Objective:To summarize the precise association between pulmonary tuberculosis(PTB) and P2x7 A1513 C gene polymorphism.Methods:PubMed and Google Scholar web-databases were searched for the studies reporting the associa...Objective:To summarize the precise association between pulmonary tuberculosis(PTB) and P2x7 A1513 C gene polymorphism.Methods:PubMed and Google Scholar web-databases were searched for the studies reporting the association of P2x7 A1513 C polymorphism and PTB risk.A meta-analysis was performed for the selected case-control studies and pooled odds ratios(ORs) and 95%confidence intervals(95%CIs) were calculated for all the genetic models.Results:Eleven studies comprising 2 678 controls and 2 113 PTB cases were included in this meta-analysis.We observed overall no significant risk in all the five genetic models.When stratified population by the ethnicity,Caucasian population failed to show any risk of PTB in all the genetics models.In Asian ethnicity,variant allele(C vs.A:P=0.001;QR=1.375,95%CI=1.159-1.632) and heterozygous genotype(AC vs.AA:P=0.001;OR=1.570,95%CI=1.269-1.944) demonstrated significant increased risk of PTB.Likewise,recessive genetic model(CC+AC vs.AA:P=0.001;OR=1.540,95%CI= 1.255-1.890) also demonstrated increased risk of PTB in Asians.Conclusions:Our meta-analysis did not suggest the association of P2x7 A1513 C polymorphism with PTB risk in overall or separately in Caucasian population.However,it plays a significant risk factor for predisposing PTB in Asians.Future larger sample and expression studies are needed to validate this association.展开更多
Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used...Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used for many applications. In this study, HRM assay was developed and evaluated for the detection of PZA resistance in Mycobacterium tuberculosis clinical isolates. Methods: Ninety five M. tuberculosis clinical isolates with different susceptibility patterns to anti-TB drugs were used to evaluate this assay. Isolates were phenotypically (Bactec MGIT 960) and genotypically (HRM and pncA gene sequencing) analysed for PZA resistance. Results: Bactec MGIT 960 analysis revealed that 29 of the 95 M. tuberculosis isolates were PZA resistant. In comparison to the Bactec MGIT 960, HRM showed a sensitivity of 47.7% and specificity of 74.6%, and the overall agreement between the two methods was 68.4%. Based on DNA sequencing, a correlation of 0.67 (significant at p-value pncA mutations was observed. PZA resistance was strongly associated with multi-drug resistant (MDR)-TB as it was shown in 79.3% of the MDR isolates included in the study. Conclusion: HRM is simple and useful for screening clinical M. tuberculosis isolates for PZA resistance, however, further modifications to improve its performance are required.展开更多
目的 分析维生素D受体基因多态性与肺结核易感性的病例对照研究的结果。方法应用Meta—analysis，以Fleiss模型和DerSimonian and Nan Laird模型（D—L模型）对6项研究结果进行数据处理。结果累计病例841例，对照1419例，采用Fleiss模...目的 分析维生素D受体基因多态性与肺结核易感性的病例对照研究的结果。方法应用Meta—analysis，以Fleiss模型和DerSimonian and Nan Laird模型（D—L模型）对6项研究结果进行数据处理。结果累计病例841例，对照1419例，采用Fleiss模型计算的合并OR值ff vs FF为1．20（0．84-1．71），Ff vs FF为0．99（0．81～1.21），D—L模型计算的合并OR值ff vs FF为1．12（0．67-1．86），Ff vs FF为0．99（0．80-1.23）。失效安全数小于1。结论尚不能认为维生素D受体基因多态性是肺结核易感性的影响因素。展开更多
目的揭示结核分枝杆菌（Mycobacterium tuberculosis，MTB）北京基因型菌株的进化路径，在进化过程中产生的各个进化分支，以及每个分支的北京基因型菌株在人群中的流行情况。方法收集2014年1月至2016年4月天津地区临床分离的567株MTB...目的揭示结核分枝杆菌（Mycobacterium tuberculosis，MTB）北京基因型菌株的进化路径，在进化过程中产生的各个进化分支，以及每个分支的北京基因型菌株在人群中的流行情况。方法收集2014年1月至2016年4月天津地区临床分离的567株MTB菌株，首先采用多重PCR试验分析菌株基因组中差异片段207（RD207）的缺失情况，以鉴定收集的菌株是否为北京基因型；然后分析所有的北京基因型MTB菌株基因组中差异区域RD105、RD181、RD150和RD142的缺失情况，以及菌株基因组NTF（noise transfer function）区中插入序列6110（IS6110）的存在情况。结果567株临床分离的MTB菌株中，517株（91．2％）为北京基因型菌株。所有北京基因型菌株中，447株（86．5％）为NTF区含有IS6110的北京基因型现代株；70株（13．5％）为NTF区不含IS6110的北京基因型古代株。基于大片段的多态性分析，北京基因型菌株被分为5个亚型，其中RD181（＋）的北京基因型菌株为22株（4．3％），且全部为北京基因型古代株。RD181（-）／RD150（＋）和RD181（-）／RD150（-）的北京基因型古代株分别为41株（7．9％）和7株（1．4％）。447株现代菌株中，RD181（-）／RD150（＋）和RD181（-）／RD150（-）的分别为404株（78．1％）和43株（8．3％）。结论MTB北京基因型中的现代株是天津地区的主要流行株；北京基因型MTB在人群传播流行中已经进化出5个分支，其中RD181（-）／RD150（＋）的北京基因型现代株为主要的流行分支。展开更多
目的筛选并验证抗结核药物所致肝损伤(anti-tuberculosis drug-induced hepatotoxicity,ATDH)与精神分裂症(schizophrenia,SCZ)之间可能的共同致病基因。方法通过查询基因数据库及文献,分别确定ATDH和SCZ的致病基因,利用重启动随机游走(...目的筛选并验证抗结核药物所致肝损伤(anti-tuberculosis drug-induced hepatotoxicity,ATDH)与精神分裂症(schizophrenia,SCZ)之间可能的共同致病基因。方法通过查询基因数据库及文献,分别确定ATDH和SCZ的致病基因,利用重启动随机游走(integrating network random walk with restart,RWR)、基因富集分析(gene set enrichment analysis,GSEA)和超几何检验等生物信息学分析技术筛选出两种疾病的可能共同致病基因,建立定期更新的两种疾病共同致病基因在线数据库(www.bio-x.cn/atdh-sczgenes.html),查阅文献以进一步筛选与ATDH和SCZ都有关的基因;利用meta分析等方法验证候选基因,以最终确定共同致病基因。结果经信息学分析技术筛选,共获得500个候选共同致病基因。进一步经文献检索,得到GSTM1和GSTT1基因为候选共同致病基因并纳入meta分析。Meta分析显示,GSTM1基因同ATDH(P=0.01)和SCZ(P<0.01)相关均有统计学意义;而GSTT1基因同ATDH和SCZ的发病风险关系均无统计学意义(P>0.05)。结论 GSTM1基因为ATDH和SCZ的共同致病基因。展开更多
文摘Objective:To summarize the precise association between pulmonary tuberculosis(PTB) and P2x7 A1513 C gene polymorphism.Methods:PubMed and Google Scholar web-databases were searched for the studies reporting the association of P2x7 A1513 C polymorphism and PTB risk.A meta-analysis was performed for the selected case-control studies and pooled odds ratios(ORs) and 95%confidence intervals(95%CIs) were calculated for all the genetic models.Results:Eleven studies comprising 2 678 controls and 2 113 PTB cases were included in this meta-analysis.We observed overall no significant risk in all the five genetic models.When stratified population by the ethnicity,Caucasian population failed to show any risk of PTB in all the genetics models.In Asian ethnicity,variant allele(C vs.A:P=0.001;QR=1.375,95%CI=1.159-1.632) and heterozygous genotype(AC vs.AA:P=0.001;OR=1.570,95%CI=1.269-1.944) demonstrated significant increased risk of PTB.Likewise,recessive genetic model(CC+AC vs.AA:P=0.001;OR=1.540,95%CI= 1.255-1.890) also demonstrated increased risk of PTB in Asians.Conclusions:Our meta-analysis did not suggest the association of P2x7 A1513 C polymorphism with PTB risk in overall or separately in Caucasian population.However,it plays a significant risk factor for predisposing PTB in Asians.Future larger sample and expression studies are needed to validate this association.
文摘Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used for many applications. In this study, HRM assay was developed and evaluated for the detection of PZA resistance in Mycobacterium tuberculosis clinical isolates. Methods: Ninety five M. tuberculosis clinical isolates with different susceptibility patterns to anti-TB drugs were used to evaluate this assay. Isolates were phenotypically (Bactec MGIT 960) and genotypically (HRM and pncA gene sequencing) analysed for PZA resistance. Results: Bactec MGIT 960 analysis revealed that 29 of the 95 M. tuberculosis isolates were PZA resistant. In comparison to the Bactec MGIT 960, HRM showed a sensitivity of 47.7% and specificity of 74.6%, and the overall agreement between the two methods was 68.4%. Based on DNA sequencing, a correlation of 0.67 (significant at p-value pncA mutations was observed. PZA resistance was strongly associated with multi-drug resistant (MDR)-TB as it was shown in 79.3% of the MDR isolates included in the study. Conclusion: HRM is simple and useful for screening clinical M. tuberculosis isolates for PZA resistance, however, further modifications to improve its performance are required.
文摘目的 分析维生素D受体基因多态性与肺结核易感性的病例对照研究的结果。方法应用Meta—analysis，以Fleiss模型和DerSimonian and Nan Laird模型（D—L模型）对6项研究结果进行数据处理。结果累计病例841例，对照1419例，采用Fleiss模型计算的合并OR值ff vs FF为1．20（0．84-1．71），Ff vs FF为0．99（0．81～1.21），D—L模型计算的合并OR值ff vs FF为1．12（0．67-1．86），Ff vs FF为0．99（0．80-1.23）。失效安全数小于1。结论尚不能认为维生素D受体基因多态性是肺结核易感性的影响因素。
文摘目的揭示结核分枝杆菌（Mycobacterium tuberculosis，MTB）北京基因型菌株的进化路径，在进化过程中产生的各个进化分支，以及每个分支的北京基因型菌株在人群中的流行情况。方法收集2014年1月至2016年4月天津地区临床分离的567株MTB菌株，首先采用多重PCR试验分析菌株基因组中差异片段207（RD207）的缺失情况，以鉴定收集的菌株是否为北京基因型；然后分析所有的北京基因型MTB菌株基因组中差异区域RD105、RD181、RD150和RD142的缺失情况，以及菌株基因组NTF（noise transfer function）区中插入序列6110（IS6110）的存在情况。结果567株临床分离的MTB菌株中，517株（91．2％）为北京基因型菌株。所有北京基因型菌株中，447株（86．5％）为NTF区含有IS6110的北京基因型现代株；70株（13．5％）为NTF区不含IS6110的北京基因型古代株。基于大片段的多态性分析，北京基因型菌株被分为5个亚型，其中RD181（＋）的北京基因型菌株为22株（4．3％），且全部为北京基因型古代株。RD181（-）／RD150（＋）和RD181（-）／RD150（-）的北京基因型古代株分别为41株（7．9％）和7株（1．4％）。447株现代菌株中，RD181（-）／RD150（＋）和RD181（-）／RD150（-）的分别为404株（78．1％）和43株（8．3％）。结论MTB北京基因型中的现代株是天津地区的主要流行株；北京基因型MTB在人群传播流行中已经进化出5个分支，其中RD181（-）／RD150（＋）的北京基因型现代株为主要的流行分支。
文摘目的筛选并验证抗结核药物所致肝损伤(anti-tuberculosis drug-induced hepatotoxicity,ATDH)与精神分裂症(schizophrenia,SCZ)之间可能的共同致病基因。方法通过查询基因数据库及文献,分别确定ATDH和SCZ的致病基因,利用重启动随机游走(integrating network random walk with restart,RWR)、基因富集分析(gene set enrichment analysis,GSEA)和超几何检验等生物信息学分析技术筛选出两种疾病的可能共同致病基因,建立定期更新的两种疾病共同致病基因在线数据库(www.bio-x.cn/atdh-sczgenes.html),查阅文献以进一步筛选与ATDH和SCZ都有关的基因;利用meta分析等方法验证候选基因,以最终确定共同致病基因。结果经信息学分析技术筛选,共获得500个候选共同致病基因。进一步经文献检索,得到GSTM1和GSTT1基因为候选共同致病基因并纳入meta分析。Meta分析显示,GSTM1基因同ATDH(P=0.01)和SCZ(P<0.01)相关均有统计学意义;而GSTT1基因同ATDH和SCZ的发病风险关系均无统计学意义(P>0.05)。结论 GSTM1基因为ATDH和SCZ的共同致病基因。
