The Use of Genetic Approach to the Kinematics of Cutting

This article deals with the use of an interdisciplinary approach to modelling and creation of a complex technical system of different physical nature in relation to the kinematics of cutting and shaping. The professor of the National Technical University of Ukraine, Kuznetcov Iu. N., proposed the approach based on generalization of knowledge, methodological basis of which is the theory of evolution of the systems and methods of genetic analysis and synthesis. For generalization of the knowledge in the fundamental sciences is based on the principles of a limited number of elementary generic structures with the introduction of the gene concept. The modelling and synthesis of kinematic cutting schemes are providing the efficiency and viability of genetic and morphological approach. The material point, which can interact with other ma-terial points in space and time, simulating anthropogenic system of different origin, is introduced as a material object.

Genetic Approaches for Sex Determination of Chinese Female with Male Pseudohermaphroditism

Male pseudohermaphroditism is a rare disorders of sex development(DSD)that is manifested by a female-like appearance or incompletely differentiated external genitalia in an individual with a Y chromosome.In this paper,we report our investigation of the case of a 33-year-old Chinese female who was diagnosed with a malignant mixed germ-cell tumor of the ovary.To confirm the sex of the female,we utilized genetic approaches to detect amelogenin and Y-STR loci.Y chromosome microdeletion was performed to identify existing deletions in the AZF regions and SRY.Chromosome karyotyping and whole-exome sequencing(WES)were then applied to reveal the deletion of sex chromosome segments and pathogenic variations in DNA sequences.Using DNA-STR genotyping,we detected both AMEL-X and AMEL-Y fragments.We also found haplotype Y-STR loci and detected all alleles.Furthermore,no microdeletion was detected in the AZF regions and SRY.The chromosome karyotyping was 46,XY.WES revealed a transversion mutation of 58T→C in the androgen receptor exon 1,which could be the pathogenic variant in this case of abnormal sexual development.Sex determination in forensic DNA typing is based on the amelogenin system.It is important that forensic biologists should master various genetic approaches to overcome the issue of gender ambiguity caused by DSDs.