Genes Expressing Differences in Upland Cotton Fiber Development Between 12 DPA and 16 DPA

Cotton is the major dominant natural fiber crop on the earth.Although some current cotton genetics and breeding programs had made great progresses in cotton lint yield increases and disease resistance(tolerance),fiber quality has little improvement.Global understanding genes expression

Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin

LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里，我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响， Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽，我们观察到 E-cadherin 的表示，与肿瘤转移联系的一个必要粘附分子，被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而，抑制被雌激素剥夺废除，显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对，建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。

Clinicopathological significance of LRP16 protein in 336 gastric carcinoma patients

AIM:To investigate the expression of leukemia related protein 16 (LRP16), and the possible relationship between LRP16 expression and clinicopathological indices in 336 gastric carcinoma patients.METHODS: Immunohistochemistry was used to detect LRP16 expression in 336 cases of paraffin-embedded gastric carcinoma tissues and 60 cases of distal normal mucosa. The relationships between LRP16 expression and patients' age, tumor size, histological grade, clinical stage, metastatic status and prognosis were analysed.RESULTS: The expression of LRP16 was 58.6% (197/336) in gastric carcinoma and 31.7% (19/60) in distal normal gastric mucosa. The expression of LRP16 in carcinoma was significantly higher than that in normal mucosa tissues (χ2=14.929, P=0.001). LRP16 protein expression was found in 44.1% (63/143) carcinomas at stage and,and 69.4%(134/193) carcinomas at stage and (χ2=21.804,P=0.001),and in 56.9%(182/320)of cancers without metastasis but 93.8%(15/16)of those with metastasis(χ2=8.543,P=0.003).The expression of LRP16 was correlated with tumor size,infiltrative depth,clinical stage,lymphatic invasion and distant metastasis(all P<0.05). Follow-up data showed that there was a significant difference in median survival time between cancer patients with expression of LRP16 (27.0 mo) and those without (48.0 mo, Log rank=31.644, P=0.001).CONCLUSION: The expression of LRP16 may be associated with invasion, metastasis and prognosis of gastric cancer.

Reduced Expression of the LRP16 Gene in Mouse Insulinoma (MIN6) Cells Exerts Multiple Effects on Insulin Content, Proliferation and Apoptosis

This study assessed the effects of leukemia-related protein 16 (LRP16) on the regulation of pancreatic functions in mouse insulinoma (MIN6) cells. Cells with down-regulated expression of LRP16 were obtained by a shRNA interference strategy. Insulin content and glucose-stimulated insulin secretion (GSIS) were examined by radioimmunoassay. Western blotting was applied to detect protein expression. Glucose-stimulated sub-cellular localization of PDX-1 was immunocytochemically determined. Cell proliferation and apoptosis were detected by flow cytometry. Our results showed that LRP16 regulated insulin content in MIN6 cells by controlling expression of insulin and insulin transcription factors. LRP16 gene silence in MIN6 cells led to reduced cell proliferation and increased apoptosis. The observation of phosphorylation of serine-473 Akt and the localization of PDX-1 to the nucleus under glucose-stimulation exhibited that LRP16 was a component mediating Akt signaling in MIN6 cells. These results suggest that LRP16 plays a key role in maintaining pancreatic β-cell functions and may help us to understand the protective effects of estrogen on the functions of pancreatic β-cells.

Molecular Characterization and Virulence Genes of Aeromonas hydrophila Isolated from the Chinese Giant Salamander (Andrias davidianus)

The Chinese giant salamander(Andrias davidianus) is the largest living amphibian in the world. Aeromonas hydrophila strain L602 was isolated from A. davidianus. The 16S rDNA gene of this isolate was amplified using PCR,and the phylogenetic tree was constructed by the neighbor-joining method. Four virulence genes(aerA,aha1,hly and alt) of A. hydrophila were amplified by PCR and drug resistances were tested using Kirby-Bauer disk diffusion method. The results showed that the length of this 16S rDNA sequence was 1453 bp,which showed 99% homology with A. hydrophila. The GenBank accession number was JX155398. Phylogenetic analysis indicated it grouped together with A. hydrophila. Four virulence genes were all detected,indicating that strain L602 was highly virulent. This stain was resistant to four antibiotics(vibramycin,furazolidone,ampicillin and erythromycin),while it was insensitive to streptomycin. Furthermore,this strain was susceptible to six antibiotics(sulfafurazole,ciprofloxacin,penbritin,norfloxacin,florfenicol and enrofloxacin). This study will help to validate the classification and virulence of pathogenic bacteria in amphibians.

Estrogen Receptor α(ERα) Target Gene LRP16 Interacts with ERα and Enhances Receptor's Transcriptional Activity

Objective：It has been shown that LRP16 is an estrogen-induced gene through its receptor α（ERα）. Although there is evidence demonstrating that inhibition of LRP16 gene expression in MCF-7 human breast cancer cells partially attenuates its estrogen-responsiveness, the underlying molecular mechanism is still unclear. Here, the effect of LRP16 expression on the ERα signaling transduction was investigated. Methods： Cotransfection assays were used to measure the effect of LRP16 on ERα-mediated transcriptional activity. GST-pulldown and immunoprecipitation （ColP） assays were employed to investigate the physical interaction of LRP16 and ERα. The mammalian two-hybrid method was used to map the functional interaction region. Results： the results of cotransfection assays demonstrated that the transcriptional activities of ERα were enhanced in α LRP16 dose-dependent manner in MCF-7 in the presence of estrogen, however, it was abolished in the absence of E2 in MCF-7 cells. The physical interaction of LRP16 and ERα proteins was confirmed by GST-pulldown in vitro and ColP in vivo assays, which was enhanced by E2 but not dependent on its presence. Furthermore, the results of the mammalian two-hybrid assays indicated that the binding region of ERα to LRP16 located at the A/B AF-1 functional domain and E2 stimulated the binding of LRP16 to the full-length ERα molecule but not to the A/B region alone. Conclusion： These results support a role for estrogenically regulated LRP16 as an ERα coactivator, providing a positive feedback regulatory loop for ERα signal transduction. Based on this function of LRP16, we propose that ERα-positive breast cancer patients with high expression of LRP16 might benefit from targeting LRP16 therapy.

16S rRNA Gene Phylogenesis of Culturable Predominant Bacteria from Diseased Apostichopus japonicus(Holothuroidea,Echinodermata)

Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease,pathogens of which are supposed to be bacteria by most researchers,is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao,China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies,and touchdown PCR was performed to amplify the target sequences. The results suggest that γ-proteobacteria(Alteromonadales and Vibrionales) of CFB group,many strains of which have been also determined as pathogens in other marine species,are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.

INHIBITION OF PROLIFERATION OF HUMAN BREAST CANCER MCF-7 CELLS BY SMALL INTERFERENCE RNA AGAINST LRP16 GENE

Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferation. Here, the effects of the LRP16 gene expression on growth of MCF-7 human breast cancer cells and the mechanism were further studied by establishing two stably LRP16-inhibitory MCR-7 cell lines. Methods: Hairpin small interference RNA (siRNA) strategy, by which hairpin siRNA was released by U6 promoter and was mediated by pLPC-based retroviral vector, was adopted to knockdown endogenous LRP16 level in MCF-7 cells. And the hairpin siRNA against green fluorescence protein (GFP) was used as the negative control. The suppressant efficiency of the LRP16 gene expression was confirmed by Nothern blot. Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation. Cell cycle checkpoints including cyclin E and cyclin D1 were examined by Western blot. Results: The results from cell proliferation assays suggested that down-regulation of LRP16 gene expression is capable of inhibiting MCF-7 breast cancer cell growth and down-regulation of the LRP16 gene expression is able to inhibit anchorage-independent growth of breast cancer cells in soft agar. We also demonstrated that cyclin E and cyclin D1 proteins were much lower in the LRP16-inhibitory cells than in the control cells. Conclusion: These data suggest that LRP16 gene play an important role in MCF-7 cells proliferation by regulating the pathway of the G1/S transition and may function as an important modulator in regulating the process of tumorigenesis in human breast.

ESTROGEN REGULATION OF LRP16 GENE EXPRESSION INVOLVES SP1 TRANSCRIPTION FACTOR

Objective： To investigate the role of Spl as transcription factor required for transactivation of LRP16 gene by estrogen. Methods： Specific antibodies of ERα and Spl were used to precipitate the target DNA/protein complexes of MCF-7 cells at different time points after estrogen treatment （Chromatin immunoprecipitation assay）, the promoter region of LRP16 gene was amplified by semi-nested polymerase chain reaction （snPCR）. Small interfering RNA （siRNA） against Spl was transiently cotransfected with LRP16-Luc （containing the region from -213bp to -126bp of LRP16 gene promoter）in MCF-7 cells. The luciferase activities were measured by dual-luciferase assay. Results： The results of chromatin immunoprecipitation assay showed that Spl protein directly bound to the -213bp to -126bp region of LRP16 gene, and ERα could enhance the affinity of Spl to DNA. Spl-siRNA specifically decreased the transactivation of LRP16-Luc by 1713-estradio1 to 70-80%. Conclusion： The estrogen-induced transactivation of the human LRP16 gene was mediated by Spl protein. Moreover, the interactions of ERα/Sp1 functional complex with LRP16 promoter DNA were required for enhanced LRP16 gene transactivation.

Biodiversity of <i>Bacillus thuringiensis</i>Strains and Their <i>Cry</i>Genes in Ecosystems of Kyrgyzstan

The present study aims to isolate the unknown and known serotypes of Bacilllus thuringiensis (Bt) from natural objects in Kyrgyzstan. A total of 83 Bt strains were isolated from natural substrates, of which 30% were taken from the soil and litter samples, 69.7% from dead insects and about 0.3% from slugs. Serological examination revealed that such subspecies as var. thuringiensis (H-1), var. alesti (H-3), var. sotto (H-4a4b) and var. entomocidus (H-6) predominated in the upper horizon of soils in all climatic zones. In the dead insects such species as subsp. thuringiensis, subsp. galleria, subsp. sotto, subsp. kurstaki, subsp. Aizawai and subsp. Entomocidus dominated. A set of Bt strains isolated from insects and soil samples, selected from different ecosystems in Kyrgyzstan was molecular taxonomically characterized using the pycA gene as marker for phylogenetic reconstruction. Within the Bacillus cereus sensu lato species complex, all Kyrgyz isolates were shown to belong to the B. cereus subspecies thuringiensis. Most isolates were assigned to the lineage Bt tolworthi, with two isolates each belonging to the lineages Bt kurstaki and Bt sotto. A high degree of cry gene diversity was demonstrated in the set of Bt isolates, with several gene copies simultaneously present in a single strain;a particularly conspicuous trait was the frequent combination of Lepidopteran-specific cryI with Dipteran-specific cryIV genes in the same Bt isolate.

The prevalence and distribution of aminoglycoside resistance genes

Choosing the appropriate antibiotics to treat bacterial infections has grown more challenging as a result of the emergence of antibiotic-resistant bacteria.Aminoglycosides,as broad-spectrum antibiotics,are increasingly being used clinically;however,for most effective employment of aminoglycosides,a comprehensive understanding of aminoglycoside resistance genes’prevalence and dissemination is required.Therefore,to better understand the global resistance status of aminoglycoside antibiotics and the prevalence of antibiotic-resistance genes(ARGs)in various bacterial species,this systematic review gathered relevant data from multiple studies.Two primary resistance mechanisms-aminoglycoside enzymatic modification and 16S rRNA methylation-were assessed,and the prevalence of the corresponding ARGs was described.The coexistence of aminoglycoside ARGs with other ARGs was also demonstrated,as was the relationship between aminoglycoside ARGs and resistant phenotypes.The lack of effective therapeutic agents to combat resistant pathogens presents a real threat to public health.The combination of aminoglycosides with other antibiotics may provide a novel treatment strategy.

基于线粒体16S rRNA基因的鹅肉源性成分鉴别方法研究

研究旨在建立基于线粒体16S rRNA基因的鹅源性成分鉴别方法。试验以鹅源性DNA为阳性模板,以猪、牛、羊、鸽、鹌鹑、火鸡、鸡和鸭等8个物种DNA为干扰模板的混合模板,设计筛选出鹅特异性引物,进行PCR和荧光定量PCR(qPCR)反应,并将鹅肉DNA模板浓度按101~1088个梯度进行稀释,检测方法灵敏度。结果显示:所设计的引物仅对鹅肉DNA有特异性扩增,对鹅以外的其他8个物种均没有扩增;当鹅肉DNA模板稀释104倍,PCR扩增条带仍然清晰;当稀释倍数达到107时,仍有较好的扩增曲线,且Ct值小于35。研究表明,建立的畜禽肉中鹅源性成分PCR和qPCR鉴别方法不仅具有良好的特异性,而且具有较高的灵敏性,为食品中鹅源性成分的鉴别提供了新途径。

LRP16基因在乳腺癌组织中的表达及其临床意义

背景与目的:既往研究表明雌激素通过其受体!直接上调LRP16的表达,LRP16基因的高表达促进乳腺癌细胞的增殖。本研究旨在探讨LRP16基因在乳腺癌组织中的表达状况及其与临床病理特征的关系。方法:收集52例乳腺癌组织及其配对癌旁组织,Northernblot与半定量RT-PCR法分别检测22例和30例标本中LRP16mRNA水平。免疫组化法检测肿瘤组织中雌激素受体(ER)、孕激素受体(PR)及Ki-67的表达情况。结果:Northernblot检测结果表明,22例癌组织LRP16mRNA的表达较癌旁组织高2倍的有9例,高表达率为40.9%(9/22)。高表达LRP16的9例中有7例ER阳性,8例PR阳性;而非高表达13例中ER阳性6例,PR阳性5例,两组ER与PR阳性率均有显著性差异(P<0.05)。LRP16高表达的9例中只有1例PR和ER同时阴性,而非高表达的13例中有7例。22例患者中,13例肿瘤直径3.0～4.5cm,其中LRP16基因高表达组占8例;有腋窝淋巴结转移的12例中8例LRP16高表达。8例高表达Ki-67患者中6例LRP16高表达。半定量RT-PCR检测结果表明,30例肿瘤标本中9例(30.0%)LRP16mRNA的表达水平明显高于癌旁组织,9例LRP16高表达者ER、PR均阳性,Ki-67高表达,且瘤体直径均大于3.5cm,均有腋窝淋巴结转移,与非高表达组比较差异有显著性(P<0.05)。结论:LRP16基因表达水平与ER/PR阳性率、细胞增殖活性、肿瘤直径、远处淋巴结转移密切相关,提示LRP16基因可能参与促进乳腺癌的增殖与转移。

一个新的白血病相关基因LRP16全长cDNA的克隆、序列分析及表达特征

克隆一个与白血病复发相关基因 (LRP1 6)的全长 c DNA序列 ,对其进行染色体定位、组织表达谱分析 ,并对该基因编码蛋白质进行原核表达 .首先用获得的一段 3kb DNA片段在 NCBI提供的 h ESTs数据库中进行电子杂交并对重叠克隆片段组装 ,再设计引物进行 c DNA末端快速扩增(RACE技术 ) .采用 Northern印迹方法进行组织表达分析 .以高通量基因组序列 (HTGS)数据库为基础进行染色体定位 .对构建的克隆菌用 IPTG诱导重组蛋白表达后进行 SDS- PAGE,同时对重组体测序确证 .钓取了该基因全长 c DNA、推导所编码的氨基酸序列 ,并将该基因定位于染色体1 1 q1 2 .2 .原核表达筛选获得了该基因重组子的一个缺失体 .对 LRP1 6基因全长 c DNA的序列分析提示 ,该基因可能编码两种 N端不同的蛋白质 ,且该基因的转录本可能存在一种丰度较低的剪接体 .

LRP16对乳腺癌MCF-7细胞增殖的影响

用Northern印迹方法检测雌二醇 (17β E2 )对LRP16mRNA表达的时间及剂量依赖性调控作用 .构建LRP16基因启动子序列调控的萤光素酶报告子 (pS0 ) ,并与雌激素受体α和 β(ERα和ERβ)表达载体共转染COS 7和MCF 7细胞后测定萤光素酶活性 .将LRP16基因的表达载体转染MCF 7细胞 ,测定过表达LRP16对细胞的生长特性的影响 .17β E2 使MCF 7细胞中LRP16mRNA表达水平增加 ,增加幅度未显示出 17β E2 培养时间和剂量的依赖性 .pS0 与ERα表达载体共转染细胞的相对萤光素酶活性较非共转染组 (对照组 )及pS0 ERβ表载体共转染组升高 5～ 10倍 .LRP16基因过表达促进MCF 7细胞的增殖 .研究表明 ,雌激素可能通过ERα上调乳腺癌MCF

RACE技术在钓取白血病相关基因LRP16全长cDNA中的应用

LRP16是我们应用甲基化敏感的限制性界标基因组扫描 (restrictionlandmarkgenomicscanning ,RLGS)技术时发现的一个与白血病复发相关的新基因。为钓取这一新基因的全长cDNA ,本实验采用了cDNA末端快速扩增法 (rapidamplificationofcDNAend ,RACE)。通过对RACE法若干步骤进行优化 ,克隆得到了LRP16基因cDNA的 5′ 与 3′ 非翻译区 ,进而获得其全长及完整的开放阅读框架 ,并作为新基因被GenBank收录。上述结果表明 ,RACE技术是钓取未知基因全长cDNA敏感而快速的方法 ,尤其是对 5′ 及 3′

ERα与Sp1相互作用激活LRP16启动子转录活性

根据已报道的LRP1 6启动子序列 (2 6kb) ,采用PCR反应获得 6个启动子 5′删除突变体 ,分别插入pGL3 Basic载体 ,构建 6种 5′缺失报告基因表达载体 (pS1 ～pS6) .分别与ERα真核表达载体共转染MCF 7细胞 .用双荧光素酶报告系统测定荧光素酶活性 ,以明确LRP1 6基因上游启动子区域中的雌激素反应序列 .结果显示 ,pS1 ～pS6均有雌二醇反应性 .进而对pS5的 3′端缺失分析发现LRP1 6基因翻译起始位点上游 - 2 1 4至 - 2 5 1位置的序列具有雌激素应答 ,序列分析发现该片段序列中包含了一个供转录因子Sp1结合的GC富含位点和一个ERα反应元件的半位点 (1 2ERE Sp1 ) ,进一步突变分析显示这两个元件均为雌激素反应性所必需 .以含这两个顺式元件的序列 (- 2 5 3bp至 - 2 2 4bp)作为探针 ,超级迁移凝胶电泳试验结果表明了ERα和Sp1蛋白均可以和探针结合 .研究发现了雌激素上调LRP1 6基因表达的一个增强子元件— 1 2ERE Sp1 ,ERα和Sp1蛋白需要与DNA结合形成复合体 。

LRP16基因启动子活性分析

本研究的目的在于分析LRP16基因不同启动子区域的活性,为深入研究LRP16基因的表达调控机制奠定基础。在NCBI的人类基因组数据库中截取并下载LRP16基因转录起始位点5'侧翼区2.7kb的基因组序列,设计PCR引物,从健康外周血单个核细胞中克隆和亚克隆了LRP16基因的启动子分子,对各个长度相差400bp左右的亚克隆启动子片段调控荧光素酶表达的作用强度进行比较分析。结果获得了与GeneBank序列一致、长度为2.6kb的LRP16基因启动子DNA序列,其主要调控序列在LRP16基因转录起始位点5'侧翼区的-200至-600bp。结论:LRP16基因启动子是一个典型的Ⅱ型启动子,其启动子活性在-200至-600bp区域最强。

LRP16通过调控E-钙黏着蛋白的表达促进MCF-7细胞的侵袭生长

LRP16在原代乳腺癌组织中表达水平与雌激素受体α(ERα)表达状态以及腋窝淋巴结侵袭数目密切相关.为研究LRP16基因对乳腺癌MCF7细胞侵袭生长的影响,并探讨涉及的分子机制,采用基质胶黏附实验与Transwell方法,检测内源性LRP16表达抑制MCF7细胞的体外黏附、侵袭生长与迁移特征.结果表明,抑制LRP16在MCF7细胞中的表达,降低了细胞的体外黏附、侵袭与迁移能力;采用FVB小鼠进行的实验性转移试验结果显示,抑制LRP16显著降低了MCF7细胞的肺转移结节数目;为探索可能的分子机制,采用Western印迹方法,检测了LRP16对乳腺癌转移相关分子MMP2,MMP9,CD44和E钙黏着蛋白表达的影响,结果在LRP16抑制的MCF7细胞中只有E钙黏着蛋白蛋白表达上调.进一步的Northern印迹与免疫组化实验结果表明,抑制LRP16可上调MCF7细胞中E钙黏着蛋白基因的mRNA与蛋白表达水平;共转染与双荧光素酶方法检测LRP16对E钙黏着蛋白基因启动子的表达调控效应,结果显示,LRP16抑制E钙黏着蛋白基因基因5′近端启动子的转录激活,该抑制效应选择性存在于内源性ERα阳性细胞,并且依赖于雌激素的存在;染色质免疫共沉淀方法(ChIP)检测ERα与E钙黏着蛋白基因启动子的相互作用,结果显示,在LRP16基因表达缺陷的MCF7细胞中,ERα抗体沉淀到E钙黏着蛋白基因启动子的DNA序列;上述研究结果表明,抑制LRP16基因表达,削弱了激素依赖型乳腺癌细胞的侵袭生长能力,其分子机制涉及了LRP16通过ERα介导对E钙黏着蛋白基因基因转录激活的调控.

雌激素调控子宫内膜癌Ishikawa细胞中LRP16基因表达及其意义

目的:探讨子宫内膜癌Ishikawa细胞中雌激素(E2)对LRP16基因表达的调控作用,LRP16基因过表达对Ishikawa细胞增殖及侵袭生长能力的影响以及可能的分子机制.方法:采用Northernblot方法检测细胞中LRP16基因mRNA表达水平.双荧光报告系统检测相对荧光素酶活性.胎盘蓝死活细胞计数方法观察LRP16过表达对Ishikawa细胞增殖的影响,Matrigel包被的Transwell方法观察LRP16过表达对Ishikawa细胞侵袭生长能力的影响.Westernblot方法检测Ishikawa细胞中的蛋白水平.结果:雌二醇诱导了Ishikawa细胞中LRP16基因mRNA水平表达上调;而ICI182780则Ishikawa细胞中LRP16mRNA水平下调.增加ERα表达量促使Ishikawa细胞中LRP16基因上调.pGL3S5与ERα共转染Ishikawa细胞的相对荧光素酶活性较单纯pGL3S5转染组细胞升高30倍.我们没有观察到LRP16基因过表达对Ishikawa细胞的促增殖效应.Transwell结果显示LRP16基因过表达Ishikawa细胞的侵袭率较对照组细胞增加了30%.LRP16基因过表达的Ishikawa细胞中E钙粘合素的mRNA以及蛋白水平较对照组细胞下调了3倍,而没有检测到MMP2,MMP9以及CD44蛋白水平的变化.结论:我结果表明E2通过激活ERα上调子宫内膜癌Ishikawa细胞中LRP16基因mRNA水平,LRP16表达水平依赖于雌激素.LRP16基因表达上调没有促进子宫内膜癌细胞的增殖,但可能通过抑制E钙粘合素表达水平增加了细胞的侵袭能力.