为了探究胎盘印记和营养转运基因与新生牛初生重的相关性,以鉴定新生牛生长发育候选标记基因,试验选取初生重为26.5~31.5 kg和32.0~36.0 kg的新生健康布莱凯特黑牛各3头,分别记为低初生重组(LW组)和高初生重组(HW组),采集两组的胎盘组...为了探究胎盘印记和营养转运基因与新生牛初生重的相关性,以鉴定新生牛生长发育候选标记基因,试验选取初生重为26.5~31.5 kg和32.0~36.0 kg的新生健康布莱凯特黑牛各3头,分别记为低初生重组(LW组)和高初生重组(HW组),采集两组的胎盘组织并提取RNA,利用实时荧光定量PCR分析两组胎盘中印记基因[水通道蛋白1(aquaporin 1,AQP1)、小核核糖核蛋白多肽N(small nuclear ribonucleoprotein polypeptide N,SNRPN)、致命-3恶性脑肿瘤样蛋白1(lethal-3 malignant brain tumor-like protein 1,L3MBTL1)、pleckstring同源性样结构域家族A成员2(pleckstring homology-like domain family A member 2,PHLDA2)、胰岛素样生长因子2(insulin like growth factor 2,IGF2)、生长因子受体结合蛋白10(growth factor receptor bound protein 10,GRB10)]和营养转运基因[溶质载体家族38成员10(solute carrier family 38 member 10,SLC38A10)、葡萄糖转运蛋白家族2成员1(solute carrier family 2 member 1,SLC2A1)、葡萄糖转运家族2蛋白2(solute carrier family 2 member 2,SLC2A2)、脂肪酸转运蛋白家族27成员1(solute carrier family 27 member 1,SLC27A1,也称FATP1)、质子依赖性胃肠肽转运蛋白1(proton-dependent gastrointestinal peptide transporter 1,PEPT1,也称SLC15A1)、脂肪酸转运蛋白家族27成员4(solute carrier family 27 member 4,SLC27A4,也称FATP4)、钠依赖型葡萄糖转运载体1(Na+/glucose cotransporter 1,SGLT1,也称SLC5A1)、白细胞分化抗原36(cluster of differentiation 36,CD36)]的mRNA表达水平差异,并分析上述基因表达量与初生重间的相关性,最后对上述基因进行生物信息学分析。结果表明:LW组平均初生重[(30.00±0.87)kg]和HW组[(34.17±1.17)kg]差异显著(P<0.05);胎盘印记基因AQP1、SNRPN、L3MBTL1、PHLDA2、IGF2、GRB10在HW组和LW组胎盘中均有表达,其中HW组AQP1、SNRPN、L3MBTL1、PHLDA2基因mRNA相对表达量显著高于LW组(P<0.05),IGF2、GRB10基因显著低于LW组(P<0.05)。胎盘营养转运基因SLC38A10、PEPT1、SGLT1、SLC2A1、SLC2A2、CD36、FATP1、FATP4在HW组和LW组胎盘中均有表达,其中HW组SLC2A2、FATP1基因mRNA相对表达量显著高于LW组(P<0.05);HW组SLC38A10、PEPT1、CD36、FATP4基因mRNA相对表达量则显著低于LW组(P<0.05);SLC2A2基因mRNA相对表达量在HW组和LW组胎盘中均最高,然后依次为CD36、FATP1基因,SLC2A1基因最低。AQP1、SNRPN、L3MBTL1、SLC2A2和FATP1基因表达量与初生重呈正相关,相关系数分别为0.671,0.858,0.779,0.704,0.492,其中印记基因SNRPN的相关系数最高,表明其与新生牛初生重相关性最高;GRB10、SLC38A10、FATP4、PEPT1、CD36基因表达量与初生重呈现较强负相关,相关系数分别为-0.857,-0.896,-0.961,-0.989,-0.819。AQP1蛋白位于蛋白互作网络中心,与SNRPN、SLC2A2、SLC2A1、IGF2等蛋白的相互作用较强。14个印记和营养转运基因分别注释到碳水化合物跨膜转运、葡萄糖跨膜转运、长链脂肪酸导入细胞等生物学过程条目,顶端质膜、基质膜等细胞组分条目,碳水化合物跨膜转运蛋白活性、葡萄糖跨膜转运蛋白活性等分子功能条目;注释到碳水化合物的消化和吸收、脂肪细胞因子信号通路等通路。说明AQP1、SNRPN、SLC2A2、CD36等胎盘印记和营养转运基因可能通过调控胎盘发育及葡萄糖转运影响新生牛发育。展开更多
Obesity and nonalcoholic steatohepatitis are increasing in westernized countries, regardless of their geographiclocation. In Latin America, most countries, including Mexico, have a heterogeneous admixture genome with ...Obesity and nonalcoholic steatohepatitis are increasing in westernized countries, regardless of their geographiclocation. In Latin America, most countries, including Mexico, have a heterogeneous admixture genome with Amerindian, European and African ancestries. However, certain high allelic frequencies of several nutrientrelated polymorphisms may have been achieved by past gene-nutrient interactions. Such interactions may have promoted the positive selection of variants adapted to regional food sources. At present, the unbalanced diet composition of the Mexicans has led the country to a 70% prevalence rate of overweightness and obesity due to substantial changes in food habits, among other factors. International guidelines and intervention strategies may not be adequate for all populations worldwide because they do not consider disparities in genetic and environmental factors, and thus there is a need for differential prevention and management strategies. Here, we provide the rationale for an intervention strategy for the prevention and management of obesity-related diseases such as nonalcoholic steatohepatitis based on a regionalized genome-based diet. The components required to design such a diet should focus on the specific ancestry of each population around the world and the convenience of consuming traditional ethnic food.展开更多
文摘为了探究胎盘印记和营养转运基因与新生牛初生重的相关性,以鉴定新生牛生长发育候选标记基因,试验选取初生重为26.5~31.5 kg和32.0~36.0 kg的新生健康布莱凯特黑牛各3头,分别记为低初生重组(LW组)和高初生重组(HW组),采集两组的胎盘组织并提取RNA,利用实时荧光定量PCR分析两组胎盘中印记基因[水通道蛋白1(aquaporin 1,AQP1)、小核核糖核蛋白多肽N(small nuclear ribonucleoprotein polypeptide N,SNRPN)、致命-3恶性脑肿瘤样蛋白1(lethal-3 malignant brain tumor-like protein 1,L3MBTL1)、pleckstring同源性样结构域家族A成员2(pleckstring homology-like domain family A member 2,PHLDA2)、胰岛素样生长因子2(insulin like growth factor 2,IGF2)、生长因子受体结合蛋白10(growth factor receptor bound protein 10,GRB10)]和营养转运基因[溶质载体家族38成员10(solute carrier family 38 member 10,SLC38A10)、葡萄糖转运蛋白家族2成员1(solute carrier family 2 member 1,SLC2A1)、葡萄糖转运家族2蛋白2(solute carrier family 2 member 2,SLC2A2)、脂肪酸转运蛋白家族27成员1(solute carrier family 27 member 1,SLC27A1,也称FATP1)、质子依赖性胃肠肽转运蛋白1(proton-dependent gastrointestinal peptide transporter 1,PEPT1,也称SLC15A1)、脂肪酸转运蛋白家族27成员4(solute carrier family 27 member 4,SLC27A4,也称FATP4)、钠依赖型葡萄糖转运载体1(Na+/glucose cotransporter 1,SGLT1,也称SLC5A1)、白细胞分化抗原36(cluster of differentiation 36,CD36)]的mRNA表达水平差异,并分析上述基因表达量与初生重间的相关性,最后对上述基因进行生物信息学分析。结果表明:LW组平均初生重[(30.00±0.87)kg]和HW组[(34.17±1.17)kg]差异显著(P<0.05);胎盘印记基因AQP1、SNRPN、L3MBTL1、PHLDA2、IGF2、GRB10在HW组和LW组胎盘中均有表达,其中HW组AQP1、SNRPN、L3MBTL1、PHLDA2基因mRNA相对表达量显著高于LW组(P<0.05),IGF2、GRB10基因显著低于LW组(P<0.05)。胎盘营养转运基因SLC38A10、PEPT1、SGLT1、SLC2A1、SLC2A2、CD36、FATP1、FATP4在HW组和LW组胎盘中均有表达,其中HW组SLC2A2、FATP1基因mRNA相对表达量显著高于LW组(P<0.05);HW组SLC38A10、PEPT1、CD36、FATP4基因mRNA相对表达量则显著低于LW组(P<0.05);SLC2A2基因mRNA相对表达量在HW组和LW组胎盘中均最高,然后依次为CD36、FATP1基因,SLC2A1基因最低。AQP1、SNRPN、L3MBTL1、SLC2A2和FATP1基因表达量与初生重呈正相关,相关系数分别为0.671,0.858,0.779,0.704,0.492,其中印记基因SNRPN的相关系数最高,表明其与新生牛初生重相关性最高;GRB10、SLC38A10、FATP4、PEPT1、CD36基因表达量与初生重呈现较强负相关,相关系数分别为-0.857,-0.896,-0.961,-0.989,-0.819。AQP1蛋白位于蛋白互作网络中心,与SNRPN、SLC2A2、SLC2A1、IGF2等蛋白的相互作用较强。14个印记和营养转运基因分别注释到碳水化合物跨膜转运、葡萄糖跨膜转运、长链脂肪酸导入细胞等生物学过程条目,顶端质膜、基质膜等细胞组分条目,碳水化合物跨膜转运蛋白活性、葡萄糖跨膜转运蛋白活性等分子功能条目;注释到碳水化合物的消化和吸收、脂肪细胞因子信号通路等通路。说明AQP1、SNRPN、SLC2A2、CD36等胎盘印记和营养转运基因可能通过调控胎盘发育及葡萄糖转运影响新生牛发育。
文摘Obesity and nonalcoholic steatohepatitis are increasing in westernized countries, regardless of their geographiclocation. In Latin America, most countries, including Mexico, have a heterogeneous admixture genome with Amerindian, European and African ancestries. However, certain high allelic frequencies of several nutrientrelated polymorphisms may have been achieved by past gene-nutrient interactions. Such interactions may have promoted the positive selection of variants adapted to regional food sources. At present, the unbalanced diet composition of the Mexicans has led the country to a 70% prevalence rate of overweightness and obesity due to substantial changes in food habits, among other factors. International guidelines and intervention strategies may not be adequate for all populations worldwide because they do not consider disparities in genetic and environmental factors, and thus there is a need for differential prevention and management strategies. Here, we provide the rationale for an intervention strategy for the prevention and management of obesity-related diseases such as nonalcoholic steatohepatitis based on a regionalized genome-based diet. The components required to design such a diet should focus on the specific ancestry of each population around the world and the convenience of consuming traditional ethnic food.
