence with F.nucleatum.The two S.mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria,except that UA159 S.mutans but not the srt A-deficient bound to a Neisseria sp.under the same conditions.Deleting srt A reduces the ability of S.mutans to bind to F.nucleatum,but it does not appear to significantly affect the binding profile of S.mutans to bulk salivary bacteria.展开更多
文摘For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorerline R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis(2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimentalmaterials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterileline (Wu-jin2A, WujinSA) by comparing to restorer line (R168, R988) and the first filial (F, )generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pl5.8-6.5, molecular weight 42. 7 X 10~3-66. 2 X 10~3.
基金supported by grants from the National Natural Science Foundation of China(No.81570974)the Key Project of the Science and Technology Department of Sichuan Province(No.2015JY0260)
文摘This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant served as"bait"in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S.mutans with Fusobacterium nucleatum(F.nucleatum),Streptococcus mitis(S.mitis),Streptococcus gordonii(S.gordonii),Streptococcus sanguis(S.sanguis),Actinomyces naeslundii(A.naeslundii)and Lactobacillus.Co-adherence assays were also performed using unfractionated saliva from healthy individuals.Co-adhering partners of S.mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Both UA159 and its srt A-deficient mutant bound to F.nucleatum but not to any of the other five salivary bacteria.The srt A-deficient mutant showed lower co-adherence with F.nucleatum.The two S.mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria,except that UA159 S.mutans but not the srt A-deficient bound to a Neisseria sp.under the same conditions.Deleting srt A reduces the ability of S.mutans to bind to F.nucleatum,but it does not appear to significantly affect the binding profile of S.mutans to bulk salivary bacteria.
