Objectives To detect the polymorphism of D5S436 locus, and to carry out an amplified fragment length polymorphism (Amp FLP) genotyping linkage analysis in asthma families.Methods Allele and genotype frequencies of ...Objectives To detect the polymorphism of D5S436 locus, and to carry out an amplified fragment length polymorphism (Amp FLP) genotyping linkage analysis in asthma families.Methods Allele and genotype frequencies of the high polymorphic D5S436 locus were determined in 92 unrelated Chinese individuals by using polymerase chain reaction (PCR). The amplified fragments were separated by denaturing polyacrylamide gel electrophoresis and silver staining techniques. Results Eight alleles and 22 genotypes were observed in this population. The heterozygosity was 78.26%. The polymorphism information content (PIC) was 0.76. Amp FLP genotyping linkage analyses were carried out in four asthma families, demonstrating that the locus was inherited according to Mendel's law and had a clear result.Conclusion The PIC of D5S436 is high in Chinese individuals, so it could be used as a genetic marker of asthma in Chinese and might be useful in the gene diagnosis of asthma.展开更多
为使偏分离群体连锁图矫正方法能被广泛应用,在Microsoft Visual Studio 2010操作平台下,利用C++语言研制了Windows界面友好的DistortedMap软件包。结果显示:DistortedMap软件包具有遗传群体模拟、偏分离群体连锁图矫正和数量性状基因...为使偏分离群体连锁图矫正方法能被广泛应用,在Microsoft Visual Studio 2010操作平台下,利用C++语言研制了Windows界面友好的DistortedMap软件包。结果显示:DistortedMap软件包具有遗传群体模拟、偏分离群体连锁图矫正和数量性状基因座定位的功能。使用该软件包可获得偏分离标记间遗传距离的无偏估计值并消除标记偏分离对QTL定位的影响。展开更多
文摘Objectives To detect the polymorphism of D5S436 locus, and to carry out an amplified fragment length polymorphism (Amp FLP) genotyping linkage analysis in asthma families.Methods Allele and genotype frequencies of the high polymorphic D5S436 locus were determined in 92 unrelated Chinese individuals by using polymerase chain reaction (PCR). The amplified fragments were separated by denaturing polyacrylamide gel electrophoresis and silver staining techniques. Results Eight alleles and 22 genotypes were observed in this population. The heterozygosity was 78.26%. The polymorphism information content (PIC) was 0.76. Amp FLP genotyping linkage analyses were carried out in four asthma families, demonstrating that the locus was inherited according to Mendel's law and had a clear result.Conclusion The PIC of D5S436 is high in Chinese individuals, so it could be used as a genetic marker of asthma in Chinese and might be useful in the gene diagnosis of asthma.
文摘为使偏分离群体连锁图矫正方法能被广泛应用,在Microsoft Visual Studio 2010操作平台下,利用C++语言研制了Windows界面友好的DistortedMap软件包。结果显示:DistortedMap软件包具有遗传群体模拟、偏分离群体连锁图矫正和数量性状基因座定位的功能。使用该软件包可获得偏分离标记间遗传距离的无偏估计值并消除标记偏分离对QTL定位的影响。