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Protein transduction domain of membrane penetrating peptide can efficiently deliver DNA and protein into mouse liver for gene therapy 被引量:4
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作者 Jun Xie, Bao-Feng Yu, Jun Xu, Yue-Hong Zhang, Niu-Liang Cheng, Bo Niu, Xiao-Nian Hu, Qian Xiang and Zheng-Guo Zhang Taiyuan, China Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001 , China Peking Union Medical College, Chi- nese Academy of Medical Sciences, Beijing 100005, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2005年第1期90-93,共4页
BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of ... BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector's function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system. 展开更多
关键词 membrane penetrating peptide gene therapy gene delivering
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Polycation-functionalized gold nanodots with tunable near-infrared fluorescence for simultaneous gene delivery and cell imaging 被引量:1
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作者 Yuanqing Sun Dandan Wang +6 位作者 Yueqi Zhao Tianxin Zhao Hongchen Sun Xiangwei Li Chuanxi Wang Bai Yang Quan Lin 《Nano Research》 SCIE EI CAS CSCD 2018年第5期2392-2404,共13页
Near-infrared (NIR) fluorescent metal nanodots may have significant advantages in biological detection and bioimaging. Herein, we introduce tunable near-infrared fluorescent gold nanodots (AuNDs) protected by bran... Near-infrared (NIR) fluorescent metal nanodots may have significant advantages in biological detection and bioimaging. Herein, we introduce tunable near-infrared fluorescent gold nanodots (AuNDs) protected by branched polyethylenimine (PEI) modified by surface segmental attachment of sulfhydryl groups (PEI-SH), abbreviated as PEI-SH-AuNDs, for simultaneous gene delivery and cell imaging. The modified PEI endows the resultant PEI-SH-AuNDs with the following excellent advantages. Sulfhydryl groups of PEI-SH anchor to the surface of AuNDs, and such polycations with amine groups give PEI-SH-AuNDs remarkable stability. The cationic polymer PEI-SH with positive charges enables PEI-SH-AuNDs to perform gene delivery, and the gene transfection efficiency can reach 22.8%. Moreover, the fluorescence of PEI-SH-AuNDs is tunable from visible red light (wavelength 609 nm) to NIR light (wavelength 811 run) via an increase in the size of AuNDs. PEI-SH-AuNDs yielded gene transfection efficiency similar to that of commercial PEI, but showed much lower cytotoxicity and much greater red-shift fluorescence. With excellent photoluminescent properties, such multifunctional fluorescent PEI-SH-AuNDs hold promise in applications to bioimaging and as ideal fluorescent probes for tracking gene transfection behavior. 展开更多
关键词 near-infrared fluorescent material Au nanodot tunable fluorescence gene deliver BIOIMAGING
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